Studies on diversity of sheep scrapie agents
羊痒病药剂多样性研究
基本信息
- 批准号:09660312
- 负责人:
- 金额:$ 2.62万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:1997
- 资助国家:日本
- 起止时间:1997 至 1999
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
The agent (prion) of transmissible spongiform encephalopathies (prion diseases) including scrape in sheep, bovine spongiform encephalopathy, and Creutzfeldt-Jakob disease (CJD) in human does not possess the agent-specific nucleic acid as a genome. Therefore genome typing and/or antigenic typing are not applicable for distinction of prion strains. However, prion strains can be distinguishable to some extent by biological and biochemical properties. In this study, we attempted to elucidate whether diversity of sheep prion strains exist in Japan. In addition, we analyzed genetic factor(s) other than PrP which will influence biological properties of prions.Eleven sheep scrapie occurred in Japan were examined in the transmissibility to mice and the relative protease-resistance of sheep PrPィイD2scィエD2, accumulatedin brains. The results indicated that eleven sheep scrapie used here can be divided into three groups on the basis of biological and biochemical properties. This suggests that variou … More s sheep prions exist in the field of Japan. Although characterization of the biological properties of sheep prions in the field has been reported and revealed diversity of sheep prions in UK, this is the first report which elucidated the diversity of sheep prions in Japan. The results obtained here will be useful to assess the possible risk for the spread of sheep prion in Japan to other species.A particular genotype of apolipoprotein E (ApoE) is known as a risk factor for Alzheimer's disease. It is also suggested that a particular genotype of ApoE might influence the disease type of CJD. Thus we examined whether there exists any relation between sheep ApoE and prion diversity or disease type. First we cloned genomic DNA and cDNA of sheep ApoE gene and determined the nucleotide sequence of them, and we identified three sheep ApoE genotypes. However, there was no significant relationship between the polymorphism of sheep ApoE gene and biological properties of sheep prion or disease types. Less
传染性海绵状脑病(朊病毒病)的病原体(朊病毒),包括绵羊擦伤病、牛海绵状脑病和人类克雅氏病(CJD),不具有作为基因组的病原体特异性核酸。或抗原分型不适用于朊病毒株的区分,但是,在本研究中,我们可以通过生物学和生化特性在一定程度上区分朊病毒株。试图阐明日本是否存在绵羊朊病毒株的多样性。此外,我们分析了除PrP之外的影响朊病毒生物学特性的遗传因素。对日本发生的11只绵羊瘙痒病的小鼠传染性和相关性进行了检查。绵羊 PrP2scieD2 的蛋白酶抗性,在大脑中积累。结果表明,这里使用的 11 只绵羊瘙痒症可根据生物学和生化特性分为三组。日本领域存在多种羊朊病毒,尽管英国已报道了羊朊病毒的现场生物学特性并揭示了羊朊病毒的多样性,但这是首次阐明羊朊病毒多样性的报告。日本。这里获得的结果将有助于评估日本羊朊病毒向其他物种传播的可能风险。载脂蛋白 E (ApoE) 的特定基因型被认为是阿尔茨海默病的危险因素。因此我们首先克隆了绵羊ApoE基因的基因组DNA和cDNA,并测定了它们的核苷酸序列。 ,我们鉴定了三种绵羊ApoE基因型,但绵羊ApoE基因多态性与绵羊朊病毒的生物学特性或疾病类型之间没有显着关系。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Komatsu Y.,et al.: "Characterization of the sheep apolipoprotein E (ApoE) gene and allelic variations of the ApoE gene in scrapie Suffolk sheep"Gene. 208. 131-138 (1998)
Komatsu Y.,et al.:“绵羊载脂蛋白 E (ApoE) 基因的特征和萨福克羊痒病中 ApoE 基因的等位基因变异”基因。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Komatsu, Y., Horiguchi, M., Ishiguro, N., Matsui, T., and Shinagawa, M.: "Characterization of the sheep apolipoprotein E(ApoE) gene and allelic variations of the ApoE gene in scrapie Suffolk sheep."Gene. 208. 131-138 (1998)
Komatsu, Y.、Horiguchi, M.、Ishiguro, N.、Matsui, T. 和 Shinakawa, M.:“绵羊载脂蛋白 E (ApoE) 基因的特征以及瘙痒病萨福克羊中 ApoE 基因的等位基因变异。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Komatsua,et al.: "Characterization of the sheep apolipoprotein E(ApoE)gene and allelic varia tions of the ApoE gene in scrapie Suffolk sheep." Gene. 208. 131-138 (1998)
Komatsua 等人:“萨福克羊痒病中绵羊载脂蛋白 E (ApoE) 基因的特征和 ApoE 基因的等位基因变异。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Komatsu Y.et al.: "Characterization of the sheep apolipoprotein E (ApoE) gene and allelic variations of the ApoE gene in scrapie Suffolk sheep"Gene. 208. 131-138 (1998)
Komatsu Y.等人:“萨福克羊痒病中绵羊载脂蛋白 E (ApoE) 基因的特征和 ApoE 基因的等位基因变异”基因。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Komatsu, et al.,: "Characterization of the sheep apolipoprotein E (ApoE) gene and alleic variations of the ApoE gene in scrapie Suffolk sheep." Gene,. (in press).
