Viral Testing Core

病毒检测核心

• 批准号: 10548595
• 负责人: Smriti Mehra
• 金额: $ 3.08万
• 依托单位: TEXAS BIOMEDICAL RESEARCH INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2000
• 资助国家: 美国
• 起止时间: 2000-09-30 至 2027-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10548595
• 关键词: 2019-nCoV; AIDS vaccine development; Acquired Immunodeficiency Syndrome; Affect; Algorithms; Animal Model; Animals; Antibodies; Antibody titer measurement; Antiviral Therapy; Biological Assay; Breeding; COVID-19 pandemic; COVID-19 testing; California; Caribbean region; Chagas Disease; Consensus; Data; Decision Making; Development; Ensure; Equipment; Event; Exposure to; Goals; Grant; HIV; Health; Herpesvirus 1; Human; Human Resources; Immune response; Immunization; Immunization Programs; Immunoassay; Immunology; Infection; Investigation; Laboratories; Macaca; Macaca mulatta; Maintenance; Measles; Measles virus; Methods; Monitor; Monkeys; Mucous Membrane; Oregon; Parasites; Pathogen detection; Patients; Pest Control; Prevalence; Primates; Principal Investigator; Procedures; Reagent; Recombinants; Recommendation; Reliability of Results; Research; Research Personnel; Research Support; Reverse Transcriptase Polymerase Chain Reaction; Rhesus; Risk; SARS-CoV-2 antibody; SIV; Sampling; Serology; Serology test; Serum; Simian Retroviruses; System; Techniques; Testing; Time; Trypanosoma cruzi; U-Series Cooperative Agreements; United States National Institutes of Health; Viral; Viral Pathogenesis; Virus; Washington; animal care; antibody detection; effectiveness evaluation; efficacy evaluation; germ free condition; improved; instrument; interest; member; pathogen; programs; screening; simian human immunodeficiency virus; viral RNA; working group

Abstract – Core B: Viral Testing Core The Viral Testing Core is a critical component for the maintenance of a Specific Pathogen Free (SPF) Indian rhesus macaque (Ind RM) (Macaca mulatta) breeding colony. Rhesus macaques infected with the Simian Immunodeficiency Virus (SIV) are the preferred animal model for AIDS studies and are used to examine many aspects of viral pathogenesis, AIDS vaccine development, and exploratory new systemic and mucosal antiviral therapies, including early events not readily studied in human patients. The presence of certain viruses can confound the results of AIDS-related investigations. SPF colonies have been developed using state-of-the art assays to eliminate animals with simian immunodeficiency virus (SIV), Type D simian retrovirus (SRV) simian T- lymphotropic virus (STLV-1); and herpes B virus (Macacine herpesvirus-1), which is a potential health risk for personnel handling the animals. The goal of the Viral Testing Core is to provide techniques and expertise to assure the continuing SPF status of the Ind RM breeding colony at the Southwest National Primate Research Center (SNPRC). In order to fulfill this goal, the following Specific Aims will be completed: Specific Aim 1: to provide a reliable and sensitive in-house viral screening method. This screening method is intended to produce reliable results in a fast turnaround time. We will continue to use the Charles River Laboratories (CRL) Multiplex Fluorescent ImmunoAssay (FMIA) for serological screening of all animals, which represents one of the current state-of-the-art surveillance techniques. Specific Aim 2: to increase the research value of the SNPRC Ind RM breeding colony by expanding serology testing. The core is ever evolving in the use of state-of-the-art techniques and expanding to detect other pathogens of interest. In recent years, we extended our serology testing to include detection of antibodies against measles virus and Trypanosoma cruzi. Presence of antibodies to measles is used to confirm immune responses to the colony active measles immunization effort. Serological screening for antibodies to the causative agent of Chagas Disease, along with PCR confirmatory testing, is used to 1) assist animal selection; 2) to determine the prevalence of this infection in the NHP colonies; and 3) to improve and evaluate the effectiveness of our pest control system. Finally, SNPRC has recently screened the SPF colony for SARS-CoV-2 by both serology and PCR. Following recommendations developed by the National Primate Research Center Pathogen Detection Working Group, the Viral Testing Core has developed an algorithm that incorporates results from the serological viral screening with both serological and PCR confirmatory assays. Additionally, the Core will continue to participate in activities proposed by the national Breeding Colony Management Consortium (BCMC), which frequently distributes serum panels for proficiency testing among its laboratory members with the intent to monitor and ensure acceptable quality testing algorithms, reagents, methods, equipment, and personnel.

摘要 - 核心B：病毒测试核心 病毒测试核心是维持特定病原体（SPF）印度的关键组成部分 恒河猕猴（Ind RM）（Macaca Mulatta）繁殖菌落。感染了猿猴的恒河猕猴 免疫缺陷病毒（SIV）是艾滋病研究的首选动物模型，用于检查许多 病毒发病机理的各个方面，艾滋病疫苗的发育以及探索性的新系统性和粘膜抗病毒 疗法，包括不容易在人类患者中研究的早期事件。某些病毒的存在可以 混淆了与艾滋病有关的研究结果。 SPF殖民地是使用最先进的 消除具有猿猴免疫缺陷病毒（SIV）的动物的测定 淋巴病毒（STLV-1）；和疱疹B病毒（大麦疱疹病毒1），这是潜在的健康风险 人员处理动物。病毒测试核心的目标是提供技术和专业知识 确保西南国家灵长类动物研究的IND RM繁殖殖民地的持续SPF状态 中心（SNPRC）。为了实现这一目标，将完成以下特定目标：特定目的1： 提供可靠且敏感的内部病毒筛查方法。这种筛选方法旨在产生 可靠的结果很快。我们将继续使用查尔斯河实验室（CRL）多路复用 用于所有动物血清学筛查的荧光免疫测定（FMIA），代表当前之一 最先进的监视技术。特定目的2：增加SNPRC IND RM的研究值 通过扩大血清学测试来繁殖菌落。核心在使用最先进的技术方面正在发展 并扩展以检测其他感兴趣的病原体。近年来，我们将血清学测试扩展到包括 检测针对麻疹病毒和克鲁氏锥虫的抗体。使用麻疹抗体的存在 确认对菌落活性麻疹免疫努力的免疫反应。血清学筛查 使用PCR确认测试的偶然抗体抗体用于1）协助 动物选择； 2）确定NHP菌落中这种感染的患病率； 3）改进和 评估我们的害虫控制系统的有效性。最后，SNPRC最近放映了SPF殖民地 SARS-COV-2通过血清学和PCR。遵循国家灵长类动物提出的建议 研究中心病原体检测工作组，病毒测试核心开发了一种算法， 将血清学病毒筛查的结果与血清学和PCR确认测定法相结合。 此外，核心将继续参加国家育种殖民地提出的活动 管理财团（BCMC），它经常在其之间分发血清面板以进行熟练度测试 意图监视和确保可接受的质量测试算法，试剂，试剂的实验室成员 方法，设备和人员。