Core B Bioinformatics Core

核心 B 生物信息学核心

• 批准号: 10494656
• 负责人: Shannan J Ho Sui
• 金额: $ 19.44万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-30 至 2027-05-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10494656
• 关键词: ATAC-seq; Achievement; Address; Bioinformatics; Biological Models; Chromatin; Clinical; Computer software; DNA Methylation; Data; Data Analyses; Data Management Resources; Data Set; Deposition; Epigenetic Process; Event; Experimental Designs; Experimental Models; Formulation; Functional disorder; Gene Expression; Genes; Genomic Segment; Goals; Infrastructure; Intuition; Investigation; Link; Maps; Metabolic; Methods; Molecular; Online Systems; Organism; Pathway interactions; Public Health Schools; Quality Control; Receptor Cell; Regulatory Pathway; Research; Research Personnel; Resources; Retrieval; Role; Sampling; Signal Transduction; Skin; Skin wound healing; Software Engineering; Supervision; System; Testing; Visualization; Visualization software; Work; age related; base; bioinformatics pipeline; data exploration; design; experimental study; insight; member; multidisciplinary; multiple omics; next generation sequencing; novel; programs; skin regeneration; statistics; stem cells; transcriptome sequencing; transcriptomics; web portal; wound healing

SUMMARY The Core B. Bioinformatics Core will serve as a comprehensive, multi-disciplinary resource of expertise in bioinformatics and statistics. It will support the data analysis needs of the program project, collaborating closely with investigators in the design, management, analysis and exploration of their high throughput omics experiments. Core B will assist investigators in addressing their aims at all levels to provide insights into age- related skin regeneration and wound healing through bioinformatics inference. The transcriptomics, DNA methylation and chromatin accessibility data generated through this program project will be consistently analyzed using state-of-the-art methods for next generation sequencing analysis implemented in Core B’s infrastructure that provide rigorous quality control, mapping, and quantitation of genes and genomic regions with functional roles in skin regeneration. Using established unsupervised and supervised multi-omics integrative approaches, Core B will identify shared signals across experiments and work with program investigators to map key metabolic, epigenetic and cell receptor stem cell pathways that drive age-related wound healing dysfunction. The results of these analyses will be made available through an interactive web based visualization system for storage, retrieval and exploration of the data generated by the program projects, enabling the achievement of the project aims and facilitating formulation of new testable hypotheses beyond those stated.

概括 核心 B. 生物信息学核心将作为一个全面的、多学科的专业资源 它将支持该计划项目的数据分析需求，并密切合作。 与研究人员一起设计、管理、分析和探索高通量组学 核心 B 实验将协助研究人员实现各个层面的目标，以提供对年龄的见解。 通过生物信息学推断相关的皮肤再生和伤口愈合。 将分析通过该计划项目一致生成的甲基化和染色质可及性数据 使用最先进的方法在 Core B 的基础设施中实施下一代测序分析 提供严格的质量控制、绘图和定量基因和基因组区域的功能 使用已建立的无监督和监督多组学综合方法， 核心 B 将识别实验中的共享信号，并与项目研究人员合作绘制关键代谢图谱， 表观遗传和细胞受体干细胞途径驱动与年龄相关的伤口愈合功能障碍。 这些分析将通过基于交互式网络的可视化系统进行存储、检索 和探索计划项目生成的数据，从而实现项目目标和 促进制定超出所陈述的新的可检验假设。