HUMAN FSH-RECEPTOR BINDING INHIBITORS

人类 FSH 受体结合抑制剂

基本信息

批准号：
2194452
负责人：
DANIEL W LEE
金额：
$ 7.56万
依托单位：
ALBANY MEDICAL COLLEGE
依托单位国家：
美国
项目类别：
财政年份：
1992
资助国家：
美国
起止时间：
1992-03-01 至 1995-02-28
项目状态：
已结题

项目摘要

A. The Candidate Dr. Daniel W. Lee obtained the M.D. degree in 1982 and is Board Certified in Internal Medicine and also in the subspecialty of Endocrinology and Metabolism. He is currently the recipient of a National Research Service Award (NIH) for the period 11/01/88 - 10/31/91. The present proposal is for a Clinical Investigator Award (5 years) to support his further development as an independent clinical investigator. B. The Research The principle goal of this project is to purify and study a previously identified large molecular weight FSH-receptor binding inhibitor (FSH-BI) from human follicular fluid (hFF) which may be involved in the autocrine or paracrine regulation of follicular maturation. The specific goals of the project are to: 1) purify human FSHBI from hFF, 2) biologically characterize hFSH-BI by studying its agonist/antagonist properties as well as its effects on various systems known to respond to FSH, 3) develop an assay for measurement of hFSH-BI in hFF, 4) determine its cell of origin, 5) clone and sequence FSH-BI and 6) study FSH-BI synthesis and expression in follicles of varying maturity. C. The Environment Dr. Leo E. Reichert, Jr., Professor of Biochemistry and Sponsor, has had a long standing interest in gonadotropin hormone and receptor chemistry and mechanism of action and in factors that affect the hormone-receptor interaction (see bibliography). His laboratory is well equipped to support research directed toward the principle goals of the project requiring protein and peptide isolation, characterization of FSH-BI by radioreceptor and radioimmunoassays and by tissue culture. In addition, the facilities of a modern Department of Biochemistry will be available for conduct of the research. The Chairman of the Department, Dr. Kelvin Davies, is a recognized molecular biologist and together with Dr. John Jeffrey (see CV), will provide the expertise needed in related areas of proposed research.

A. 候选人 Daniel W. Lee 博士于 1982 年获得医学博士学位，现任董事会成员获得内科医学和亚专业认证内分泌学和新陈代谢。他目前获得了国家研究服务奖 (NIH) 期间 11/01/88 - 91 年 10 月 31 日。本提案适用于临床研究者奖励（5年）以支持他作为一名独立的临床研究者。 B. 研究该项目的主要目标是纯化和研究先前鉴定的大分子量 FSH 受体结合来自人卵泡液 (hFF) 的抑制剂 (FSH-BI)，可能是参与卵泡的自分泌或旁分泌调节成熟。该项目的具体目标是：1）净化来自 hFF 的人类 FSHBI，2) 通过以下方式对 hFSH-BI 进行生物学表征研究其激动剂/拮抗剂特性及其作用在已知对 FSH 做出反应的各种系统上，3) 开发一种检测方法为了测量 hFF 中的 hFSH-BI，4) 确定其细胞起源，5) 克隆和序列 FSH-BI 和 6) 研究 FSH-BI 不同成熟度的卵泡中的合成和表达。 C. 环境 Leo E. Reichert, Jr. 博士，生物化学教授和发起人，对促性腺激素有着长期的兴趣并且受体化学和作用机制以及影响因素影响激素-受体相互作用（参见参考书目）。他的实验室设备齐全，可以支持针对以下方向的研究需要蛋白质和肽的项目的主要目标通过放射受体分离、表征 FSH-BI 放射免疫测定法和组织培养法。此外，现代化生物化学系的设施将可用于进行研究。主席 Kelvin Davies 博士是一位公认的分子科系主任。生物学家并与约翰·杰弗里博士（见简历）一起，将提供拟议的相关领域所需的专业知识研究。