Core C: IgSeq and NGS Core

核心 C：IgSeq 和 NGS 核心

基本信息

批准号：
10549607
负责人：
Jiwon Lee
金额：
$ 36.36万
依托单位：
BOSTON CHILDREN'S HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
2011
资助国家：
美国
起止时间：
2011-08-01 至 2028-03-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10549607
关键词：
Antibodies Antibody Repertoire Antibody Response Antigens Attention B cell repertoire B-Lymphocytes B-cell receptor repertoire sequencing DNA Sequencing Facility Data Data Analyses Development Elderly Engineering Exposure to Funding Goals Hemagglutinin Human Humoral Immunities Immune Individual Infant Infection Influenza Influenza Hemagglutinin Influenza vaccination Infrastructure Investigation Length Liquid Chromatography Mediating Memory B-Lymphocyte Molecular Monitor Monoclonal Antibodies Neuraminidase Online Systems Plasmablast Play Population Proteomics Recombinants Research Personnel Research Support Resolution Resources Role Sampling Seasons Sequence Analysis Series Serum Services Shapes Structure Technology Time Transcript Vaccination Vaccines Viral Virus Diseases Work age group analytical tool college data repository database query design imprint influenza virus vaccine next generation sequencing professor programs response seasonal influenza success tandem mass spectrometry tool transcriptomics vaccination strategy young adult

项目摘要

ABSTRACT A principal goal of the Program Project is to chart the changes in influenza antibody repertoire following vaccination or infection and to build on those results to direct immunogen design efforts. Core C, the Ig-Seq and NGS Core, will serve the overall goals of the Program Project by providing high-throughput antibody repertoire profiling services. Specifically, it will provide in-depth NextGen Sequencing (NGS) of antibody transcripts from B cells (BCR-Seq) and quantitative high-resolution proteomic analysis of serum antibody molecules (Ig-Seq). From these sequence analyses, the Core will generate full-length antibody sequences for recombinant expression and characterizations as monoclonal antibodies.The Core will also serve all Projects and Cores of the Program Project by providing a centralized data repository, management of all antibody sequence data, robust and consistent data analysis. The specific aims of Core C are: (1) provide the NGS service to define the B cell repertoire; (2) provide the proteomic-based antibody analysis service to define serum antibody repertoire; (3) provide a centralized data repository and analysis for antibody sequences generated by all the Projects and Cores; and (4) expand and optimize the provided services. The Ig-Seq and NGS Core is critical for the success of the Program Project, offering high-throughput tools for deep and comprehensive monitoring of changes in three distinct components of antibody repertoires: serum antibody molecules, memory B cells, and plasmablasts.

抽象的该计划项目的主要目标是绘制流感抗体库的变化。疫苗接种或感染，并以这些结果为基础，以指导免疫原设计工作。核心C，IG-Seq NGS Core将通过提供高通量抗体来实现该计划项目的整体目标曲目分析服务。具体而言，它将提供深入的抗体的内部测序（NGS）来自B细胞（BCR-SEQ）的转录本和血清抗体的定量高分辨率蛋白质组学分析分子（Ig-Seq）。从这些序列分析中，核心将生成全长抗体序列重组表达和作为单克隆抗体的特征。核心还将服务于所有项目和计划项目的核心，通过提供集中式数据存储库，所有抗体的管理序列数据，鲁棒和一致的数据分析。核心C的具体目的是：（1）提供NGS 定义B细胞曲目的服务；（2）提供基于蛋白质组学的抗体分析服务来定义血清抗体库；（3）为抗体序列提供集中式数据存储库和分析由所有项目和核心产生；（4）扩展和优化提供的服务。 Ig-Seq和 NGS核心对于该计划项目的成功至关重要，为深度和深处提供了高通量工具全面监测抗体库的三个不同组成部分的变化：血清抗体分子，记忆B细胞和浆膜。