Monitoring the development of resistance to the novel insecticide clothianidin in Anopheles gambiae

监测冈比亚按蚊对新型杀虫剂噻虫胺的抗药性发展

• 批准号: 10155410
• 负责人: Colince Kamdem
• 金额: $ 13.5万
• 依托单位: CENTRE FOR RESEARCH IN INFECTIOUS DISEASES - CRID
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-05-01 至 2025-04-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10155410
• 关键词: Active Sites; Address; Africa; Africa South of the Sahara; African; Agriculture; Anopheles Genus; Anopheles gambiae; Area; Cameroon; Collaborations; Communicable Diseases; Country; Culicidae; Dangerousness; Development; Drug Metabolic Detoxication; Effectiveness; Environmental Risk Factor; Enzymes; Evaluation; Formulation; Fostering; Generations; Genes; Genetic; Genomics; Geography; Goals; Incidence; Insecticide Resistance; Insecticides; Institution; International; Intervention; Knowledge; Lead; Malaria; Measures; Medicine; Metabolic; Monitor; Mutation; National Institute of Allergy and Infectious Disease; Outcome; Plasmodium; Play; Population; Predisposition; Public Health; Research; Research Proposals; Residual state; Resistance; Resistance development; Role; Schools; Speed; Technology Transfer; Testing; Transgenic Organisms; World Health Organization; agricultural activity; agricultural region; burden of illness; combat; experimental study; farmer; fight against; functional genomics; genomic locus; genomic tools; intervention cost; meetings; new technology; novel; overexpression; preservation; prevent; programs; resistance gene; vector; vector control; vector mosquito

Project Summary/Abstract Despite ongoing efforts to diversify malaria control interventions with new technologies such as the use of transgenic mosquitoes to suppress or replace wild vector populations, insecticides remain the cornerstone of strategies aimed at reducing the global burden of this disease. In sub-Saharan Africa in particular, the massive deployment of long-lasting insecticidal nets and indoor residual spraying has contributed to a significant reduction in malaria incidence in just two decades. For this reason, the development of insecticide resistance currently observed among vector populations is regarded as a very serious thread that can quickly undermine recent gains in malaria control. To preserve the effectiveness of insecticides used for malaria vector control, target interventions informed by sufficient knowledge of the susceptibility profile of local populations are more than crucial. Our project aims to study the development of resistance to clothianidin, a new insecticide recently approved for indoor residual spraying. To achieve our goal, we will capitalize on an international collaboration between Liverpool School of Topical Medicine in the UK, Centre for Research in Infectious Diseases in Cameroon as well as notional and international partners directly involved in the fight against malaria in Cameroon. This collaboration will foster capacity building and transfer of technologies in order to promote the use state- of-the-art genomic and functional genomic tools to address the underpinnings of insecticide resistance in malaria vectors in endemic countries. The specific aims of our project are: 1) to monitor the susceptibility of An. gambiae to clothianidin in Cameroon, 2) to evaluate the development and establishment of resistance in clothianidin-selected populations of An. gambiae, and 3) to determine the genetic loci underlying resistance to clothianidin. !

项目概要/摘要 尽管不断努力通过新的措施使疟疾控制干预措施多样化 使用转基因蚊子来抑制或替代蚊子等技术 野生媒介种群、杀虫剂仍然是目标战略的基石 减轻这种疾病的全球负担。特别是在撒哈拉以南非洲地区， 大规模部署长效杀虫网和室内残留物 仅仅两年内，喷洒就显着降低了疟疾发病率 几十年。因此，目前杀虫剂抗性的发展 在向量群体中观察到的现象被认为是一个非常严重的线索，可以 迅速破坏最近在疟疾控制方面取得的成果。为了保持有效性 用于控制疟疾病媒的杀虫剂、目标干预措施 对当地人群的易感性状况有足够的了解 比关键。我们的项目旨在研究耐药性的发展 噻虫胺是一种新的杀虫剂，最近被批准用于室内滞留喷洒。到 为了实现我们的目标，我们将利用之间的国际合作 英国利物浦外用医学院传染病研究中心 喀麦隆以及名义和国际合作伙伴的疾病直接 参与喀麦隆抗击疟疾的斗争。此次合作将促进 能力建设和技术转让，以促进国家使用 最先进的基因组和功能基因组工具来解决 流行国家疟疾病媒对杀虫剂的抗药性。具体目标 我们的项目是：1）监测An的敏感性。冈比亚 至 噻虫胺 喀麦隆，2) 评估当地抵抗力的发展和建立 噻虫胺选择的 An 群体。冈比亚，3) 确定遗传 对噻虫胺具有潜在抗性的位点。 ！