PNEUMOCOCCAL POLYSACCARIDE REFERENCE LABORATORY

肺炎球菌多糖参考实验室

基本信息

批准号：
2297295
负责人：
GERALD SCHIFFMAN
金额：
$ 26.17万
依托单位：
SUNY DOWNSTATE MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-06-28 至 1993-06-27
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2297295
关键词：
PNEUMOCOCCAL POLYSACCARIDE REFERENCE LABORATORY

项目摘要

The overall objective of this proposal is to maintain a Pneumococcal Polysaccharide Serological Reference Laboratory for the purpose of providing a standardized reproducible quantitative system for analyses of antibody responses to pneumococcal polysaccharide vaccines as well as determination of antibody levels during the course of natural disease, surgical intervention (such as splenectomy), drug or radiation therapy, HIV infections and other assaults on the immune system. The Pneumococcal Polysaccharide Reference Laboratory will be capable of performing radioimmunoassay analyses on sera derived from NIAID approved projects in excess of 75,000 assays per year under conditions that the results will be comparable from study to study, from year to year. The Pneumococcal Polysaccharide Reference Laboratory will have available 14C internally labelled biosynthetic pneumococcal capsular polysaccharide of each of the 23 serotypes present in the current pneumococcal vaccine. These reagents will be available to investigators at the request of the project officer. The Pneumococcal Polysaccharide Reference Laboratory will have available purified pneumococcal polysaccharides, unlabelled, available for other investigators wishing to perform ELISA assays and for use in inhibition type measurements. The Pneumococcal Polysaccharide Reference Laboratory will also have available lyophilized preparations of each of the 82 pneumococcal serotypes which can be used to prepare radioactive antigens, unlabeled capsular polysaccharides, vaccines for rabbit immunization to be used for typing or subtyping and for other studies such as opsonization, mouse protection or purification of specific antibody. The Pneumococcal Polysaccharide Reference Laboratory will have available a complete set of 48 typing sera, obtained from Staten Serum Institute, Copenhagen, Denmark, for typing organisms isolated from patients, or to check the authenticity of organisms used in collaborative studies such as opsonization, phagocytosis or mouse protection tests.

该提案的总体目标是维持肺炎球菌多糖血清学参比实验室目的是提供标准化的可重复性用于分析抗体反应的定量系统肺炎球菌多糖疫苗及其测定自然疾病、手术过程中的抗体水平干预（如脾切除术）、药物或放射治疗、HIV 感染和其他对免疫系统的攻击。这肺炎球菌多糖参考实验室将能够对血清进行放射免疫分析来自 NIAID 批准的项目每年超过 75,000 次检测在研究结果具有可比性的条件下学习，年复一年。肺炎球菌多糖参考实验室将有可用的 14C 内部标记生物合成的肺炎球菌荚膜多糖目前的肺炎球菌疫苗有 23 种血清型。这些试剂将根据要求提供给研究人员项目官员。肺炎球菌多糖参考文献实验室将有可用的纯化肺炎球菌多糖，未标记，可供其他研究人员使用希望进行 ELISA 测定并用于抑制型测量。肺炎球菌多糖参考文献实验室还将提供以下冻干制剂 82 种肺炎球菌血清型中的每一种都可用于制备放射性抗原、未标记的荚膜多糖、兔子免疫疫苗用于分型或亚型分析和其他研究，例如调理作用、小鼠特异性抗体的保护或纯化。这肺炎球菌多糖参考实验室将有提供全套 48 种分型血清，从 Staten 获得丹麦哥本哈根血清研究所，用于生物分型从患者身上分离，或检查生物体的真实性用于协作研究，例如调理作用、吞噬作用或鼠标保护测试。