Komatsu 等人:“萨福克羊痒病中绵羊载脂蛋白 E (ApoE) 基因的特征和 ApoE 基因的等位基因变异。”
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
数据更新时间:{{ journalArticles.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ monograph.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ sciAawards.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ conferencePapers.updateTime }}
{{ item.title }}
- 作者:
{{ item.author }}
数据更新时间:{{ patent.updateTime }}
MATSUI Takane其他文献
MATSUI Takane的其他文献
{{
item.title }}
{{ item.translation_title }}
- DOI:
{{ item.doi }} - 发表时间:
{{ item.publish_year }} - 期刊:
- 影响因子:{{ item.factor }}
- 作者:
{{ item.authors }} - 通讯作者:
{{ item.author }}
相似国自然基金
NSP2朊病毒样特性在PRRSV致肺损伤中作用机制研究
- 批准号:32373007
- 批准年份:2023
- 资助金额:50 万元
- 项目类别:面上项目
Hsp70调控朊病毒蛋白相分离、聚集和毒性的分子机制研究
- 批准号:
- 批准年份:2022
- 资助金额:54 万元
- 项目类别:面上项目
Prion疾病中PINK1-Parkin介导神经元线粒体自噬受损及PINK1失稳机制的研究
- 批准号:
- 批准年份:2022
- 资助金额:54 万元
- 项目类别:面上项目
免疫调节因子Progranulin在Prion致神经退变中的作用和分子机制
- 批准号:
- 批准年份:2022
- 资助金额:52 万元
- 项目类别:面上项目
戊型肝炎病毒ORF3蛋白的朊病毒样特性在病毒致病中的作用
- 批准号:32170165
- 批准年份:2021
- 资助金额:58 万元
- 项目类别:面上项目
相似海外基金
Transmissible Spongiform Encephalopathy (Prion) Disease of Deer and Elk
鹿和麋鹿的传染性海绵状脑病(朊病毒)病
- 批准号:
7251637 - 财政年份:2007
- 资助金额:
$ 2.62万 - 项目类别:
Study on Pathogenesis of prion disease (A role of cytokines in spongiform formation in affected brains)
朊病毒病发病机制研究(细胞因子在受影响大脑海绵状形成中的作用)
- 批准号:
09671002 - 财政年份:1997
- 资助金额:
$ 2.62万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Molecular pathogenesis of transmissible spongiform encephalopathy
传染性海绵状脑病的分子发病机制
- 批准号:
08300011 - 财政年份:1997
- 资助金额:
$ 2.62万 - 项目类别:
Grant-in-Aid for Scientific Research (A)
Transgenic models of transmissible spongiform encephalopathy
传染性海绵状脑病的转基因模型
- 批准号:
7569316 - 财政年份:
- 资助金额:
$ 2.62万 - 项目类别:
Transgenic models of transmissible spongiform encephalopathy
传染性海绵状脑病的转基因模型
- 批准号:
7365119 - 财政年份:
- 资助金额:
$ 2.62万 - 项目类别: