MODEL DIURNAL CIRCADIAN MAMMAL

昼夜节律哺乳动物模型

• 批准号: 3430083
• 负责人: THERESA M LEE
• 金额: $ 7.35万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3430083
• 关键词: Rodentias; alternatives to animals in research; biological models; body physical activity; body temperature; circadian rhythms; cues; model design /development; social behavior; visible light

The primary purpose of the research described is to determine whether the Octodon degus, a South American, diurnal, hystricimorph rodent is a suitable animal model of diurnal circadian rhythms to eventually use in studies of human circadian disorders and research related to the general neurobiological control of circadian rhythms in diurnal mammals. Previous attempts to develop a diurnal animal model have been limited by difficulty obtaining sufficient animals, circadian rhythms were sufficiently noisy so as to exclude studies of the formal properties of the circadian system, behaviors in the laboratory did not agree with those reported in the field, or methodologies were limited for gathering and analyzing data. To date, few researchers have attempted to find a suitable day-active species for extensive description of the formal circadian properties. Development of a diurnal rodent model will allow a more cost-effective means than using human subjects or primates to develop treatments for human disorders such as the problems that result from transmeridian jet travel, shiftwork, illness and hospitalization for traumatic problems. These situations cause deterioration of mental alertness, sleep disruption, ulcers and, in the most severe cases, depression. A good diurnal rodent model will also allow extensive comparative physiological and neurobiological studies. Thus, the main objective of this project is the determination of the formal circadian properties of the degu to determine whether it may prove to be a model diurnal species. The data currently available for diurnal species indicate that circadian function in such species may differ substantially from that of nocturnal species. To determine the formal circadian properties, the following parameters will be examined in adult male and female degus: 1) The stability and precision of entrained circadian activity rhythms. 2) The reentrainment response (time and form) to various size phase-shifts in the light-dark (LD) cycle. 3) Stability of free-running rhythms and their dependence on constant levels of illumination (Aschoff's Rule). 4) Development of phase response curves (PRC's) using 1 hr light pulses for animals free- running in constant darkness (DD). 5) The limits of entrainment (various LD ratios). 6) Relative importance of LD amplitude in entrainment. 7) Comparison of activity and temperature rhythm entrainment and free- running rhythms. 8) Ability of social cues to act as a non-photic entraining agent.

所描述的研究的主要目的是确定是否是否 Octodon degus，南美，昼夜，雌雄同体啮齿动物是一个 合适的昼夜节奏动物模型最终用于 人类昼夜节律的研究与一般有关的研究 昼夜哺乳动物中昼夜节律的神经生物学控制。 以前开发昼夜动物模型的尝试已受到限制 难以获得足够的动物，昼夜节律是 足够嘈杂，以排除对形式特性的研究 昼夜节律，实验室中的行为不同意 在现场报告的人或方法的收集限制 并分析数据。 迄今为止，很少有研究人员试图找到 合适的日间活性物种，用于广泛描述形式 昼夜节律。 昼夜啮齿动物模型的开发将允许更具成本效益 手段比使用人类受试者或灵长类动物开发 人类疾病，例如Transmeridian Jet引起的问题 出行，班次，疾病和住院治疗创伤问题。 这些情况会导致精神警觉性恶化，睡眠 破坏，溃疡，在最严重的情况下是抑郁症。 好 昼夜啮齿动物模型还将允许广泛的比较生理 和神经生物学研究。 因此，该项目的主要目的是确定 DEGU的正式昼夜节目以确定是否可以证明 成为模型的昼夜物种。 当前可用于昼夜的数据 物种表明这种物种中的昼夜节律功能可能有所不同 基本上来自夜间物种。 要确定正式的昼夜节目，以下参数 将在成年男性和女性中检查：1）稳定性和 夹带的昼夜节律节奏的精度。 2）重新入侵 响应（时间和形式）对灯光黑暗中的各种尺寸换档 （LD）周期。 3）自由节奏及其依赖的稳定性 在恒定的照明水平上（阿斯乔夫的规则）。 4）开发 使用1小时的光脉冲为动物自由 - 在恒定的黑暗（DD）中奔跑。 5）夹带的极限（各种 LD比）。 6）LD振幅在夹带中的相对重要性。 7） 活动和温度节奏夹带和自由的比较 运行节奏。 8）社会提示充当非态度的能力 插入代理。