NADPH-BINDING PROTEIN OF THE RESPIRATORY BURST OXIDASE

呼吸爆发氧化酶的 NADPH 结合蛋白

• 批准号: 2065866
• 负责人: Bernard M Babior
• 金额: $ 24.78万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-01-01 至 1995-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2065866
• 关键词: NAD(H) phosphate; SDS polyacrylamide gel electrophoresis; affinity chromatography; affinity labeling; atomic absorption spectrometry; binding proteins; cell free system; cell membrane; chronic granulomatous disease; complementary DNA; electron spin resonance spectroscopy; enzyme induction /repression; enzyme linked immunosorbent assay; enzyme reconstitution; enzyme structure; high performance liquid chromatography; human subject; hybridomas; immunoprecipitation; ion exchange chromatography; laboratory mouse; laboratory rabbit; leukocyte oxidative burst; molecular cloning; molecular genetics; monoclonal antibody; neutrophil; nucleic acid sequence; polymerase chain reaction; protein purification; respiratory enzyme; southern blotting; western blottings

The respiratory burst oxidase is the enzyme responsible for the production of O2- by stimulated phagocytes. A highly complex enzyme composed of many component parts, it catalyzes the oneelectron reduction of oxygen to O 2- at the expense of NADPH. One of its components is an NADPH-binding polypeptide that is found in the cytosol of resting neutrophils but is transferred to the plasma membrane when the cells are activated, thereby assembling the electron transport chain of the enzyme. The goals of the project described in this application are the purification and characterization of this NADPH-binding component and the study of its interaction with other components of the oxidase. Through this work it may ultimately be possible to develop a sufficiently detailed molecular understanding of the operation of the respiratory burst oxidase to allow the development of a new class of anti-inflammatory agents that work by specifically controlling the production of reactive oxidants by phagocytes.

呼吸爆发氧化酶是负责 受刺激的吞噬细胞产生 O2-。 高度复杂的酶 由许多组成部分组成，它催化一个电子还原 以NADPH为代价将氧气转化为O 2- 。 它的组成部分之一是 NADPH 结合多肽存在于静息细胞质中 中性粒细胞，但当细胞处于 被激活，从而组装酶的电子传递链。 本申请中描述的项目的目标是 该 NADPH 结合成分的纯化和表征以及 研究其与氧化酶其他成分的相互作用。 通过 这项工作最终可能会开发出一个足够的 对呼吸系统运作的详细分子理解 爆发氧化酶以允许开发一类新的 抗炎药通过专门控制 吞噬细胞产生活性氧化剂。