MYCOPLASMA GENOME ORGANIZATION

支原体基因组组织

• 批准号: 2068152
• 负责人: KENNETH F. BOTT
• 金额: $ 21.29万
• 依托单位: UNIV OF NORTH CAROLINA CHAPEL HILL
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-01 至 1997-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2068152
• 关键词: Mycoplasma; bacterial genetics; biochemical evolution; genetic library; genetic mapping; genome; molecular cloning; nucleic acid hybridization; nucleic acid sequence; pulsed field gel electrophoresis

Genomic analysis using DNA sequence of random clones coupled with hybridization to electrophoretically separated DNA fragments has recently provided an unusual insight into the physiology, metabolism and evolutionary relationships of Mycoplasma genitalium (this laboratory). Of added significance is the fact that this highly fastidious organism possesses the smallest genome size of any free living species. With the knowledge that this methodology provides a valuable aid to assess phylogeny and taxonomy coupled with a powerful probe for analysis of the minimal essential genes required for independent self-replicating existence, we intend to expand this study to include classical M. fermentans (incognitus) plus other mycoplasmas, with special reference to those species having clinical significance to man. This approach will provide important clues to the metabolic pathways these organisms share, the nutrients a parasitic mode provides (missing biosynthetic pathways), and an aid for evaluation of the importance of various conserved genes and pathogenicity determinants. It will also reveal the chromosomal distribution of genes on the respective genomes and provide a new basis from which to assess the relatedness of mycoplasmas to each other and to higher gram positive prokaryotes. All of these studies can be accomplished with a few micrograms of DNA from each species, eliminating the necessity for repeated laboratory cultivation of these highly fastidious pathogens.

使用随机克隆的 DNA 序列进行基因组分析 最近，通过电泳分离 DNA 片段进行杂交 提供了对生理学、新陈代谢和 生殖支原体的进化关系（本实验室）。的 更重要的是，这种高度挑剔的生物体 拥有所有自由生物物种中最小的基因组大小。随着 了解该方法为评估提供了宝贵的帮助 系统发育和分类学以及强大的探针分析 独立自我复制所需的最少必需基因 存在，我们打算扩大这项研究以包括经典 M. 发酵菌（incognitus）和其他支原体，特别是 这些物种对人类具有临床意义。 这种方法将 为这些生物体共有的代谢途径提供重要线索， 寄生模式提供的营养物质（缺少生物合成途径）， 以及帮助评估各种保守基因的重要性 和致病性决定因素。 它还将揭示染色体 基因在各自基因组上的分布并提供新的基础 从中评估支原体之间的相关性并 高等革兰氏阳性原核生物。 所有这些研究都可以 用每个物种的几微克 DNA 来完成， 消除了在实验室重复培养这些物质的必要性 高度挑剔的病原体。