Center for Structural Biology of HIV RNA

HIV RNA结构生物学中心

• 批准号: 10505792
• 负责人: ALICE TELESNITSKY
• 金额: $ 36.09万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-06-09 至 2027-03-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10505792
• 关键词: Area; Biological; Biological Assay; Biological Process; Biology; Biophysics; CRISPR screen; Cells; Cellular biology; Chemicals; Chemistry; Data; HIV; HIV-1; In Vitro; Lead; Machine Learning; Molecular Biology; Proteins; RNA; Research Personnel; Screening procedure; Small Interfering RNA; Structure; Techniques; Validation; base; biophysical analysis; chemical synthesis; cheminformatics; in silico; inhibitor; next generation sequencing; novel; protein complex; screening; small molecule; small molecule inhibitor; structural biology; virology

Core 4: Biology and Chemistry Summary CRNA investigators in this Core component have a wide range of expertise in virology, cell and molecular biology, RNA biology, and chemistry. Each of the biologically focused projects requires the application of techniques and expertise in these areas. To facilitate the identification of new targets for structural and biophysical analysis, the validation and biological interpretation of structural and biophysical data, and the identification of lead small molecule chemical probes, the Biology and Chemistry Core will undertake a number of activities including: (1) the discovery of new features of HIV-1 RNAs and HIV-1 RNA-protein complexes for structure function studies using next-generation sequencing-based approaches, (2) HyPR-MS, siRNA and sCAS9/SAM (CRISPR) screening to identify and validate new protein targets for structure-function studies, (3) the provision of virology and general molecular biology support to biologically and structurally focused projects and aims, and (4) in silico, in vitro and in cell RNA-targeted screening and chemical synthesis along with cheminformatics / machine learning techniques to enable the discovery of RNA-targeted inhibitors of the biological processes studied in the CRNA.

核心 4：生物学和化学 概括 该核心部分的 CRNA 研究人员在病毒学、细胞和分子方面拥有广泛的专业知识 生物学、RNA 生物学和化学。每个以生物学为重点的项目都需要应用 这些领域的技术和专业知识。促进确定结构和目标的新目标 生物物理分析、结构和生物物理数据的验证和生物学解释，以及 先导小分子化学探针的鉴定，生物和化学核心将承担多项 活动包括：(1) 发现 HIV-1 RNA 和 HIV-1 RNA-蛋白复合物的新特征 使用基于下一代测序的方法进行结构功能研究，(2) HyPR-MS、siRNA 和 sCAS9/SAM (CRISPR) 筛选，用于识别和验证用于结构功能研究的新蛋白质靶标，(3) 为生物学和结构重点项目提供病毒学和一般分子生物学支持 和目标，以及 (4) 计算机模拟、体外和细胞内 RNA 靶向筛选和化学合成以及 化学信息学/机器学习技术能够发现RNA靶向抑制剂 CRNA 中研究的生物过程。