HYPOTHALAMO-HYPOPHYSIAL SYSTEM AND CONTROL OF GONADOTROPIN SECRETION IN THE ELASMOBRANCHS

下丘脑-垂体系统和软骨素促性腺激素分泌的控制

基本信息

批准号：
09640789
负责人：
NOZAKI Masumi
金额：
$ 2.05万
依托单位：
NIIGATA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1999
项目状态：
已结题

项目摘要

Hypothalamo-hypophysial system was studied in some species of elasmobranchs, such as dogfish Scyliorhinus torazame, and common skate Raja porosa, in relation to the control of gonadotropin secretion. The project was focused on 1) distribution of adenohypophysial hormones in the pituitary, 2) distribution of gonadotropin-releasing hormones (GnRHs) in the brain, 3) isolation of gonadotropin from the ventral lobe, and 4) establishment of radioimmuno-assay of dogfish gonadotropin. During the term of this project the research subject was only completed on the distribution of adenohypophysial hormones. The distribution of GnRH-neuronal system was extensively studied using antibodies to dogfish GnRH and other GnRHs. Immunoreactive GnRH-fibers were abundantly observed in the forebrain, but they were few in number in the hypothalamus. Although it is expected that GnRH reaches the ventral lobe via general circulation, it is still unclear from where GnRH is released to the blood circulation. An a … More ttempt to isolate gonadotropin from the ventral lobe was unsuccessful, probably due to high contamination. The following is the abstract of the distribution of adenohypophysial hormones in the dogfish pituitary. ACTH cells and MSH cells stained with anti-porcine ACTH and anti-α-MSH were found in the rostral pars distalis and pars intermedia, respectively. Growth hormone (GH) cells stained with anti-shark GH and anti-sturgeon GH were found in the proximal pars distalis. Somatolactin cells stained with anti-salmon somatolactin were found in the pars intermedia. However, prolactin cells, if present, were not detected by anti-sturgeon prolactin or anti-salmon prolactin. In the ventral lobe, only one type of cells was stained positively by antibodies to glycoprotein hormones or their subunits, with stronger immuno-reaction by anti-sturgeon GTH IIβ, anti-salmon GTH, anti-hCG, and anti-human TSHβ. Since only one type of glycoprotein hormone cell was detected, it was difficult to know whether both GTH and TSH cells are present there. Less

研究了某些软骨鱼物种（例如角鲨和鳐鱼）的下丘脑-垂体系统与促性腺激素分泌控制的关系。该项目的重点是 1) 腺垂体激素在垂体中的分布，2)。促性腺激素释放激素（GnRH）在大脑中的分布，3）分离4）角鲨促性腺激素放射免疫测定法的建立在本项目期间，研究课题仅完成了腺垂体激素的分布。使用抗体对 GnRH 神经系统的分布进行了广泛的研究。角鲨 GnRH 和其他 GnRH 纤维在前脑中大量观察到，但在脑中数量很少。尽管预期 GnRH 通过全身循环到达腹叶，但仍不清楚 GnRH 从何处释放到血液循环中，从腹叶中分离促性腺激素的尝试未成功，可能是由于高污染。以下是用抗猪ACTH和染色的角鲨垂体ACTH细胞和MSH细胞中腺垂体激素的分布摘要。分别在用抗鲨鱼 GH 染色的头端部和中间部中发现了抗 α-MSH，在用抗鲑鱼染色的近端生长激素细胞中发现了抗鲟鱼 GH。在中间部发现了生长泌乳素，但是，抗鲟鱼催乳素或抗鲑鱼没有检测到催乳素细胞（如果存在）。在催乳素腹叶中，只有一种类型的细胞被糖蛋白激素或其亚基的抗体染色呈阳性，抗鲟鱼GTH IIβ、抗鲑鱼GTH、抗hCG和抗人TSHβ的免疫反应较强。由于只检测到一种类型的糖蛋白激素细胞，因此很难知道那里是否同时存在 GTH 和 TSH 细胞。