Respiratory Effects, Metal and Aldehyde exposure from e-cigarette use in young adults (REMA)

年轻人使用电子烟对呼吸系统的影响、金属和醛的暴露 (REMA)

• 批准号: 10505765
• 负责人: Angela Aherrera
• 金额: $ 15.94万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10505765
• 关键词: Acrolein; Adult; Aerosols; Affect; Aldehydes; Behavior; Biological Markers; Blood; Characteristics; Chemical Exposure; Chemicals; Clinical; Complement; Cross-Sectional Studies; Data; Device Designs; Devices; Electronic cigarette; Environmental Epidemiology; Environmental Health; Epidemiologist; Epithelial Cells; Exhalation; Formulation; Free Radicals; Frequencies; Gene Expression; Goals; Health; Heating; High Pressure Liquid Chromatography; Immunologist; Individual; Inductively Coupled Plasma Mass Spectrometry; Inflammation; Inflammatory; Inhalation; Inorganic Chemicals; Isoprostanes; JUUL; Joints; Knowledge; Lung; Malondialdehyde; Marketing; Measurement; Measures; Mediating; Mediation; Mediator of activation protein; Mentors; Metal exposure; Metallothionein; Metals; Methods; Nasal Epithelium; National Institute of Environmental Health Sciences; Nature; Nicotine; Nitric Oxide; Nose; Organic Chemicals; Outcome; Oxidative Stress; Participant; Patient Recruitments; Phase; Positioning Attribute; Prevalence; Proteins; Proxy; Questionnaires; Regimen; Regulation; Research; Research Personnel; Respiratory Signs and Symptoms; Sampling; Scientist; Source; Spirometry; Stress; Symptoms; Tobacco; Toxic effect; Training; Urine; Work; aged; airway inflammation; bronchial epithelium; chemokine; cinnamic aldehyde; cohort; cytokine; design; e-cigarette aerosols; electronic cigarette use; electronic cigarette user; electronic liquid; improved; inflammatory marker; lung health; metal poisoning; non-smoker; novel; pollutant; pulmonary function; recruit; respiratory; respiratory health; skills; tobacco control; toxic metal; vaping; young adult

PROJECT SUMMARY My long-term goal is to become an independent investigator focusing on how pollutant exposures may adversely affect respiratory health and identify measures to effectively mitigate such exposures. My primary project objective is to investigate the exposure and toxicity of chemical constituents of concern (CCOC), namely metal and aldehydes, as well as the pulmonary health effects, including inflammation, of using new and emerging electronic cigarette (e-cig) devices among young adults. E-cig devices work by heating a mixture of chemicals to generate an aerosol that is inhaled by the user. Use of e-cigs has increased and, among adults, remains the highest among those aged 18 to 24 years. More recent e-cig devices such as disposable PODs have grown in popularity, yet it is currently unknown whether these new devices’ design characteristics in conjunction with user vaping regimen impact CCOC exposure and influence respiratory health. Thus, my specific aims are to 1) evaluate the relationship between e-cig use and CCOC exposure and effect, 2) assess the association of e-cig use with respiratory outcomes and inflammatory markers, and 3) assess CCOC exposure as a mixture and potential mediator in e-cig related respiratory health outcomes. In this cross-sectional study, to achieve Aim 1 (K99 phase), 150 participants (75 e-cig users, 75 non-users) will be recruited to assess biomarkers of exposure (aldehydes, metals) and effect (metallothionein) from e-cig use. This will leverage the ongoing EMIT study which looks at metal exposure and collects e-cig user regimen via questionnaire, aerosol samples, biospecimens (blood, urine), and spirometry measures. After receiving training in chemical analysis, respiratory clinical outcomes, and inflammatory markers, including gene expression changes, a new cohort of 150 participants (75 e-cig users, 75 non-users) will be recruited for Aim 2 (R00 phase). This phase will not only collect the same data as in Aim 1 but also biomarkers of effect and inflammation (blood, urine, FeNO) and gene expression profiles (in nasal epithelial cells). Whether e-cig users have increased respiratory symptoms, inflammation and altered gene expression profiles compared to non-users will be evaluated. Combining Aims 1 and 2 cohorts (n= 300), Aim 3 will employ the use of Bayesian and causal mediation methods to assess if CCOC exposure is positively associated with and explains, at least in part, the respiratory effects from e-cig use. With the proliferation of newer e-cig devices, there is an urgent need to characterize exposure and respiratory health effects resulting from their use. This study has the potential to generate critical data to inform FDA regulation to limit adverse exposures and health outcomes and curb the increasing prevalence of use among young adults. Through this research, my didactic coursework, and the guidance of my mentoring team consisting of a pulmonologist, exposure scientist, immunologist, analytical chemist, and environmental epidemiologist, I will acquire critical skills needed to be a successful independent researcher in environmental health and tobacco control.

项目摘要 我的长期目标是成为一名独立研究者，专注于污染物的暴露方式可能会对 影响呼吸健康并确定有效减轻此类暴露的措施。我的主要项目 目的是研究化学构成的暴露和毒性（CCOC），即金属 使用新的和新兴 年轻人中的电子烟（E-cig）设备。电子烟设备通过加热化学物质的混合物来起作用 生成用户继承的气溶胶。电子烟的使用增加了，在成年人中， 在18至24岁的人中最高。最近的电子烟设备（例如一次性豆荚）已经生长 流行，但是目前尚不清楚这些新设备的设计特性是否与用户结合 VAPing方案会影响CCOC暴露并影响呼吸健康。那是我的具体目的是1） 评估电子烟的使用与CCOC暴露与效果之间的关系，2）评估电子烟的关联 与呼吸结局和炎症标志物一起使用，3）将CCOC暴露作为混合物和 电子烟相关呼吸健康结果中的潜在介体。在这项横断面研究中，以实现目标1 （K99阶段），将招募150名参与者（75个E-cig用户，75名非用户）来评估曝光的生物标志物 （醛，金属）和效果（金属硫氨酸）。这将利用正在进行的发射研究 查看金属的曝光，并通过问卷调查，气雾剂样品，生物测量来收集E-cig用户方案 （血液，尿液）和肺活量测定。接受化学分析的培训后，呼吸道临床 结果和炎症标记，包括基因表达变化，新的150名参与者（75个） E-cig用户，75名非用户）将被招募为AIM 2（R00阶段）。此阶段不仅会收集相同的数据 如AIM 1一样，以及效应和影响的生物标志物（血液，尿液，Feno）和基因表达谱（在 鼻皮细胞）。电子烟使用者是否增加了呼吸道症状，感染和基因改变 将评估与非用户相比的表达曲线。组合目标1和2队列（n = 300），目标3 将使用贝叶斯和因果调解方法来评估CCOC是否积极暴露 至少部分地与E-CIG使用相关联并解释了呼吸作用。随着 较新的电子烟设备，迫切需要表征暴露和呼吸健康效应 从他们的使用中。这项研究有可能生成关键数据以告知FDA法规以限制对敌人 暴露和健康结果，并遏制年轻人使用率的日益增长的患病率。通过这个 研究，我的教学课程以及由肺科医生组成的心理团队的指导， 暴露科学家，免疫学家，分析化学家和环境流行病学家，我将获得关键 技能需要成为一名成功的环境健康和烟草控制方面的独立研究人员。