Core B: Biological Models, Multiplexed Optical Biopsy, Molecular Pathology, and Biostatistics Core

核心 B：生物模型、多重光学活检、分子病理学和生物统计学核心

基本信息

批准号：
10494489
负责人：
Bryan Quilty Spring
金额：
$ 24.01万
依托单位：
MASSACHUSETTS GENERAL HOSPITAL
依托单位国家：
美国
项目类别：
财政年份：
1999
资助国家：
美国
起止时间：
1999-12-01 至 2027-08-31
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/10494489
关键词：
Animal Model Basal cell carcinoma Biochemical Biological Assay Biological Markers Biological Models Biometry Biopsy Biostatistics Core Cancer Model Clinic Clinical Communication Computer Analysis Computer software Cost Savings Custom Data Analyses Data Collection Development Distal Drug Delivery Systems Endoscopy Enzyme-Linked Immunosorbent Assay Experimental Designs Fluorescence Funding General Hospitals Gold Hand Histology Histopathology Human Resources Image Imaging technology Immune Immune checkpoint inhibitor Immune response Immunocompetent Immunohistochemistry In Situ In Vitro Infrastructure Institution Laboratories Location Lymphocyte Malignant neoplasm of pancreas Maps Massachusetts Methods Microscopy Modeling Monitor Neoplasm Metastasis Operative Surgical Procedures Optical Biopsy Organoids Pathologic Pathologist Pathology Patients Peripheral Pharmacotherapy Phenotype Population Pre-Clinical Model Primary Neoplasm Proteins Protocols documentation Quality Control Quality of life Reagent Regimen Reproducibility Research Research Personnel Residual Cancers Resources Services Skin Skin Cancer Specimen Statistical Data Interpretation System Techniques Technology Testing Therapeutic Time Tissues Toxic effect Treatment outcome Tumor Markers Tumor-infiltrating immune cells Universities Validation Visualization Work base biological development cancer cell cancer imaging cancer immunotherapy cell type central database checkpoint inhibition clinical translation cost data management data sharing design experience flexibility image guided improved in vivo in vivo Model in vivo imaging individualized medicine instrumentation lymph nodes medical schools microendoscope microendoscopy molecular pathology mouse model novel pancreatic cancer patients peripheral blood personalized medicine pre-clinical precision medicine programs research and development side effect specific biomarkers success therapy development three dimensional cell culture three-dimensional modeling tool treatment response tumor tumor-immune system interactions web site

项目摘要

ABSTRACT The Biological Models, Multiplexed Optical Biopsy, Molecular Pathology, and Biostatistics Core, Core B, integrates novel biological models with newly developed biochemical and imaging assay platforms to enable the development of the treatments envisioned in the Program Projects. The objective of Core B is to provide a service and research platform to increase the efficiency of microscopy, biostatistics and pathology techniques used by all four Program Projects. Core B will also coordinate centrally communicating biostatistics and pathological services to support experimental design and the interpretation of preclinical and clinical results. Collectively, the Core aims to develop new flexible and scalable technologies for skin and pancreatic cancer in partnership with the Program Projects to move the field further towards personalized and individualized therapies by informing the timing of immune checkpoint inhibition. This could have a tremendous impact with implications in the long run for customized, precision immune checkpoint inhibition treatments that maximize efficacy while reducing toxicity. This research and development component of Core B will provide novel cancer models and tumor imaging technologies for the advancement of the Program Projects. Core B is interactive with all of the Program Projects and each Project will leverage Core B resources and services, as follows: newly developed patient-derived in vitro organoid models from skin biopsies (Project 1) and pancreatic cancer discarded surgical tissue (Project 3); previously established in vivo immunocompetent mouse models of skin cancer (Project 1) and pancreatic cancer (Project 3); and, novel video multiplexed in vivo microendoscopy (Projects 1, 2, and 3) that was developed and validated during the previous funding cycle. In addition, the Core provides biostatistics and molecular pathology services to Projects 1, 2, and 3, with guidance from expert biostatisticians as well as pathologists with expertise in skin and pancreatic cancer. The strong infrastructure at MGH and the Mayo Clinic available at no costs makes this ambitious plan for Core B possible within the minimal resources of the P01. The microscopy support includes unique hardware and software, with rigorous validations, for hyperspectral multiplexing and this use of operational, custom-designed systems also introduces not only unique capabilities but also cost savings compared to commercial solutions. Collectively, Core B has the necessary infrastructure and personnel to maximize the success of the Program Projects in the discovery of novel combination regimens for photodynamic priming of immune checkpoint inhibition with potential to provide durable treatment outcomes and to improve quality of life for both skin and pancreatic cancer patients.

抽象的生物模型，多路复用光学活检，分子病理学和生物统计学核心，核心B，将新颖的生物学模型与新开发的生化和成像测定平台相结合，以启用计划项目中设想的治疗的发展。核心B的目的是提供服务和研究平台，以提高显微镜，生物统计学和病理技术的效率所有四个程序项目都使用。核心B还将在集中沟通生物统计学和支持实验设计的病理服务以及临床前和临床结果的解释。核心旨在为皮肤和胰腺癌开发新的灵活和可扩展技术与计划项目的合作伙伴关系，以进一步将领域朝向个性化和个性化通过通知免疫检查点抑制的时间来进行疗法。这可能会对从长远来看，对自定义的，精确的免疫检查点抑制作用的含义最大化降低毒性的功效。核心B的研究和开发部分将提供新的癌症用于进步计划项目的模型和肿瘤成像技术。核心B是互动的借助所有计划项目，每个项目都将利用核心B资源和服务，如下：来自皮肤活检（项目1）和胰腺癌的新开发的患者衍生的体外器官模型丢弃的手术组织（项目3）；先前在体内免疫能力的皮肤模型中建立的癌症（项目1）和胰腺癌（项目3）；而且，新型视频在体内微观镜检查（项目1、2和3）是在上一个资金周期中开发和验证的。另外，核心在专家的指导下，为项目1、2和3提供生物统计学和分子病理学服务生物统计学家以及具有皮肤和胰腺癌专业知识的病理学家。强大的基础设施 MGH和Mayo诊所无需任何费用，这使得核心B的雄心勃勃的计划成为可能 P01的最小资源。显微镜支持包括独特的硬件和软件，并具有严格的验证，用于高光谱多路复用以及对操作，自定义设计的系统的使用与商业解决方案相比，不仅引入了独特的功能，而且还节省了成本。共同核心B具有必要的基础架构和人员，以最大程度地提高计划项目的成功发现新型组合方案，用于与免疫检查点抑制的光动力启动提供持久的治疗结果并改善皮肤和胰腺的生活质量癌症患者。