CD6--TRANSCRIPTIONAL REGULATION & CELLULAR INTERACTIONS

CD6--转录调控

• 批准号: 2077553
• 负责人: NORA SINGER
• 金额: $ 9.07万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 1995
• 资助国家: 美国
• 起止时间: 1995-04-01 至 2000-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2077553
• 关键词: CD antigens; CD3 molecule; T lymphocyte; antigen presenting cell; autoimmunity; cell adhesion; cell adhesion molecules; cell cell interaction; genetic library; genetic transcription; human tissue; immunopathology; keratinocyte; monoclonal antibody; nucleic acid hybridization; nucleic acid probes; protein structure function; receptor binding; rheumatoid arthritis; southern blotting; thin layer chromatography; transfection

This application proposes funding with the specific intent of developing an independent research program by the principal investigator. The applicant has received clinical training in the fields of pediatrics and internal medicine and is a board certified internist and pediatrician. She is currently completing subspecialty training in the fields of pediatric rheumatology and adult rheumatology and has pursued her interest in basic science research in the areas of T cell immunology and autoimmune disease. This training is in preparation of an academic career as a rheumatologist/immunologist with a special interest in autoimmune diseases of children and young adults, and their immunopathogenesis. This research proposal examines the role of CD6, a glycoprotein expressed on T lymphocytes, in autoreactivity and in cell-cell interactions, including binding of antigen presenting cells in peripheral blood, T cells. Experiments are also proposed to examine the regulation of CD6. This investigator's previous work on CD6, suggests the existence of a CD6 ligand on antigen presenting cells in peripheral blood on synoviocytes and on keratinocytes. This applicant has demonstrated that anti-CD6 monoclonal antibodies inhibit both antigen specific and autoreactive response of human cloned T lymphocytes, as well as binding of CD6+ cells to not-T cells. This is vitro model of autoreactivity appears to have relevance to human autoimmunity. The applicant is now in a position to develop her own independent research program as a junior faculty member of the Department of Internal Medicine, with continuing connections to the Department of Pediatrics. It is expected that the applicant will be promoted to a tenure track junior faculty position in the Departments of Pediatrics and Internal Medicine during the time of the award. The sponsor, who is the head of the Division of Rheumatology, is committed to contributing protected time and resources to the applicant. Additionally the proposed collaborators have demonstrated expertise in the proposed areas of research and in the training of investigators. The applicant's long term goals are to participate in an academic medical community as a rheumatologist with a combined appointment in both a Department of Pediatrics and Internal Medicine. Furthermore, as a basic scientist she hopes to study areas of basic immunology and molecular genetics in order to advance understanding of autoimmune disease. Eventually, she hopes to provide a basic science laboratory environment for pediatric rheumatology trainees in order to contribute to knowledge in areas relevant to pediatric rheumatic diseases.

本申请提出了具有开发特定意图的资金 首席研究员的独立研究计划。这 申请人在儿科领域接受了临床培训 内科医学，是董事会认证的内科医生和儿科医生。 她目前正在完成该领域的专科培训 小儿风湿病学和成人风湿病学并追求她 对T细胞免疫学领域的基础科学研究的兴趣和 自身免疫性疾病。这项培训是为了准备学术生涯 作为对自身免疫感特别感兴趣的风湿病学家/免疫学家 儿童和年轻人的疾病及其免疫发作。 该研究建议研究了CD6的作用，一种表达的糖蛋白 在T淋巴细胞，自动反应性和细胞相互作用中， 包括在外周血中抗原呈现细胞的结合，t 细胞。还提出了实验来检查CD6的调节。 这位研究者先前在CD6上的工作表明CD6的存在 在滑膜细胞上的外周血中的抗原呈现细胞上的配体 以及角质形成细胞。该申请人证明了抗CD6 单克隆抗体抑制抗原特异性和自动反应性 人克隆的T淋巴细胞的反应以及CD6+细胞的结合 非T细胞。这是自动反应性的体外模型 与人类自身免疫有关。 申请人现在可以发展自己的独立 研究计划是内部学会的初级教师 医学，与儿科系保持着持续的联系。 预计申请人将被提升为终身 小学和内部部门的初级教师职位 奖励期间的医学。赞助商，他的负责人 风湿病学的划分，致力于贡献受保护的时间 和资源给申请人。另外提议的合作者 已经在拟议的研究领域和 培训调查人员。 申请人的长期目标是参加学术医学 作为风湿病学家的社区，在两个人中任命合计 儿科和内科系。此外，作为基本 她希望研究基本免疫学和分子的领域 遗传学以提高对自身免疫性疾病的了解。 最终，她希望提供一个基本的科学实验室环境 为小儿风湿学学员而言，为了知识做出贡献 在与小儿风湿病有关的地区。