Live Cell Fluorescent Assays for SARS-CoV-2 protease activity and COVID-19 Drug Discovery

SARS-CoV-2 蛋白酶活性和 COVID-19 药物发现的活细胞荧光测定

• 批准号: 10480515
• 负责人: THOMAS E HUGHES
• 金额: $ 30万
• 依托单位: MONTANA MOLECULAR, LLC
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-05-13 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10480515
• 关键词: 2019-nCoV; Biological; Biological Assay; Biosensor; COVID-19; COVID-19 assay; COVID-19 pandemic; COVID-19 therapeutics; COVID-19 treatment; Cells; Cellular Assay; Coronavirus; Development; Dimerization; Disease; Drug Screening; Enzymes; Fluorescence; Goals; Health; Hour; Human; Image; Immunohistochemistry; Intercalated Cell; Libraries; Liquid substance; Measurable; Measurement; Methods; Microscopy; Molecular; Montana; Noise; Nonstructural Protein; Peptide Hydrolases; Personal Satisfaction; Pharmaceutical Preparations; Phase; Protease Inhibitor; Proteins; Reader; Reporting; Research; SARS-CoV-2 genome; SARS-CoV-2 infection; SARS-CoV-2 protease; Signal Transduction; Site; Speed; Testing; Time; Translating; Viral; Virus; Virus Replication; assay development; cell type; combat; design; drug discovery; high throughput screening; inhibitor; lead optimization; prototype; response; screening; sensor; targeted treatment; tool; vector

SUMMARY STATEMENT The SARS-CoV-2 virus depends upon two proteases for replication, 3CLpro and PLpro. If one or both of these proteases can be blocked, COVID-19 infection can be effectively treated. Montana Molecular makes and sells live cell, fluorescent biosensor assays for disease research and drug discovery. In response to the COVID-19 pandemic, Montana Molecular applied its expertise and background in live cell assay development to build fluorescent biosensors for the SARS-CoV-2 main protease, 3CLpro. The prototype sensor described in this proposal demonstrates feasibility. Direct to Phase II support is requested here to speed the development of even brighter, more sensitive assays for the 3CLpro and PLpro proteases. These assays can be used in high throughput screening for compounds that inhibit the protease, and engage with their target in living, human cells. 406-539-7399 366 Gallatin Park Drive Suite A Bozeman, MT 59715

概要声明 SARS-CoV-2 病毒的复制依赖于两种蛋白酶：3CLpro 和 PLpro。如果可以阻断这些蛋白酶中的一种或两种，则可以阻止 COVID-19 感染 得到有效治疗。 Montana Molecular 生产和销售活细胞、荧光剂 用于疾病研究和药物发现的生物传感器测定。响应 COVID-19 大流行，蒙大拿分子应用其专业知识和背景 开发活细胞测定法来构建 SARS-CoV-2 荧光生物传感器 主要蛋白酶，3CLpro。本提案中描述的原型传感器 论证了可行性。此处请求直接提供第二阶段支持以加快 为 3CLpro 和 PLpro 开发更明亮、更灵敏的检测方法 蛋白酶。这些测定可用于化合物的高通量筛选 抑制蛋白酶，并与活的人类细胞中的目标结合。 406-539-7399 366 Gallatin Park Drive A 套房 博兹曼, MT 59715