Establishment of a new extraction method for phytochemicals using the wet stone-mill and non-destructive evaluation method for extract quality based on fluorescence excitation emission matrix

湿式石磨植物化学物质提取新方法的建立及基于荧光激发发射矩阵的提取物质量无损评价方法

• 批准号: 22KJ0403
• 负责人: BUI Thi Bao Chau
• 金额: $ 1.09万
• 依托单位: University of Tsukuba
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for JSPS Fellows
• 财政年份: 2023
• 资助国家: 日本
• 起止时间: 2023-03-08 至 2024-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22KJ0403/
• 关键词: extraction method; wet milling; phytochemicals

The new extraction method named the Simultaneous Milling and Extraction Process (SMEP) has been successfully established using the original wet stone-mill. SMEP was successfully optimized with ethanol 70% as the most suitable extraction solvent and 1 loop milling as the most efficient setting. SMEP at its optimal condition demonstrated significantly higher extraction efficiency in terms of total polyphenols, total flavonoids and antioxidant abilities compared to all comparative extraction methods, even the ultrasonic-based methods with greatly simplified protocol and in shorter time. In addition, the practical usage of SMEP-produced extracts was confirmed with the biochemical assay involving the cancer-related human retinaldehyde dehydrogenase type 3 (RALDH3) enzyme inhibition. SMEP-produced extracts were significantly more active than extracts prepared by other methods. Therefore, the practical usability of SMEP in producing spice extracts of high quality and of SMEP-produced extracts has been confirmed. These results of the first and third objectives in the current project led to publication of an original paper (Bui et al., 2022), an Excellent Poster Award in the 69th JSFST Conference and two invited lectures.

利用原有的湿式石磨成功建立了新的提取方法，即同步研磨和提取工艺（SMEP）。 SMEP 已成功优化，使用 70% 乙醇作为最合适的提取溶剂，1 环研磨作为最有效的设置。与所有比较提取方法相比，在最佳条件下，SMEP 在总多酚、总黄酮和抗氧化能力方面表现出显着更高的提取效率，即使是基于超声波的方法，其方案大大简化，时间更短。此外，通过涉及癌症相关的人视黄醛脱氢酶 3 型 (RALDH3) 酶抑制的生化测定，证实了 SMEP 生产的提取物的实际用途。 SMEP 生产的提取物比其他方法制备的提取物活性明显更高。因此，SMEP在生产高质量香料提取物和SMEP生产的提取物方面的实际可用性已得到证实。当前项目第一和第三个目标的这些成果导致发表了一篇原创论文（Bui et al., 2022）、第 69 届 JSFST 会议优秀墙报奖和两场特邀报告。

项目成果

Simultaneous stone-milling and extraction enables efficient one-step extraction of hard plant materials

同步石磨和提取可实现硬质植物材料的高效一步提取

• DOI: 10.1016/j.ifset.2022.103096
• 发表时间: 2022
• 影响因子: 6.6
• 作者: Bui Thi Bao Chau;Kokawa Mito;Tran Thu Tra;Nosaki Shohei;Miura Kenji;Kitamura Yutaka
• 通讯作者: Kitamura Yutaka

A novel method to extract abundant and trace phytochemicals from spices

从香料中提取丰富微量植物化学物质的新方法

• 发表时间: 2022
• 作者: Bui Thi Bao Chau

Simultaneous Milling and Extraction (SME) a new simple method for extraction of hard plant materials.

同步研磨和提取 (SME) 是一种提取硬质植物材料的新的简单方法。

• 发表时间: 2022
• 作者: Bui Thi Bao Chau

Non-destructive estimation of antioxidant capacity of spice extracts with fluorescence fingerprint

荧光指纹法无损评价香料提取物的抗氧化能力

• 发表时间: 2023
• 作者: Bui Thi Bao Chau; Kitamura Yutaka; Kokawa Mito
• 通讯作者: Kokawa Mito

A novel method to extract hard plant materials using the wet stone-mill.

一种利用湿石磨提取坚硬植物材料的新方法。

• 发表时间: 2022
• 作者: Bui Thi Bao Chau; Kokawa Mito; Tran Thu Tra; Nosaki Shohei; Miura Kenji; Kitamura Yutaka
• 通讯作者: Kitamura Yutaka