Disclipine A - Analytical Track 4: Chemistry Capability/Capacity Development

学科 A - 分析轨道 4：化学能力/能力发展

基本信息

批准号：
10160666
负责人：
TIMOTHY JOHN HERRMAN
金额：
$ 13.5万
依托单位：
TEXAS A&M AGRILIFE RESEARCH
依托单位国家：
美国
项目类别：
财政年份：
2020
资助国家：
美国
起止时间：
2020-09-01 至 2025-06-30
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10160666
关键词：
Disclipine Analytical Track Chemistry Capability

项目摘要

Abstract PROJECT FOUR: Capacity Expansion: Texas Whole Genome Sequencing of Isolates from Animal Food Office of the Texas State Chemist (OTSC) has the ability to screen and/or confirm the following organisms: Salmonella spp. in animal food, animal food ingredients, biosolids, human food; Shiga like toxin producing E.coli (STEC) O157:H7 and non-O157 in animal food, animal food ingredients and human food; Listeria monocytogenes in raw milk, animal food and animal food ingredients, human food; SEB in raw milk, Brucella spp. antigen in raw milk, ricin toxin via Tetracore ELISA, Shigella spp. in human food, Bacillus anthracis sterne in human food, Staphylococcal enterotoxins in human food, T-2 toxin and alpha- amanitin by LCMSMS. The microlab received BSL3 training in screening methodologies of Bacillus anthracis and Yersinia pestis at USAMRIID facility, Ft Detrick, MD in 2018 and are moving forward to establish this method (screening and confirmatory) at OTSC facility. The OTSC whole genome sequencing capability development project includes: 1) purchasing MiSeq sequencing platform and necessary instruments, 2) completing and passing competency assessment, 3) expanding the OTSC ISO/IEC 17025.2017 scope to include genome sequencing. As an outcome of the Capacity Expansion: Texas Whole Genome Sequencing of Isolates from Animal Food project, OTSC will begin DNA sequencing using a MiSeq internally within the lab for Listeria and Salmonella DNA, which will strengthen disease surveillance and epidemiology.

抽象的项目四：能力扩展：德克萨斯州分离株的全基因组测序动物食品德克萨斯州化学家办公室 (OTSC) 有能力筛选和/或确认以下内容生物：沙门氏菌属。动物食品、动物食品成分、生物固体、人类食品；动物食品、动物食品中产生志贺样毒素的大肠杆菌 (STEC) O157:H7 和非 O157 成分和人类食品；原料奶、动物食品和动物食品中的单增李斯特菌成分，人类食品；生奶中的 SEB，布鲁氏菌属。原料奶中的抗原，蓖麻毒素通过 Tetracore ELISA，志贺氏菌属。人类食品中的炭疽杆菌，通过 LCMSMS 检测人类食品中的葡萄球菌肠毒素、T-2 毒素和 α-鹅膏蕈碱。这 microlab 接受了炭疽杆菌和耶尔森氏菌筛查方法学的 BSL3 培训 2018 年在位于马里兰州德特里克堡的 USAMRIID 设施中检测到鼠疫菌，并正在努力建立这一点 OTSC 设施的方法（筛选和确认）。 OTSC 全基因组测序能力建设项目包括：1）购买MiSeq测序平台和必要的工具，2) 完成并通过能力评估，3) 扩大 OTSC ISO/IEC 17025.2017 范围包括基因组测序。作为结果能力扩张：德克萨斯州动物食品分离物全基因组测序项目， OTSC 将开始使用实验室内部的 MiSeq 对李斯特菌和沙门氏菌 DNA，这将加强疾病监测和流行病学。