Functional studies of presynaptic proteins in neurotransmitter release

突触前蛋白在神经递质释放中的功能研究

• 批准号: 09480225
• 负责人: MOCHIDA Sumiko
• 金额: $ 6.72万
• 依托单位: Tokyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09480225/
• 关键词: synaptic vesicles; neurotransmitter; exocytosis; prosynaptic proteins; CaィイD12+ィエD1-binding proteins; CaィイD12+ィエD1 channels; Ca^<2+>チャネル

To examine roles for presynaptic protein-protein interactions in neurotransmitter release, synapses formed between rat superior cervical ganglion neurons in culture were employed. The synaptic protein interaction site of several presynaptic proteins were synthesized and introduced into the presynaptic neurons through a glass patch pipette, while measuring synaptic transmission evoked by presynaptic action potentials. The change in postsynaptic responses, we speculated the function of the proteins as follows.1)CaィイD12+ィエD1-binding protein(A)synaptotagmin : trigger of synaptic vesicle fusion, promotion of endocytosis.(B)Doc2α-Munc 13 interacion : synaptic vesicle translocation to the release site.2)SNARE-associating proteins(A)N-type CaィイD12+ィエD1 channels : involved in synchronous transmitter release, voltage-dependent enhancement of transmitter release.(B)P/Q-type CaィイD12+ィエD1 channels : involved in neurotransmitter release.cSnapin : modulation of synaptotagmin-SNARE complex interaction.(D)Syntaphilin : control of SNARE complex assembly by syntaxin-1 clamping.

为了检查突触前蛋白质-蛋白质相互作用在神经递质释放中的作用，利用培养物中的大鼠颈上神经节神经元之间形成的突触，合成了几种突触前蛋白质的突触蛋白质相互作用位点，并通过玻璃贴片吸管引入突触前神经元。通过测量突触前动作电位引起的突触传递，我们推测蛋白质的功能为： 1)CaiiD12+yeD1结合蛋白(A)突触结合蛋白：触发突触小泡融合，促进内吞作用。(B)Doc2α-Munc 13相互作用：突触小泡易位至释放位点。2)SNARE相关蛋白(A) )N型CaD12+D1通道：参与同步发射机释放，电压相关增强递质释放。(B)P/Q 型 CaiD12+D1 通道：参与神经递质释放。cSnapin：调节突触结合蛋白-SNARE 复合体相互作用。(D)Syntaphilin：通过 Syntaxin-1 钳位控制 SNARE 复合体组装。

项目成果

Shunji Kozaki: "Ganglioside GTlb as complementary receptor for clostridial botulinum neurotoxins"Microb. Pathog. 25・2. 91-99 (1998)

Shunji Kozaki：“神经节苷脂 GTlb 作为肉毒杆菌神经毒素的互补受体”，Pathog 25·2。

Sumiko Mochida: "Role of Doc2α-Munc13-1 interaction in the neurotransmitter release process"Proc. Natl. Acad. Sci. USA. 95・19. 11418-11422 (1998)

Sumiko Mochida：“Doc2α-Munc13-1 相互作用在神经递质释放过程中的作用”，美国科学院院刊，11418-11422。

Kozaki,S., Kamata,Y., Watari,S., Nishiki,T., Mochida,S.: "Ganglioside GT1b as complementary receptor for clostridial botulinum neurotoxins."Microb. Pathog.. 25(2). 91-99 (1998)

Kozaki,S.、Kamata,Y.、Watari,S.、Nishiki,T.、Mochida,S.：“神经节苷脂 GT1b 作为肉毒杆菌神经毒素的互补受体。”微生物。

Sumiko Mochida: "Slow Synsptic Responses and Modulation"K.Kuba,H.Higashida,D.A.Brown,T.Yoshioka. 455(23) (1999)

Sumiko Mochida：“缓慢的突触反应和调制”K.Kuba，H.Higashida，D.A.Brown，T.Yoshioka。

Mochida,S.: "Protein-protein interactions in neurotransmitter release."Neurosci. Res.. 36(3). 175-182 (2000)

Mochida，S.：“神经递质释放中的蛋白质-蛋白质相互作用。”神经科学。