The Development of Tabletop Brain-Mapping Platform

桌面脑图平台的开发

• 批准号: 10487512
• 负责人: Timothy M. Ragan
• 金额: $ 44.27万
• 依托单位: TISSUEVISION, INC.
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-09-10 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10487512
• 关键词: 3-Dimensional; Address; Agar; Anatomy; Area; Atlases; BRAIN initiative; Brain; Brain Mapping; Brain imaging; Brain region; Budgets; Central Nervous System Diseases; Client; Complex; Computer software; Development; Devices; Ensure; Equipment; Face; Feedback; Fluorescence; Foundations; Glass; Histology; Human Resources; Humidifier; Image; Immunohistochemistry; Individual; Institutes; Laboratories; Location; Manuals; Maps; Methods; Microscope; Microtome - medical device; Microtomy; Mus; Names; Nerve Degeneration; Neuraxis; Neurosciences; Organ; Pattern; Phase; Price; Process; Production; Protocols documentation; Research; Series; Slice; Slide; Societies; Software Tools; Stains; Surveys; System; Taxus; Techniques; Technology; Testing; Therapeutic; Thick; Thinness; Tissue imaging; Tissues; To specify; Work; base; cell type; commercialization; cost; cost effective; design and construction; digital; experience; image processing; improved; indexing; instrument; instrumentation; interest; multiphoton imaging; novel therapeutics; relating to nervous system; sample fixation; secondary analysis; software development; tissue preparation; tissue processing; tomography; tool; two-photon

PROJECT SUMMARY Central Nervous System (CNS) disorders place an enormous burden on society and have proven to be among the most challenging areas for which to develop a therapeutic. A major reason for this is that the brain is a complex, 3D, multiscale organ, with a heterogenous distribution of components across hundreds of brain regions. Extensive BRAIN Initiative efforts are underway to map this complexity by conducting surveys of connectivity pattern and cells types. Digital brain atlases such as the Allen Mouse Brain CCF v3.0 have become essential tools in organizing the results of these endeavors. The availability of the Allen CCFV3.0 and whole-brain surveys are transforming CNS studies. However, brain mapping still too frequently requires access to expensive equipment, specialized techniques, and dedicated personnel. Moreover, many newer techniques are incompatible with established histology protocols, which severely limits the contribution of foundational techniques to brain mapping efforts. In addition, the tedious manual production of slides remains a significant obstacle to high throughput histology. These difficulties present a significant barrier to the field and hinders progress in understanding the CNS. This proposal will remove these barriers by developing an affordable, table-top hardware and software mouse brain imaging and tissue processing platform accessible to the non-specialist neuroscience lab. It is based on the proven microtomy, section capture, and slide mounting technology contained in TissueVision's commercially successfully state-of-the-art multiphoton- based TissueCyte platform. This proposal will extend the capabilities of our current system and extend it to sections as thin as 15 microns and replace the TissueCyte's advanced multiphoton imaging with less expensive block-face imaging. In Phase II we will be package the integrated tissue processing technology into a convenient, compact instrument. This table-top, affordable device will combine the ease of working from 2D sections with the power of 3D brain analyses and help propel forward the development of new therapeutics. This product will be brought to market by a team of world-class experts at TissueVision who have successful commercialization experience in instrumentation for high throughput histology and brain mapping. We will be aided by a superb network of partners in leading neuroscience labs who will beta test the product and provide crucial feedback in Phase II to ensure a successful product launch and acceptance into the marketplace.

项目摘要 中枢神经系统（CNS）疾病给社会带来巨大负担，并已证明 成为开发治疗性的最具挑战性领域之一。主要原因 这是大脑是一个复杂的3D，多尺寸器官，具有异源分布 数百个大脑区域的组成部分。大脑倡议的努力正在进行中 通过进行连通模式和细胞类型的调查来绘制这种复杂性。数字的 诸如Allen Mouse Brain CCF v3.0之类的脑图已成为组织的重要工具 这些努力的结果。 Allen CCFV3.0和全脑调查的可用性正在改变中枢神经系统研究。 但是，大脑映射仍然经常需要使用昂贵的设备，专门 技术和敬业的人员。此外，许多较新的技术与 建立的组织学方案，严重限制了基础的贡献 大脑映射工作的技术。此外，幻灯片的乏味手动生产 仍然是高通量组织学的重要障碍。这些困难带来了 该领域的重大障碍并阻碍了理解中枢神经系统的进展。 该建议将通过开发负担得起的台式硬件和 软件鼠标脑成像和组织处理平台可访问非专家 神经科学实验室。它基于经过验证的显微镜切开术，截面捕获和滑动安装 Tissuevision商业上最先进的多光子 - 基于组织平台。该建议将扩展我们当前系统的功能，并 将其扩展到15微米的薄部分，然后更换组织的高级多光子 具有较便宜的块面成像成像。在第二阶段，我们将成为集成的包装 组织加工技术变成一种方便，紧凑的仪器。这个桌面，负担得起 设备将结合2D部分的易用性和3D脑分析的功率 并有助于推动新疗法的发展。 该产品将由Tissuevision的世界一流专家团队推向市场 在高通量组织学方面具有成功的商业化经验 和大脑映射。我们将得到领导神经科学领导的高级合作伙伴网络的帮助 将在II阶段测试产品并提供关键反馈的实验室，以确保 成功推出产品并接受市场。