INTERACTIONS BETWEEN INTERMEDIATE FILAMENTS AND NUCLEUS

中间丝和细胞核之间的相互作用

• 批准号: 2088199
• 负责人: ROBERT D GOLDMAN
• 金额: $ 18.88万
• 依托单位: NORTHWESTERN UNIVERSITY AT CHICAGO
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-06-01 至 1998-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2088199
• 关键词: cell cell interaction; cell cycle; cell membrane; cell nucleus; confocal scanning microscopy; electron microscopy; immunofluorescence technique; intermediate filaments; laboratory rabbit; lamins; microinjections; phosphorylation; protein biosynthesis; protein structure function; tissue /cell culture

We propose to continue studying the intermediate filament (IF) based cytoskeletal system in mammalian cells. Emphasis will be placed on the regulation of the organization of IF and their interactions with the nuclear surface. The long term goal of these studies is to determine the possible functions of the IF system and its interaction with the nucleus, now known to contain another IF protein family system known as the nuclear lamins. The experimental plan involves detailed morphological studies on the coordinated activities of the cytoplasmic IF and nuclear surface systems throughout the cell cycle using immunocytochemical techniques, biochemical and physiological analyses, the effects of phosphorylation on the cytoplasmic IF system, structural studies of the nuclear lamin paracrystals, and the use of microinjection of derivatized IF proteins. The IF system is thought to be involved in various physiological activities, including nuclear-cytoplasmic interactions, signal processing between the cell surface and the nucleus, etc. With regard to disease processes, this system is frequently found in an altered state in many diseases including cancer, Alzheimers disease, liver diseases and several other neurological disorders.

我们建议继续研究中间细丝（如果） 基于哺乳动物细胞的细胞骨架系统。 重点将是 放在组织及其组织的监管上 与核表面的相互作用。 长期目标的 这些研究是确定IF的可能功能 系统及其与细胞核的相互作用，现在已知包含 另一个被称为核层蛋白的蛋白质家族系统。 实验计划涉及有关形态学的详细研究 细胞质IF和核的协调活性 整个单元周期的表面系统使用 免疫细胞化学技术，生化和生理 分析，磷酸化对细胞质的影响 系统，核层固定阶层的结构研究以及 使用蛋白质的微分注射的使用。 如果 人们认为系统参与了各种生理 活动，包括核总质相互作用，信号 细胞表面和细胞核之间的处理，等等。 关于疾病过程，该系统经常在 许多疾病的状态改变，包括癌症，阿尔茨海默氏症 疾病，肝病和其他几种神经系统疾病。