Molecular and cellular biological analysis for the effect of glucocorticoids and their recetors on the function and tissue construction of neuron.

糖皮质激素及其受体对神经元功能和组织结构影响的分子和细胞生物学分析。

基本信息

批准号：
08680867
负责人：
OZAWA Hitoshi
金额：
$ 1.28万
依托单位：
Kyoto Prefectural University of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1996
资助国家：
日本
起止时间：
1996 至 1997
项目状态：
已结题

项目摘要

The cellular localization of GRmRNA and GR immunoreactivity in the adult rat brain was studied by in situ hybridization and immunocytochemistry. The expression of GR mRNA was detected in the cytoplasm of neurons and observed in the various regions of the rat brain, especially in the hippocampus, the thalamus, hypothalamus, the cerebral cortex. The distributional pattern of GR-immunoreactive cells was well-correlated with that of GR mRNA-containing cells, but in the hippocampus (CA3/CA4) discrepancy of the distribution of mRNAs and immunoreactivities.Subcellular localization of glucocorticoid receptor (GR) in rat brain was immunocytochemically observed under different conditions of circulating corticosteroids level in conjunction with the specific antibody to GR.In the case of hippocampal pyramidal and granular cells, the GR-immunoreactivity was mainly distributed in the nucleus associated with chromatin in the control (normal) condition. However, the immunoreactivity almost vanished in … More all area of the cells 2 days after adrenalectomy (ADX). After 10 or 15 min of intraperitoneal (i.p.) injection of dexamethasone (DEX ; 200mg/kg) to these ADX rats, the GR-immunoreactivity reappeared in the cytoplasm associated with cell organelles. After 30 min of DEX i.p.injection, the same GR-immunoreactivity was distributed in nucleus as showing the control condition.Moreover, the GRmRNA was abundantly expressed in the cytoplasm of anterior pituitary cells, and of scatteringly posterior pituitary being equivalent to pituicytes. The immunoreactivity for GR was detected in the nuclei of cells in anterior and posterior pituitary. Double immunohistochemistry revealed that co-localization of GR and anterior pituitary hormones were observed in the majority of growth hormone (GH) and adreno-corticotropic hormone (ACTH) producing cells, and in about 60 % of thyroid stimulating hormone (TSH) producing cells. Folliculo-stellate cells, marginal layr cells in the anterior pituitary, and pituicytes in the posterior pituitary, immunostained for S100-protein, were also immunostained with GR.These results suggest that glucocorticoids may directly influence certain pituitary cells and regulate the cell function including the synthesis and/or secretion of hormones. Less

采用原位杂交和免疫细胞化学方法研究了成年大鼠脑内GR mRNA的细胞定位和GR免疫反应性。丘脑、下丘脑、大脑皮层 GR 免疫反应细胞的分布模式与含有 GR mRNA 的细胞的分布模式密切相关，但在海马中。 (CA3/CA4) mRNA 分布和免疫反应性的差异。在不同循环皮质类固醇水平条件下结合 GR 特异性抗体，通过免疫细胞化学观察大鼠脑中糖皮质激素受体 (GR) 的亚细胞定位。以海马锥体为例颗粒细胞的GR免疫反应性主要分布在与染色质相关的细胞核中，而在对照（正常）条件下，免疫反应性几乎不存在。肾上腺切除术 (ADX) 2 天后，所有细胞区域的 GR 免疫反应性在腹膜内 (i.p.) 注射地塞米松 (DEX；200mg/kg) 10 或 15 分钟后，在 ADX 大鼠中重新出现。 DEX 腹腔注射 30 分钟后，细胞核中分布着与对照相同的 GR 免疫反应性。此外，GRmRNA在垂体前叶细胞的细胞质中大量表达，并且在垂体后叶细胞中与垂体细胞相当。双重免疫组织化学在垂体前叶和后叶细胞的细胞核中检测到GR的免疫反应性。在大多数生长激素（GH）和促肾上腺皮质激素（ACTH）产生细胞中观察到 GR 和垂体前叶激素的定位，并且在大约60% 的促甲状腺激素 (TSH) 产生细胞、垂体前叶边缘层细胞和垂体后叶垂体细胞也用 GR 进行免疫染色，进行 S100 蛋白免疫染色。这些结果表明糖皮质激素可能直接影响某些垂体细胞并调节细胞功能，包括激素的合成和/或分泌。