Accessible label-free optical microscopy with quantitative molecular and functional contrast

易于使用的无标记光学显微镜，具有定量分子和功能对比

• 批准号: 10501498
• 负责人: Francisco E Robles
• 金额: $ 36.75万
• 依托单位: GEORGIA INSTITUTE OF TECHNOLOGY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-21 至 2027-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10501498
• 关键词: Address; Award; Back; Biological; Biological Process; Biology; Cells; Classification; Complex; Computing Methodologies; Detection; Disease; Foundations; Functional Imaging; Future; Goals; Image; Imaging Device; Label; Laboratories; Lighting; Medicine; Methods; Microscopy; Molecular; Morphology; National Institute of General Medical Sciences; Optical Methods; Optics; Phase; Phenotype; Pump; Research; Research Personnel; Spectrum Analysis; Staging; System; Techniques; Technology; Vision; Work; base; cost; deep neural network; disease diagnosis; image translation; imaging modality; improved; innovation; molecular imaging; novel; optical imaging; programs; signal processing; software development; spectroscopic imaging; technology development; tool; ultraviolet

Project Summary/Abstract: Overview of research in the laboratory: The Optical Imaging and Spectroscopy (OIS) laboratory focuses in de- veloping and advancing optical technologies to help improve the understanding of biological processes and the ability to identify disease. Specifically, the OIS lab focuses on label-free imaging, linear and nonlinear spectros- copy, and advanced signal processing techniques to gain access to novel forms of functional and molecular in- formation for a variety of applications. To date, these efforts have led to advances in spectroscopic optical co- herence microscopy (SOCT), quantitative phase imaging (QPI), nonlinear pump-probe microscopy, and stimu- lated Raman scattering (SRS). The lab has pioneered several new forms of molecular contrast, including molec- ular reorientation, nonlinear phase dispersion spectroscopy, ultraviolet hyperspectral imaging, and dispersion- based SRS. The lab has also developed new computational methods for analyzing spectral and morphological features in an unsupervised manner. Finally, these methods have been applied to help in cell identification and phenotyping, basic biological studies, disease detection and staging, and more. Goals for the next five years, and the overall vision of the research program: Recently, the OIS lab pioneered quantitative oblique back illumination microscopy (qOBM) and has made important advances in deep ultravio- let (UV) microscopy as a means to address critical challenges in biology and medicine. Over the five years of this proposal, this program seeks to explore the capabilities of these highly promising new tools. Through a combination of system/hardware and computational/software developments, the proposed program will show the tremendous utility of these label-free optical methods to reveal critical molecular, functional, and structural detail that is currently impossible to obtain or requires prohibitive expensive or complex systems.. The main focus of the work is on technology development, but it will also explore a number of applications that are in dire need of such label-free, non-invasive, low-cost, easy-to-use imaging methods. The proposed program will lead to a robust platform that will guide future innovation. A clear example is tying in the tremendous power of emerging computational methods, such deep neural networks for image transla- tion and classification. Additionally, with the advent of the proposed tools and a better understanding of their capabilities, researchers will be able to venture into more targeted effort to tailor these tools for specific appli- cations. Thus, the proposed work and the capabilities it will enable in the future can have tremendous potential to improve and influence biology and medicine.

项目摘要/摘要： 实验室研究概况：光学成像和光谱（OIS）实验室重点研究 开发和推进光学技术，以帮助提高对生物过程和 识别疾病的能力。具体来说，OIS 实验室专注于无标记成像、线性和非线性光谱 复制和先进的信号处理技术，以获得新形式的功能和分子信息 形成用于各种应用。迄今为止，这些努力已经带来了光谱光学合作的进步 此处显微镜 (SOCT)、定量相位成像 (QPI)、非线性泵浦探针显微镜和刺激 延迟拉曼散射（SRS）。该实验室开创了几种新形式的分子对比，包括分子对比 光重定向、非线性相色散光谱、紫外高光谱成像和色散 基于SRS。该实验室还开发了用于分析光谱和形态的新计算方法 以无人监督的方式呈现特征。最后，这些方法已被应用于帮助细胞识别和 表型分析、基础生物学研究、疾病检测和分期等。 未来五年的目标以及研究计划的总体愿景：最近，OIS实验室开创了 定量斜背照显微镜（qOBM）在深紫外领域取得了重要进展 让（紫外）显微镜成为解决生物学和医学领域关键挑战的一种手段。五年来 根据该提案，该计划旨在探索这些极具前景的新工具的功能。通过一个 系统/硬件和计算/软件开发的结合，拟议的计划将显示 这些无标记光学方法在揭示关键分子、功能和结构方面的巨大效用 目前无法获得或需要昂贵或复杂的系统的详细信息。 工作重点是技术开发，但也会探索一些应用 迫切需要这种无标记、非侵入性、低成本、易于使用的成像方法。 拟议的计划将形成一个强大的平台，指导未来的创新。一个明显的例子是捆绑 在新兴计算方法的巨大力量中，这种用于图像翻译的深度神经网络 化和分类。此外，随着所提出的工具的出现以及对其的更好理解 能力，研究人员将能够投入更有针对性的努力，为特定应用定制这些工具 阳离子。因此，拟议的工作及其未来将实现的功能可能具有巨大的潜力 改善和影响生物学和医学。