BIOCHEMICAL AND CYTOGENEITC MARKERS IN RETINOBLASTOMA

视网膜母细胞瘤的生化和细胞遗传学标志物

• 批准号: 2096086
• 负责人: WILLIAM Francis BENEDICT
• 金额: $ 19.03万
• 依托单位: BAYLOR COLLEGE OF MEDICINE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1996-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2096086
• 关键词: BIOCHEMICAL; CYTOGENEITC; MARKERS; RETINOBLASTOMA

The long term objective of this proposal is to understand the role of the retinoblastoma (RB) gene, namely loss of RB function, in the development of human tumors as well as its potential to be a prognostic indicator of the clinical outcome in patients with tumors in which the loss of RB function has occurred. Initial studies will concentrate on such tumors as cancers of the lung, breast, bladder, bone, ovary and kidney as well as acute myeloblastic leukemia. Whenever possible normal tissue from the same site will be examined as will metastatic lesions from the same patient when available. Major emphasis will be placed on examining the RB status of human tumors at the single cell level in frozen and paraffin sections as well as direct Western immunoblotting, although many of the tumors will be examined in addition at the RNA and DNA levels. The latter will be done in our attempt to find the structural changes that have occurred within the RB gene to cause the loss of function and to confirm and complement our protein studies. The use of paraffin sections will be done whenever possible since we have recently been successful in obtaining excellent immunohistochemical RB staining of paraffin sections using our polyclonal RB antibody, which, to our knowledge, is not possible with any commercially available RB antibody, whether polyclonal or monoclonal. The fulfillment of the goals of this proposal should provide a much better understanding of the role of the RB gene in some of the most common human malignancies and equally or perhaps even more importantly determine whether monitoring the RB status in patients with specific malignancies will provide significant information related to their clinical outcome or the aggressiveness of their disease.

该提案的长期目标是了解 视网膜母细胞瘤 (RB) 基因，即 RB 功能丧失， 人类肿瘤的发展及其作为预后预测的潜力 肿瘤患者临床结果的指标，其中 RB 功能丧失。 初步研究将集中于 诸如肺癌、乳腺癌、膀胱癌、骨癌、卵巢癌等肿瘤 肾病以及急性粒细胞白血病。 只要有可能就正常 将检查同一部位的组织以及转移性病变 来自同一患者（如有）。 主要重点将放在检查人类肿瘤的 RB 状态 在冷冻和石蜡切片的单细胞水平以及 直接西方免疫印迹，尽管许多肿瘤将被 另外还检查了 RNA 和 DNA 水平。 后者将完成 在我们试图发现内部发生的结构性变化的过程中 RB基因导致功能丧失并确认和补充 我们的蛋白质研究。 石蜡切片的使用将在任何时候进行 这是可能的，因为我们最近成功地获得了优秀的 使用我们的方法对石蜡切片进行免疫组织化学 RB 染色 多克隆 RB 抗体，据我们所知，这是不可能的 任何市售的 RB 抗体，无论是多克隆还是 单克隆。 实现本提案的目标应该提供很多 更好地了解 RB 基因在一些最重要的疾病中的作用 常见的人类恶性肿瘤，同样甚至更重要的是 确定是否监测特定患者的 RB 状态 恶性肿瘤将提供与其相关的重要信息 临床结果或疾病的侵袭性。