GENETIC ANALYSIS OF HEMOPOIETIC HISTOCOMPATIBILITY

造血组织相容性的遗传分析

• 批准号: 2094790
• 负责人: ICHIRO NAKAMURA
• 金额: $ 19.07万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-04-01 至 1995-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2094790
• 关键词: chromosome walking; genetic library; genetic mapping; genetic recombination; genetically modified animals; haploidy; hematopoietic tissue transplantation; histocompatibility; histocompatibility gene; homologous transplantation; immunogenetics; laboratory mouse; major histocompatibility complex; molecular cloning; natural killer cells; northern blottings; nucleic acid probes; restriction fragment length polymorphism; southern blotting; transfection

DESCRIPTION: (Adapted from the applicant's abstract) The long-term objective of this project is to understand the genetic mechanism(s) underlying the natural resistance to hemopoietic allografts. As an initial approach towards the stated objective, three specific aims are proposed: 1) to determine the location of the Hh-1 locus in the H-2-b and H-2-d chromosomes by determining restriction fragment length polymorphism (RFLP) in a panel of H-2S/D recombinant haplotypes, 2) to identify and isolate the Hh-1 genes, using human and murine probes for the H-2S/D interval and H-2-b and H-2-d cosmid libraries, and 3) to initiate the characterization of the genes thus identified. The proposed approach should provide new insight into the genetic mechanism underlying natural resistance and the recognition function of NK cells. In addition, these studies should help identify homologous genes in man, improve the prospect of clinical bone marrow transplantation, and possibly develop NK cell-mediated cancer immunotherapy.

项目成果

A physical map of the mouse H2 Bat5/Db interval.

小鼠 H2 Bat5/Db 间隔的物理图。

• DOI: 10.1007/s003359900343
• 发表时间: 1997
• 期刊: Mammalian genome : official journal of the International Mammalian Genome Society
• 作者: Dagenais,SL;Nakamura,I
• 通讯作者: Nakamura,I

Murine NK cell allospecificity-1 is defined by inhibitory ligands.

鼠 NK 细胞同种异体特异性 1 由抑制性配体定义。

• 发表时间: 1996
• 期刊: Journal of immunology (Baltimore, Md. : 1950)
• 作者: Zheng,WP;Kiura,K;Milisauskas,VK;DeNardin,E;Nakamura,I
• 通讯作者: Nakamura,I

Immunological screening of homologous recombination in genes that encode surface antigens.

编码表面抗原的基因中同源重组的免疫学筛选。

• 发表时间: 1995
• 期刊: BioTechniques
• 影响因子: 2.7
• 作者: Zheng,W;Nakamura,I
• 通讯作者: Nakamura,I

The ability of H-2Dd molecule to affect natural resistance to hemopoietic allografts is an intrinsic property shared by Ddm1 but not Ld.

H-2Dd 分子影响造血同种异体移植天然抗性的能力是 Ddm1 所共有的固有特性，但 Ld 没有。

• DOI: 10.1002/eji.1830240210
• 发表时间: 1994
• 期刊: European journal of immunology
• 影响因子: 5.4
• 作者: Milisauskas,VK;Nakamura,I
• 通讯作者: Nakamura,I

Dd is the only gene that controls NK cell resistance of heterozygous H-2b/d cells.

Dd是唯一控制杂合H-2b/d细胞的NK细胞抗性的基因。

• DOI: 10.1016/0161-5890(95)00095-v
• 发表时间: 1995
• 期刊: Molecular immunology
• 影响因子: 3.6
• 作者: Zheng,WP;Kiura,K;Nakamura,I
• 通讯作者: Nakamura,I