M FERMENTANS AND THE PROGRESSION OF HIV INFECTION

M FERMENTANS 和 HIV 感染的进展

• 批准号: 2068194
• 负责人: GAIL H. CASSELL
• 金额: $ 26.38万
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 财政年份: 1992
• 资助国家: 美国
• 起止时间: 1992-08-01 至 1997-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2068194
• 关键词: AIDS; HIV infections; Mycoplasma; T lymphocyte; chronic renal failure; disseminated intravascular coagulation; host organism interaction; human immunodeficiency virus; human tissue; immunosuppression; microorganism interaction; monocyte; opportunistic infections; pathologic process; polymerase chain reaction; virus replication

Within the past 24 months, Mycoplasma fermentans, strain incognitus, has been detected in tissues taken at autopsies from AIDS patients with respiratory, central nervous system (CNS), and renal disease. At the 1991 general meeting of the American Society for Microbiology, increased incidence of M. fermentans was shown in the urine of AIDS patients compared to age and sex-matched non-AIDS patients. In addition, M. fermentans and other mycoplasmas have been reported in the blood of AIDS patients. More recently mycoplasmas, especially M. fermentans strain incognitus, have even been proposed to be cofactors for HIV infection. In fact regulatory mechanisms of replication of human immunodeficiency virus (HIV) in vitro do suggest molecular mechanisms by which mycoplasmas could increase virus production in infected individuals. The mitogenic properties of mycoplasmas could increase HIV production in infected lymphocytes. Indeed other mitogens that act on T cells are known to enhance HIV production in vitro. Increased virus production is known to be associated with clinical progression in HIV-infected individuals. However, the incidence, natural history, pathogenic potential, and clinical significance of M.fermentans in AIDS patients is unknown. In addition, previous epidemiologic investigations have not yet demonstrated any clear association between progression of ADDS and any putative cofactor. Our long term goals are to determine if M. fermentans is either a cofactor in HIV infection or an important opportunistic pathogen. If M.fermentans is either, it then becomes important to determine the natural history of the infection. The specific aims of the present application are to: (i) identify sites of M.fermentans localization in autopsy tissues of AIDS patients and other patients with immunosuppression or other chronic debilitating diseases; (ii) identify HIV infected individuals who are co-infected with M. fermentans and identify matched controls who are HIV positive, M. fermentans negative; and (iii) determine if the rate of change in various parameters, including increase in HIV replication, decline in CD4+ lymphocytes or alterations in other lymphocyte subpopulations, and loss of immune responsiveness is greater in HIV-infected individuals with M. fermentans infection as compared to HIV-infected non-M.fermentans infected matched controls.

在过去的24个月中 在艾滋病患者的尸检中检测到 呼吸道，中枢神经系统（CNS）和肾脏疾病。 在 1991年美国微生物学会股东大会增加 在艾滋病患者的尿液中显示了发酵杆菌的发病率 与年龄和性别匹配的非AIDS患者相比。 此外，M。 艾滋病的血液中已经报道了发酵罐和其他支原体 患者。 最近，分枝杆菌，尤其是Fermentans菌株 隐身，甚至被认为是艾滋病毒感染的辅助因子。 实际上，人类免疫缺陷复制的调节机制 体外病毒（HIV）确实提出了分子机制 可能会增加感染个体的病毒产生。有丝分裂 支原体的特性可能会增加感染的HIV产生 淋巴细胞。 实际上，已知其他作用于T细胞的有丝分裂剂 在体外增强HIV产生。 已知病毒产生的增加是 与感染HIV的个体的临床进展有关。 但是，发生率，自然病史，致病潜力和 艾滋病患者的临床意义尚不清楚。 在 此外，以前的流行病学研究尚未证明 添加的进展与任何推定的任何明确关联 辅因子。 我们的长期目标是确定Fermentans M.是否是 艾滋病毒感染中的辅助因子或重要的机会主义 病原。 如果M. fearmentans是 确定感染的自然病史。 特定目标 目前的申请是：（i）识别M.Furementans的地点 在艾滋病患者和其他患者的尸检组织中定位 免疫抑制或其他慢性衰弱疾病； （ii）确定 艾滋病毒感染的人与发酵芽孢杆菌共同感染 识别艾滋病毒阳性的匹配对照，发酵犬M. Farmentans阴性； （iii）确定各种参数的变化率是否 包括增加HIV复制，CD4+淋巴细胞的下降或 其他淋巴细胞亚群的改变和免疫丧失 在患有发酵杆菌的艾滋病毒感染者中，反应性更大 与感染HIV的非M.Etremmentans感染的感染相比 控件。