MORPHOGENESIS OF THE MOSQUITO PATHOGEN LAGENIDIUM

蚊子病原体 Lagenidium 的形态发生

• 批准号: 2062047
• 负责人: JAMES L KERWIN
• 金额: $ 17.99万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1995-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2062047
• 关键词: Culicidae; arthropod nonpollutant control; biotechnology; eicosanoids; enzyme mechanism; fatty acid metabolism; fermentation; field study; growth media; histogenesis; life cycle; lipoxygenase; oxygenases; phospholipids; plant growth /development; plant physiology; protein structure function; sterols

Resistance developed by mosquitoes to available chemical insecticides and restrictions on their use favor the development of host-specific microbial agents for operational mosquito control. The proposed research continues investigation of fermentation production, storage, activation and application of the sexual stage (oospores) of Lagenidium giganteum. This is the only eukaryotic biological control agent of medically important arthropods with immediate potential for use in operational control programs. Conventional approaches to yield optimization using stirred tank fermentation will focus on alteration will focus on alteration of culture parameters at specific developmental stages of the fungus. Response surface analysis will be used to accelerate the development of optimum oospore yields. Modification of fermentation conditions to optimize synthesis of specific compounds associated with oosporogenesis will be attempted. Storage and activation protocols will be modified to limit premature abortion of converted oospores. Multi- hectare field tests will continue, including in areas in which mosquito- borne disease is a severe health problem. This sterol auxotrophic fungus requires an exogenous source of sterols to enter its reproductive cycle. Precise structural requirements for bulk and regulatory roles of sterols will be determined. Phospholipids, including sphingolipids which regulate enzymic activity, are involved in the control of L. giganteum growth and morphogenesis. The effect of sterols on phospholipid uptake, synthesis and metabolism will be examined. Sterol regulation of eicosanoid and related oxygenated fatty acid metabolism will be investigated and correlated with specific developmental stages of the fungus. Eicosanoids are mediators of the inflammatory response in mammals. Use of this fungus as a model system will provide the background for increasing oospore yields for commercial fermentation production of L. giganteum, and for future investigations of lipid-mediated enzymic activity.

蚊子对可用的化学杀虫剂和 使用他们使用的限制有利于特定于主机的发展 用于操作蚊子控制的微生物剂。 拟议的研究 继续研究发酵生产，存储，激活 和lagenidium giganteum的性阶段（卵子）的应用。 这是医学上唯一的真核生物控制药物 重要的节肢动物具有直接使用的操作潜力 控制程序。 使用的常规方法使用 搅拌坦克发酵将集中于更改 在特定发展阶段的培养参数改变 真菌。 响应表面分析将用于加速 开发最佳的卵子产量。 发酵的修饰 优化与之相关的特定化合物合成的条件 将尝试卵子发生。 存储和激活协议将 修改以限制转化后的卵子的过早流产。 多- 公顷现场测试将继续，包括在蚊子的地区 传播疾病是一个严重的健康问题。 这种固醇可营养的真菌需要外源性的固醇来源 输入其生殖周期。 精确的结构要求 将确定固醇的体积和调节作用。 磷脂， 包括调节酶活性的鞘脂参与 控制巨球生长和形态发生的控制。 效果 固醇磷脂摄取，合成和代谢将是 检查。 花生醇的二十烷和相关氧化脂肪调节 酸代谢将被研究并与特定 真菌的发展阶段。 eicosanoids是 哺乳动物的炎症反应。 将这种真菌用作模型系统 将为商业商业的增加的O纵收益率提供背景 L. giganteum的发酵生产以及未来的调查 脂质介导的酶活性。

项目成果

Identification of molecular species of glycerophospholipids and sphingomyelin using electrospray mass spectrometry.

• 发表时间: 1994-06
• 期刊: Journal of lipid research
• 影响因子: 6.5
• 作者: James L. Kerwin;A. R. Tuininga;Lowell H. Ericssont

Effects of exogenous phospholipids on lipid composition and sporulation by three strains of Lagenidium giganteum.

外源磷脂对三株巨型 Lagenidium giganteum 脂质组成和孢子形成的影响。

• DOI: 10.1016/0022-2011(91)90187-u
• 发表时间: 1991
• 期刊: Journal of invertebrate pathology
• 影响因子: 3.4
• 作者: Kerwin,JL;Duddles,ND;Washino,RK
• 通讯作者: Washino,RK

Nonmammalian safety tests for Lagenidium giganteum (Oömycetes: Lagenidiales).

Lagenidium giganteum（真菌纲：Lagenidiales）的非哺乳动物安全性测试。

• DOI: 10.1093/jee/81.1.158
• 发表时间: 1988
• 期刊: Journal of economic entomology
• 影响因子: 2.2
• 作者: Kerwin,JL;Dritz,DA;Washino,RK
• 通讯作者: Washino,RK

Pilot scale production and application in wildlife ponds of Lagenidium giganteum (Oomycetes: Lagenidiales).

Lagenidium giganteum（卵菌纲：Lagenidiales）在野生动物池塘中的中试规模生产和应用。

• 发表时间: 1994
• 期刊: Journal of the American Mosquito Control Association
• 影响因子: 1
• 作者: Kerwin,JL;Dritz,DA;Washino,RK
• 通讯作者: Washino,RK

Isoprenoid-mediated changes in the glycerophospholipid molecular species of the sterol auxotrophic fungus Lagenidium giganteum.

• DOI: 10.1099/13500872-141-2-399
• 发表时间: 1995-02
• 期刊: Microbiology
• 影响因子: 1.5
• 作者: James L. Kerwin;A. R. Tuininga;Alison M. Wiens;Jennifer C. Wang;Jessica J. Torvik;Misty L. Conrath;Joanna K. MacKichan