IDENTIFICATION OF NUCLEAR PROTEIN EXPRESSED IN TRANSACTIVATED ITO CELLS

反式激活 ITO 细胞中表达的核蛋白的鉴定

• 批准号: 09670554
• 负责人: SAIBARA Toshiji
• 金额: $ 1.92万
• 依托单位: KOCHI MEDICAL SCHOOL
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670554/
• 关键词: ITO CELLS; LIVER; RAT; STELLATE CELLS; MYOFIBROBLAST; NITRIC OXIDE; CATIONIC AMINO ACID TRANSPORTER

Development of liver fibrosis results in hepatic failure, hepatocellular carcinoma, esophageal varices, and spontaneous peritonitis, which are life-threatening complications in liver cirrhosis. Induction of apoptosis in transformed Ito cells maybe a promising way of treatment of chronic liver disease. A new insight into the changes of nuclear protein expressed in the process of transformation may provide an important information for the treatment of liver fibrosis.Progress in purification of Ito cells enabled us to obtain highly-purified cells and we investigate the process of transformation precisely. In this study, we developed monoclonal antibody for purifying nuclear protein transiently expressed in transformed Ito cells during the process of transformation and the analysis of amino acid sequence revealed it as emelin commonly expressed in myocytes. We need further investigation to recognize the role of transient expression of emelin in Ito cells during transformation.

肝纤维化的发展导致肝衰竭、肝细胞癌、食管静脉曲张和自发性腹膜炎，这些是肝硬化的危及生命的并发症。诱导转化的 Ito 细胞凋亡可能是治疗慢性肝病的一种有前途的方法。对转化过程中核蛋白表达变化的新认识可能为肝纤维化的治疗提供重要信息。Ito细胞纯化的进展使我们能够获得高纯度的细胞，并精确研究转化过程。在本研究中，我们开发了单克隆抗体，用于纯化转化过程中瞬时表达的伊藤细胞核蛋白，氨基酸序列分析表明其为肌细胞中普遍表达的emelin。我们需要进一步研究以认识转化过程中 Ito 细胞中 emelin 瞬时表达的作用。

项目成果

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