QUANTITATIVE ASSESSMENT OF SUSTAINED ALCOHOL USAGE

持续饮酒的定量评估

• 批准号: 2044631
• 负责人: SAMAR E MAKHLOUF
• 金额: $ 24.3万
• 依托单位: IMMTECH INTERNATIONAL, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2044631
• 关键词: affinity chromatography; alcoholic beverage consumption; alcoholism /alcohol abuse; analytical chemistry; analytical method; blood chemistry; carbohydrate structure; electrofocusing; enzyme linked immunosorbent assay; human tissue; immunological substance; ion exchange chromatography; laboratory mouse; laboratory rabbit; method development; monoclonal antibody; oligosaccharides; protein isoforms; protein purification; protein structure; transferrin

Alcohol usage over time results in a number of metabolic alterations including the production and secretion into the blood of increased quantities of pI 5.7 and 5.9 isoforms of transferrin. These transferrin isoforms (TIs) can be detected by isoelectric focusing and their quantities directly correlate with the amount of alcohol intake by an individual over a time period related to the turnover rate of transferrin in blood; the Tls diminish back to normal levels after alcohol intake is stopped. Thus, quantitation of the Tls is clinically useful for initial estimation of alcohol usage and for monitoring individuals under treatment for alcoholism disease. Present tests for TIs are cumbersome and time-consuming. We have discerned the probable molecular difference between the native transferrin and the TIs, and have prepared reagents that discern the molecular difference of the TIs. The purpose of this proposal is to construct quantitative assays for the Tls in serum specimens, validate the quantities of TIs with our assay system compared to the isoelectric focusing method, and to establish the clinical usefulness of the assay in the testing of individuals suffering from alcoholism as well as possible correlations with other parameters of compromised liver function. The assay will be cost effective and thus should find wider usage in the clinical management of alcoholism.

随着时间的推移，饮酒会导致许多代谢变化 包括增加血液中的产生和分泌 转铁蛋白的 pI 5.7 和 5.9 亚型的数量。 这些转铁蛋白 同种型 (TI) 可以通过等电聚焦来检测，并且它们的 酒精摄入量与酒精摄入量直接相关 个体在一段时间内的转铁蛋白周转率相关 在血液中；酒精摄入量减少后，Tls 会恢复到正常水平 停了下来。 因此，Tls 的定量在临床上对于初始诊断是有用的。 估计酒精使用情况并监测个人 治疗酒精中毒疾病。 目前的 TI 测试很麻烦 并且耗时。 我们已经辨别出可能的分子差异 天然转铁蛋白和TI之间，并已准备好试剂 辨别 TI 的分子差异。 这样做的目的 建议对血清中的 Tls 进行定量分析 样本，与我们的检测系统进行比较，验证 TI 的数量 等电聚焦方法，并建立临床 该测定在测试患有以下疾病的个体中的有用性 酗酒以及与其他参数的可能相关性 肝功能受损。 该测定将具有成本效益，因此 应该在酒精中毒的临床治疗中得到更广泛的应用。