TOPOGRAPHY OF GLYCOSYLATION IN THE ENDOPLASMIC RETICULUM

内质网糖基化的拓扑结构

• 批准号: 2291423
• 负责人: CARLOS Benjamin HIRSCHBERG
• 金额: $ 2.46万
• 依托单位: UNIV OF MASSACHUSETTS MED SCH WORCESTER
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-04-01 至 1995-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2291423
• 关键词: biological transport; carbohydrate biosynthesis; cytoplasm; dolichol; endoplasmic reticulum; galactose oxidase; glucose; glycosylation; liposomes; membrane activity; membrane lipids; membrane proteins

The purpose of this application for grant support is to allow a collaborative study on the topography of glycosylation reactions in the rough endoplasmic reticulum between Dr. Carlos Hirschberg's laboratory (University of Massachusetts Medical Center, Worcester, MA) and Dr. Armando Parodi's laboratory (Fundacion Compomar, Buenos Aires, Argentina). The long term goals of this proposal are to understand how precursors in protein and lipid glycosylation can be translocated across the membrane of the endoplasmic reticulum, from the cytosolic side to the lumenal side. This translocation is believed to play an important regulatory role in protein and lipid glycosylation. The Specific Aims of this proposal are to determine whether dolichol-P- glucose is synthesized on the cytosolic side of the endoplasmic reticulum membrane and if so, by what mechanism this lipid-saccharide is subsequently translocated across the membrane into the lumen where it serves as a substrate for further glycosylation reactions. These studies will complement ongoing research in Dr. Hirschberg's laboratory dealing with the cell biology, biochemistry & molecular biology of glycosylation reactions, with Dr. Parodi's laboratory on the chemistry and biochemistry of dolichol oligosaccharides.

此拨款支持申请的目的是允许 糖基化反应拓扑的合作研究 Carlos Hirschberg 博士实验室之间的粗面内质网 （马萨诸塞大学医学中心，马萨诸塞州伍斯特）和 Armando 博士 Parodi 的实验室（Fundacion Compomar，布宜诺斯艾利斯，阿根廷）。 这 该提案的长期目标是了解前体如何 蛋白质和脂质糖基化可以跨膜转运 内质网，从胞质侧到腔侧。 这种易位被认为在以下方面发挥着重要的调节作用： 蛋白质和脂质糖基化。 该提案的具体目标是确定 dolichol-P- 葡萄糖在内质网的胞质侧合成 如果是的话，这种脂糖随后通过什么机制 跨膜转移到管腔中，在那里它充当 进一步糖基化反应的底物。 这些研究将 补充 Hirschberg 博士实验室正在进行的研究 细胞生物学、糖基化反应的生物化学和分子生物学， 与 Parodi 博士的多醇化学和生物化学实验室合作 寡糖。