Effects of lidocaine on reperfusion injury of microcirculation -a study using the retinal microvessels-

利多卡因对微循环再灌注损伤的影响-利用视网膜微血管的研究-

基本信息

批准号：
09671592
负责人：
FUJITA Yoshihisa
金额：
$ 1.98万
依托单位：
KAWASAKI MEDICAL SCHOOL
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1997
资助国家：
日本
起止时间：
1997 至 1998
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671592/
关键词：
microcirculation lidocaine leukocytes endothelium adhesion molecules microvessels vital microscopy fluorescence

项目摘要

The purpose of the research project was to examine the effects of lidocaine on leukocyte-endothelium interaction in retinal microcirculation. Because visualization of the retinal microvessels and blood cells flowing in these microvessels was not possible by our system consisting of a fluorescence vital microscope and an image analyzer (Argus 20, Hamamatsu Photonics), we performed the studies in hamsters where the microscopic observation chamber was implanted on the dorsal skin fold and in Sprague-Dawley rats using the scrotal striated muscle. Animals were divided into the lidocaine group (n=7) receiving lidocaine infusion 0.3mg/kg/hr and the control group (n=7) receiving the ame volume of saline. Leukocyte adhesion to the post-venular endothelium was attenuated in the animals of the lidocaine group (plasma lidocaine concentration : 6.1 *3.3 mu g/mL) as compared with those of the control group. The number of rolling leukocytes on the post-capillary venules did not differ among the contr … More ol and lidocaine groups. We concluded that attenuation of the leukocyte adhesion to the post-capillary venule may be responsible for the anti-inflammatory property of the lidocaine.In another study, we tried to visualize the retinal microcirculation in rabbits by using Scanning Laser Ophthalmoscopy (SLO, Rodenstock, Germany). Rabbits with ligation of bilateral carotid artery were anesthetized either with propofol or halothane. The effects of induced hypotension (mean arterial pressure=ca. 50 mmHg) by these anesthetics on retinal microcirculation were compared. Capillary leakage or blockage was observed in neither group, suggesting the safety of propofol anesthesia in ophthalmologic surgery.Although the system permits visualization and quantitative analysis of retinal inicrovessels and their plasma flow by using fluorescein sodium, it could not visualize leukocytes in the retinal microcirculation with rhodamine 6G, a fluorescent dye, which is used for staining leukocytes in microcirculation research. Advances in Laser technology or staining techniques are necessary for investigation of leukocyte hemodynamics in the retinal microcirculation. Less

该研究项目的目的是检查利多卡因对视网膜微循环中白细胞-内皮相互作用的影响，因为我们的由荧光活体显微镜和图像组成的系统无法实现视网膜微血管和这些微血管中流动的血细胞的可视化。分析仪（Argus 20，Hamamatsu Photonics），我们在仓鼠身上进行了研究，其中显微观察室被植入到背部皮肤褶皱和使用阴囊横纹肌的Sprague-Dawley大鼠被分为接受利多卡因输注0.3mg/kg/hr的利多卡因组(n=7)和接受相同体积的白细胞粘附的盐水组。利多卡因组的动物中小静脉后内皮细胞减弱（血浆利多卡因浓度：6.1 *3.3 mu g/mL），如下所示与对照组相比，对照组和利多卡因组中毛细血管后微静脉上滚动的白细胞数量没有差异，我们得出结论，白细胞对毛细血管后微静脉的粘附减弱可能是造成这种情况的原因。利多卡因的抗炎特性。在另一项研究中，我们尝试使用扫描激光检眼镜（SLO，用异丙酚或氟烷麻醉双侧颈动脉结扎的兔子，比较了这些麻醉剂引起的低血压（平均动脉压=约50mmHg）对视网膜微循环的影响，这表明在两组中均未观察到毛细血管渗漏或阻塞。异丙酚麻醉在眼科手术中的安全性。尽管该系统允许视网膜的可视化和定量分析使用荧光素钠观察微血管及其血浆流，无法使用罗丹明 6G（一种荧光染料，用于微循环研究中对白细胞进行染色）来可视化视网膜微循环中的白细胞。激光技术或染色技术的进步对于白细胞的研究是必要的。视网膜微循环血流动力学较差。