QUANTITATIVE ASSESSMENT OF SUSTAINED ALCOHOL USAGE

持续饮酒的定量评估

• 批准号: 2044631
• 负责人: SAMAR E MAKHLOUF
• 金额: $ 24.3万
• 依托单位: IMMTECH INTERNATIONAL, INC.
• 依托单位国家: 美国
• 财政年份: 1993
• 资助国家: 美国
• 起止时间: 1993-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2044631
• 关键词: affinity chromatography; alcoholic beverage consumption; alcoholism /alcohol abuse; analytical chemistry; analytical method; blood chemistry; carbohydrate structure; electrofocusing; enzyme linked immunosorbent assay; human tissue; immunological substance; ion exchange chromatography; laboratory mouse; laboratory rabbit; method development; monoclonal antibody; oligosaccharides; protein isoforms; protein purification; protein structure; transferrin

Alcohol usage over time results in a number of metabolic alterations including the production and secretion into the blood of increased quantities of pI 5.7 and 5.9 isoforms of transferrin. These transferrin isoforms (TIs) can be detected by isoelectric focusing and their quantities directly correlate with the amount of alcohol intake by an individual over a time period related to the turnover rate of transferrin in blood; the Tls diminish back to normal levels after alcohol intake is stopped. Thus, quantitation of the Tls is clinically useful for initial estimation of alcohol usage and for monitoring individuals under treatment for alcoholism disease. Present tests for TIs are cumbersome and time-consuming. We have discerned the probable molecular difference between the native transferrin and the TIs, and have prepared reagents that discern the molecular difference of the TIs. The purpose of this proposal is to construct quantitative assays for the Tls in serum specimens, validate the quantities of TIs with our assay system compared to the isoelectric focusing method, and to establish the clinical usefulness of the assay in the testing of individuals suffering from alcoholism as well as possible correlations with other parameters of compromised liver function. The assay will be cost effective and thus should find wider usage in the clinical management of alcoholism.

随着时间的推移，饮酒会导致许多代谢改变 包括生产和分泌成增加的血液 pi 5.7和5.9同工型的转铁蛋白的量。 这些转铁蛋白 同工型（TIS）可以通过等电聚焦及其 数量与酒精摄入量直接相关 与转铁蛋白的周转率有关的一个时间段 在血液中；酒精摄入后，TLS降低到正常水平 停了下来。 因此，TLS的定量在临床上对初始有用 估算酒精的使用和监视个人 酗酒疾病的治疗。 目前对TI的测试很麻烦 和耗时。 我们发现了可能的分子差 在天然转铁蛋白和TIS之间，并制备了试剂 辨别tis的分子差。 这个目的 建议是在血清中构建TLS的定量测定 标本，与我们的测定系统验证TI的数量 进行等电聚焦方法，并建立临床 测定在测试患者的个人中的有用性 酒精中毒以及可能与其他参数的相关性 肝功能受损。 该测定将具有成本效益，因此 应该在酒精中毒的临床管理中找到更广泛的用法。