Study of the effects of alpha2 adrenoceptor on the development of epileptogenesis in immature rats

α2肾上腺素受体对幼鼠癫痫发生发展影响的研究

• 批准号: 09670990
• 负责人: YOSHIOKA Shin-ichi
• 金额: $ 2.11万
• 依托单位: TOTTORI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670990/
• 关键词: Epileptogenesis; Alpha 2 adrenoceptor; Amygdaloid kindling; Immature period; l-Histidine; Droxydopa; Rat; 幼若; 発達; α2アドレナリン受容体

The imbalance in inhibitory and excitatory neurotransmitters plays an important role in the development of epileptic seizures in human and animal models. Central noradrenergic systems are reported to suppress the development of kindling. However, noradrenergic systems are immature in postnatal period compared with in adult period. Therefore, to elucidate the effect of alpha-2 adrenoceptor on the development of epileptogenesis in immature period, we investigated the effects on the development of amygdaloid kindling in various aged rats (postnatal 14 days, 21 days, 28 days, and 70 days).1. Alpha-2 agonist, clonidine, suppressed the development of kindling in 28-day-old young rats as well as in adult rats. However, the development of kindling was facilitated by clonidine in 14-day-old pup rats.2. Facilitative effect of development of kindling by clonidine in 14-day-old pup rats was blocked with combined administration of clonidine and alpha-2 antagonist such as yohimbine, idazoxan or rauwolscine.3. Noradrenaline precursor droxydopa did not affect the development of kindling in 14-or 21-day-old rats.4. Histamne precursor 1-histidine caused inhibitory effects on the development of kindling in both adult and 14-, 21-day-old pup rats.The present pharmacological studies indicate that the effects of alpha-2 adrenoceptors on the development of amygdaloid kindling are age specific.

抑制性和兴奋性神经递质的不平衡在人类和动物模型中癫痫发作的发展中起着重要作用。据报道，中枢去甲肾上腺素能系统可以抑制火种的发展。然而，与成年期相比，出生后的去甲肾上腺素能系统还不成熟。因此，为了阐明α-2肾上腺素受体对未成熟期癫痫发生发展的影响，我们研究了其对不同年龄大鼠（出生后14天、21天、28天和70天）杏仁核点燃发育的影响。 1. Alpha-2 激动剂可乐定可抑制 28 日龄幼鼠和成年大鼠的点火发育。然而，可乐定对14日龄幼鼠的点火有促进作用。2．可乐定与α-2拮抗剂育亨宾、达唑生、萝芙素联合给药可阻断可乐定对14日龄幼鼠火种发育的促进作用。 3．去甲肾上腺素前体屈羟多巴不影响14日龄或21日龄大鼠的点火体发育。4．组胺前体1-组氨酸对成年和14、21日龄幼鼠的火种发育有抑制作用。目前的药理学研究表明，α-2肾上腺素受体对杏仁火种发育的影响具有年龄特异性。 。

项目成果

Shin-ichi Yoshioka: "Effects of histamine precursor l-histidine on the development of amygdaloid kindling in developing rats" J.Jpn.Epil.Soc. 17. 66 (1999)

Shin-ichi Yoshioka：“组胺前体 L-组氨酸对发育中大鼠杏仁核点火的影响”J.Jpn.Epil.Soc。

吉岡 伸一: "成長段階のラット扁桃核キンドリング形成過程に及ぼすクロニジンの影響" てんかん研究. 16・1. 55-56 (1998)

吉冈真一：“可乐定对生长阶段大鼠杏仁核点燃过程的影响”癫痫研究16・1（1998）。

Seiji Katayama: "The effects of noradrenergic alpha-2 agonist clonidine on the development of amygdaloid kindling in immature rats" J.Yonago Med.Ass.18. 103-115 (1997)

Seiji Katayama：“去甲肾上腺素能 α-2 激动剂可乐定对未成熟大鼠杏仁核点燃发育的影响”J.Yonago Med.Ass.18。

Katayama, S.: "The α2-agonisit clonidine facilitates the development of amygdaloid kindling in infant rats" Electroencephalography and clinical Neurophysiology. 98・4. 52 (1996)

Katayama，S.：“α2-激动剂可乐定促进幼鼠杏仁核的发育”，脑电图和临床神经生理学 98・4（1996）。

S.Katayama: "The alpha2-agonist clonidine facilitates the development of amygdaloid kindling in infant rats." Electroencephalography and clinical Neurophysiolgy. 98. 52 (1996)

S.Katayama：“α2 激动剂可乐定促进幼鼠杏仁核的发育。”