Pathology Core

病理学核心

• 批准号: 10477224
• 负责人: James R Cook
• 金额: $ 14.48万
• 依托单位: MAYO CLINIC ARIZONA
• 依托单位国家: 美国
• 财政年份: 2018
• 资助国家: 美国
• 起止时间: 2018-09-19 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10477224
• 关键词: Accounting; Agreement; Ancillary Study; Benchmarking; Biological; Biological Assay; Case Study; Classification; Classification Scheme; Clinic; Clinical; Clonality; Consensus; Diagnosis; Diagnostic; Disease; Ensure; Evaluation; Fluorescent in Situ Hybridization; Follicular Lymphoma; Gold; Grant; Image; Immunohistochemistry; In Situ Hybridization; Individual; Institute of Medicine (U.S.); Institution; International; International Prognostic Index; Laboratories; Lymphoid; Lymphoma; Mantle Cell Lymphoma; Molecular Diagnosis; Molecular Diagnostic Testing; Molecular Genetics; Molecular Profiling; Morphology; Non-Hodgkin' s Lymphoma; Online Systems; Paraffin; Pathologic; Pathologist; Pathology; Pathology Report; Patient-Focused Outcomes; Patients; Phenotype; Prognosis; Public Health; Reporting; Research; Research Project Grants; Resources; Sampling; Sampling Studies; Scanning; Site; Slide; Southwest Oncology Group; Stains; System; Techniques; Testing; Tissue Microarray; Utilization Review; World Health Organization; accurate diagnosis; base; cooking; cost efficient; diagnosis standard; diagnostic accuracy; digital; digital imaging; experience; health goals; imaging platform; imaging system; leukemia/lymphoma; member; new technology; prognostic index; success; therapeutic target; therapy resistant; web based interface; whole slide imaging

The Pathology Core (Dr. Cook) will provide thorough central pathology review of all patient samples studied in Projects 1-4 of this grant, utilizing web-based e-pathology resources and providing additional ancillary testing analyses when required to arrive at a consensus diagnosis. Tissue microarrays will also be constructed from paraffin blocks for Projects 2 and 3, facilitating cost-efficient downstream analysis. Aim 1) To provide a system for central pathology review utilizing digital slide imaging. Central review by a panel of internationally recognized lymphoma experts has been one of the cornerstones of prior LLMPP studies. Each case is reviewed by four lymphoma pathology experts, and agreement by at least three pathologists defines a consensus diagnosis. For cases where an initial consensus is not reached, the cases are reviewed by the entire pathology committee together and a consensus diagnosis established by majority opinion. Web based e-pathology resources based in the Cleveland Clinic e-pathology center will be utilized by the Pathology Core to facilitate central review in a cost efficient manner. Routine sections and immunohistochemical stains will be scanned using an Aperio whole slide imaging system, and reviewing pathologists will be provided access to review their assigned cases through a web based interface. Aim 2) To complete ancillary studies as required to reach a final pathologic diagnosis. Additional ancillary testing may include immunohistochemical stains, fluorescence in situ hybridization (FISH) studies and/or lymphoid clonality testing, may be necessary in some cases to arrive at a definitive consensus diagnosis. Such testing, when requested by the pathologists performing central review, will be performed in the Cleveland Clinic research core laboratories which include 1 Ventana Discovery, 3 Ventana Benchmark XT, 2 Ventana Benchmark Ultra, and 1 Dako immunohistochemistry platforms. An extensive FISH menu and PCR clonality assays employing gold standard Euroclonality primer sets are also available. Aim 3) To facilitate individual research projects through construction of tissue microarrays. Tissue microarrays will be constructed from samples submitted for Projects 2 and 3. Dr. James Cook will serve as PI for the Pathology Core. Dr. Cook has extensive experience in central pathology review for multi-institutional studies, having been a member of the LLMPP lymphoma pathology committee and the SWOG lymphoma pathology committee since 2005. Dr. Cook is supported by a strong Research core group within the Cleveland Clinic Pathology and Laboratory Medicine Institute, including research technologists, project managers, accounting experts, and other administrative support. This Core will impact each of the Projects 1-4 in this grant by ensuring each case studied has been thoroughly vetted and providing a gold standard, consensus pathologic diagnosis

病理学核心（库克博士）将对所有研究的患者样本进行彻底的中央病理学审查 该赠款的项目 1-4，利用基于网络的电子病理学资源并提供额外的辅助测试 需要时进行分析以达成共识诊断。组织微阵列也将由 项目 2 和 3 的石蜡块，促进具有成本效益的下游分析。目标1）提供一个系统 利用数字幻灯片成像进行中央病理学检查。由国际专家小组进行集中审查 公认的淋巴瘤专家一直是先前 LMPP 研究的基石之一。每个案例都是 由四位淋巴瘤病理学专家审查，并得到至少三位病理学家的同意，定义了 共识诊断。对于未达成初步共识的案件，由委员会审查 整个病理学委员会一起并根据多数意见建立共识诊断。基于网络 病理核心将利用克利夫兰诊所电子病理中心的电子病理资源 以具有成本效益的方式促进中央审查。常规切片和免疫组化染色 使用 Aperio 全玻片成像系统进行扫描，并为审查病理学家提供访问权限 通过基于网络的界面查看分配给他们的案例。目标 2) 按要求完成辅助研究 以达到最终的病理诊断。其他辅助测试可能包括免疫组织化学染色、 荧光原位杂交（FISH）研究和/或淋巴克隆测试，在某些情况下可能是必要的 病例得出明确的共识诊断。当病理学家要求时进行此类测试 进行中央审查，将在克利夫兰诊所研究核心实验室进行，其中包括 1 Ventana Discovery、3 个 Ventana Benchmark XT、2 个 Ventana Benchmark Ultra 和 1 个 Dako 免疫组织化学平台。广泛的 FISH 菜单和采用金标准的 PCR 克隆分析 还提供 Euroclolonality 引物组。目标 3) 通过以下方式促进个人研究项目 组织微阵列的构建。组织微阵列将根据项目提交的样本构建 2 和 3。James Cook 博士将担任病理学核心的 PI。库克博士在以下方面拥有丰富的经验： 多机构研究的中央病理学审查，曾是 LLMPP 淋巴瘤的成员 自 2005 年起担任病理学委员会和 SWOG 淋巴瘤病理学委员会。库克博士得到了 克利夫兰诊所病理学和实验医学研究所内强大的研究核心小组，包括 研究技术专家、项目经理、会计专家和其他行政支持。该核心将 确保每个研究案例都经过彻底审查，并影响本次拨款中的每个项目 1-4 提供金标准、一致的病理诊断