Non-Coding RNA Analytical Core

非编码 RNA 分析核心

基本信息

批准号：
10544057
负责人：
Kasey C Vickers
金额：
$ 20.76万
依托单位：
VANDERBILT UNIVERSITY MEDICAL CENTER
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-06-01 至 2025-12-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10544057
关键词：
Academic Medical Centers Area Atherosclerosis Award Bioinformatics Biometry Biotechnology ChIP-seq Chromium Chronic Kidney Failure Collaborations Communication Complex Data Analyses Data Set Dedications Equipment Familial Hypercholesterolemia Functional disorder Funding Genes Genetic Transcription Genomics Goals Grant High Density Lipoproteins High-Throughput Nucleotide Sequencing High-Throughput RNA Sequencing Human Resources Individual Informatics Joints Lead Leadership Libraries Lipoproteins Messenger RNA Methods Modification Multiomic Data Preparation Proteins Protocols documentation Publications Quality Control RNA RNA analysis RNA immunoprecipitation sequencing Recommendation Recording of previous events Research Research Design Research Personnel Resources Ribosomal RNA Science Services Small RNA System Technology Testing Training Transfer RNA Universities Untranslated RNA Work analysis pipeline data integration data management design experience experimental study extracellular human disease laboratory experience member multiple omics next generation programs service programs single-cell RNA sequencing skills success tool transcriptome sequencing transcriptomics

项目摘要

Summary The purpose of this program is to significantly advance our understanding of mechanisms of HDL Dysfunction in Projects (1) familial hypercholesterolemia (2) chronic kidney disease, (3) small RNAs, and (4) reactive dicarbonyl modification to HDL. The proposed research strategies are highly cross-disciplinary and require extensive next-generation RNA sequencing, data analysis, and data integration. An effective and dedicated non- coding RNA and bioinformatics core is essential for the success of the proposed studies in each project and the overall program. Specifically, the Non-Coding RNA and Bioinformatics Core is designed to handle the large volume of sequencing needs and data analyses for all 4 projects. We are highly trained, skilled, and experienced in generating sRNA-seq libraries, bulk total RNA-seq libraries, and 10X Chromium single cell RNA sequencing (scRNA-seq) preparations for high-throughput sequencing. We are proficient in performing quality control steps and completing the sequencing tasks. Furthermore, we have developed in-house sequencing analysis pipelines that will be implemented by computational biologists and biostatisticians under the guidance of an existing informatics team that has worked closely over the past 6 years. The purpose of the Non-Coding RNA and Bioinformatics Core is to use high-throughput RNA sequencing and informatics to analyze small and long RNA expression changes associated with HDL dysfunction and atherosclerosis treatments in a timely and efficiently manner with high-quality data management and integration into complex bioinformatics systems.

概括该计划的目的是显着提高我们对HDL功能障碍机制的理解在项目（1）家族性高胆固醇血症（2）慢性肾脏病，（3）小RNA和（4）反应性 Dicarbonyl修改为HDL。拟议的研究策略是高度跨学科的，需要广泛的下一代RNA测序，数据分析和数据集成。有效而专用的非 - 编码RNA和生物信息学核心对于每个项目中提出的研究的成功至关重要总体计划。具体而言，非编码RNA和生物信息学核心旨在处理大型所有4个项目的测序需求和数据分析的数量。我们是训练有素，熟练和经验丰富的在生成SRNA-seq库，批量总RNA-seq库和10倍铬单细胞RNA测序中（SCRNA-SEQ）用于高通量测序的准备。我们精通执行质量控制步骤并完成测序任务。此外，我们已经开发了内部测序分析管道这将由计算生物学家和生物统计学家在现有的指导下实施在过去的6年中一直紧密工作的信息团队。非编码RNA的目的生物信息学核心是使用高通量RNA测序和信息学来分析小和长RNA 与HDL功能障碍和动脉粥样硬化治疗相关的表达变化及时有效具有高质量的数据管理和集成到复杂的生物信息学系统中的方式。