CHRONIC FLUORIDE EFFECTS ON BONE STRENGTH IN RATS

慢性氟化物对大鼠骨强度的影响

• 批准号: 2132522
• 负责人: CHARLES H TURNER
• 金额: $ 9.11万
• 依托单位: INDIANA UNIV-PURDUE UNIV AT INDIANAPOLIS
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-06-01 至 1997-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2132522
• 关键词: X ray crystallography; aging; bone; bone density; bone fracture; densitometry; diabetes mellitus; dietary calcium; elasticity; femur; fluorine; laboratory rat; normal ossification; nutrition related tag; renal failure; scanning electron microscopy; sound; spine; tensile strength

DESCRIPTION: Recent epidemiological evidence suggests higher fracture incidence in communities with fluoridated water. It is conceivable that an increase in fractures may be due to a decrease in bone strength caused by chronic fluoride exposure. Pilot data from rats shows that fluoride reduces bone strength more dramatically in older animals. Thus, bone fluoride accumulation may lead to increased fracture risk in older animals. There is also evidence that fluoride incorporation into the skeleton is altered by dietary changes, especially changes in calcium intake.In this application, the Principal Investigator proposes to measure mechanical and material properties of fluorotic bone from femurs and vertebrae of normal and physiologically challenged rats. Bone specimens will be obtained from Dr. Ann Dunipace of the Oral Health Research Institute at Indiana University, who is Principal Investigator of a grant entitled "Effects of fluoride in medically compromised animals". The Principal Investigator proposes to work in collaboration with Dr. Dunipace and test bone specimens from rats maintained under conditions of aging, diabetes, chronic renal failure and nutritional deficiencies. The hypothesis to be tested is that fluoride accumulation in the bone mineral leads to progressively decreased mechanical properties. It is expected that age and diet will alter the severity of the fluoride effect on bone strength. Bones from diabetic rats or rats with chronic renal failure are expected to accumulate more fluoride in their skeletons,resulting in greater bone strength deficit. It is also hypothesized that fluoride ion distribution in the bone mineral will have a direct effect on the mineralization and elasticity of the bone matrix.

描述：最近的流行病学证据表明更高 氟化水社区的断裂发生率。这是 可以想象，骨折的增加可能是由于减少 在慢性氟化物暴露引起的骨强度中。 试点数据 从大鼠表明氟化物会降低骨强度更多 在年龄较大的动物中急剧。因此，氟化骨的积累可能 导致老年动物的骨折风险增加。 也有 证据表明，氟化物掺入骨架中 饮食变化，尤其是钙摄入量的变化。 应用，主要研究者建议测量 股骨的机械和材料特性 以及正常和生理挑战的大鼠的椎骨。 骨 标本将从口腔健康的Ann Dunipace博士获得 印第安纳大学研究所，主要研究员 一项名为“氟化物在医学上受损中的影响的赠款 动物”。主要研究人员建议在 与Dunipace博士的合作，并测试大鼠的骨头标本 保持在衰老，糖尿病，慢性肾衰竭的条件下保持 和营养不足。 要检验的假设是 氟化物在骨矿物质中的积累导致 机械性能降低。预计年龄和饮食会 改变氟化物对骨强度的严重程度。 骨头 从糖尿病大鼠或患有慢性肾衰竭的大鼠中 在骨骼中积聚更多的氟化物，导致更大的骨头 力量不足。 还假设氟离子 骨矿物质中的分布将具有直接影响 关于骨基质的矿化和弹性。