The Duke Senescent Cell Evaluations in Normal Tissues (SCENT) Mapping Center

杜克大学正常组织衰老细胞评估 (SCENT) 绘图中心

• 批准号: 10492746
• 负责人: ANDREW B. NIXON
• 金额: $ 238.1万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-30 至 2026-08-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10492746
• 关键词: Address; Age; Aging; Area; Atlases; Biochemical; Bioinformatics; Biological; Blood; Cell Aging; Cell model; Cells; Cellular Structures; Clinical; Clinical Trials; Colon; Communication; Computer software; Consultations; Critical Care; Data; Data Analyses; Data Collection; Development; Disease; Evaluation; Faculty; Future; Goals; Heart; Heart Transplantation; Heterogeneity; Human; Immune; Immunologics; Immunophenotyping; Improve Access; Institution; Investigation; Knowledge; Leadership; Lung; Lung Transplantation; Methods; Mission; Molecular; Muscle; Normal Cell; Normal tissue morphology; Organ; Organoids; Peripheral Blood Mononuclear Cell; Phase; Phenotype; Problem Solving; Process; Protocols documentation; Research; Resolution; Scientist; Skin; Specimen; Supervision; Testing; Therapeutic; Tissues; Translational Research; Transplantation Surgery; United States National Institutes of Health; Universities; age related; base; biobank; biological adaptation to stress; data integration; data sharing; deep learning algorithm; demographics; design; disability; healthy aging; human tissue; interoperability; medical schools; multimodality; normal aging; preservation; prospective; repaired; resilience; senescence; single cell technology; skills; targeted treatment; tissue mapping

ABSTRACT –SCENT– Overall Cellular senescence is a stress-response, as well as a critical component of cell fate during development, repair, resilience and normal aging. Deepening and broadening our investigations into cellular senescence in normal condition will advance our knowledge of healthy aging as well as age-related disabilities, thereby leading to integrated and inclusive approaches to Gero-Protection and Gero-Therapeutics. A comprehensive, high- resolution, Atlas of cellular senescence in various human tissues based on multimodal and multidimensional analyses is required to address this need. The Duke Senescent Cell Evaluations in Normal Tissues (SCENT) U54 Tissue Mapping Center (TMC) will leverage the broad and world-leading expertise of Duke University School of Medicine faculty to contribute to the overall goal of mapping senescent cells at single cell resolution in normal human tissues of across age-span and demographics. We will take advantage of Duke's broad expertise in many areas, including pulmonary critical care, lung and heart transplant surgery, translational research, and bioinformatics, to collect high-quality normal tissue specimens for biobanking, multimodal, high-resolution analysis, and data integration to fully characterize cellular senescence in human organs and tissues. The Duke SCENT TMC will be organized around 4 Cores as required by SenNet: The Administrative Core (AC) of this TCM will provide guidance and leadership to the other cores and communicate with NIH Staff and the SenNet Consortium during the set-up phase to agree on common protocols. The Biospecimen Core will establish best practices for prospective acquisition, preservation and processing of diverse, high-quality normal healthy human tissues and associated biofluids. The Biological Analysis Core will deliver state-of-the-art profiling, led by a team of highly accomplished research scientists who have successfully utilized these specific platforms in conjunction with previous and/or current senescent analyses. The Data Analysis Core (DAC) will manage, process and analyze the data generated to profile senescent cell signatures and construct senescent cell tissue maps, as well as collaborate with the Administrative Core to coordinate sharing of data, methods and pipelines with other SenNet institutions.

摘要 - 总体 - 总体 细胞衰老是一种响应 韧性和正常衰老。 条件将提高我们对健康年龄相关的残疾的了解，从而导致 综合和包容的方法，用于Gero保护和Gero-Thereapeics。 分辨率，基于多模式和多维的各种人体组织中细胞衰老的地图 需要分析以满足这种需求。 U54纸巾映射中心（TMC）将利用杜克大学学校的广阔领先专业知识 医学院的总体目标是在正常情况下以单细胞分辨率绘制衰老细胞 跨年龄和人口统计的人体组织。 区域，包括肺重症监护，肺和心脏移植手术，转化研究以及 生物信息学，收集高质量的正常组织标本，用于生物群，多模式，高分辨率 分析以及数据整合以完全表征人体器官和组织中的细胞衰老。 Duke Scent TMC将按照Sennet的要求组织约4个核心： 该TCM的行政核心（AC）为其他核心提供指导和领导 在设置阶段与NIH员工和Sennet联盟进行沟通，以同意共同的协议。 Biospecimen Core将建立前瞻性获取，保存和处理的最佳实践 多样化的高质量正常健康的人类组织和相关的生物流体。 生物分析核心将提供最新的分析，由一支高度成就的团队领导 成功地利用了这些特定平台以及以前/或 当前感觉分析。 数据分析核心（DAC）将管理，处理和分析生成的剖面衰老单元的数据 签名和构建衰老细胞组织图，以及与行政核心的合作。 协调数据，方法和管道的共享与其他SENNET结构。