Impact of Sleep Duration on Immune Balance in Urban Children with Asthma

睡眠时间对城市哮喘儿童免疫平衡的影响

• 批准号: 10468943
• 负责人: Daphne Koinis Mitchell
• 金额: $ 73.39万
• 依托单位: RHODE ISLAND HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-08-15 至 2026-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10468943
• 关键词: Address; Adult; Age; Allergic; Asthma; Beds; Biological Process; Chronic; Clinical; Data; Development; Disease; Enrollment; Environment; Equilibrium; Experimental Designs; Extrinsic asthma; Guidelines; Home; Immune; Immunologic Markers; Individual Differences; Inflammatory; Interferon Type II; Interferons; Interleukin-13; Interleukin-4; Interleukin-5; Interleukin-6; Interleukins; Intervention; Knowledge; Link; Modeling; Morbidity - disease rate; Participant; Plasma; Play; Polysomnography; Population; Protocols documentation; Pulmonary Function Test/Forced Expiratory Volume 1; Recovery; Research; Risk; Safety; Sampling; Schedule; Sleep; Sleep Deprivation; Spirometry; Stress; Symptoms; Testing; Time; Work; actigraphy; adequate sleep; clinical care; cytokine; experience; experimental study; high risk; high risk population; immune function; improved; improvement on sleep; modifiable behavior; neglect; novel; poor sleep; pulmonary function; sleep health; urban children; urban setting

Project Summary / Abstract Urban children with asthma are at high risk for short sleep, due to an environment that jeopardizes sleep and asthma management. Further, this group suffers from altered immune balance, a key biological process contributing to individual differences in asthma morbidity and sleep health. Allergic asthma is a chronic inflammatory disorder driven primarily by disturbed T helper 1 (Th1)/ 2 (Th2) cytokine balance marked by Th2 cytokine (IL-4, IL-5 and/or IL-13) predominance. Experimental findings in healthy adults show that shortened sleep increases inflammatory cytokine (e.g., IL-6) and certain Th2 cytokine levels and that recovery sleep following sleep restriction promotes a return to immune balance. Whether sleep duration plays a key role in immune function and associated asthma activity in urban children with asthma remains a scientific gap. We will use an experimental design that targets sleep duration, because (1) the urban environment and asthma symptoms interact to shorten sleep, (2) sleep duration is a modifiable behavior overlooked in clinical care of urban children with asthma, and (3) experimental data are critical to test a causal link for sleep duration as a mechanism underlying immune balance and asthma. We will enroll urban children (N=204; ages 8-9 years) with persistent allergic asthma and adequate sleep duration (9-11 h) who will complete a 4-week within-subjects protocol that includes 3 scheduled experimental sleep conditions: (1) 1 week stabilized sleep (individualized; 9-11 h time in bed), (2) 1 week shortened sleep (1.5 h decrease in time in bed), and (3) 2 weeks recovery sleep (1.5 h increase in time in bed). We will monitor sleep duration (actigraphy) and lung function (home spirometry) daily and assess immune biomarkers weekly and at the midpoint of shortened sleep. To control time-in-study effects, 1/3 of our sample will receive only the stabilized sleep schedule across the 4-week protocol. In this project, we will study only urban children with allergic asthma who obtain sufficient sleep (9-11 h, within national guidelines). Our shortened sleep protocol will model the sleep loss that urban children with asthma can experience due to asthma and/or urban context. Additionally, our recovery sleep protocol simulates a sleep optimization intervention following shortened sleep in a well-controlled approach. The first aim of the study is to examine the effects of shortened sleep on immune balance [e.g., Th1 (Interferon-IFNγ)/Th2 (Interleukin-IL-4, IL-5, IL-13)R and plasma IL-6 levels]. The second aim involves determining the effects of recovery sleep on immune balance. The third aim involves examining the extent to which changes in immune balance are associated with changes in asthma-related lung function (changes in FEV1) under conditions of shortened and recovery sleep. Results from this study ultimately will support the development feasible, ecologically valid, and clinically meaningful interventions to optimize sleep duration, immune balance, and asthma in this at-risk group.

项目概要/摘要 由于环境不利于睡眠和睡眠，城市患有哮喘的儿童睡眠不足的风险很高。 此外，该群体患有免疫平衡改变，这是一个关键的生物过程。 过敏性哮喘是一种慢性疾病，导致哮喘发病率和睡眠健康存在个体差异。 主要由辅助 T 1 (Th1)/ 2 (Th2) 细胞因子平衡紊乱（以 Th2 为标志）驱动的炎症性疾病 健康成人的实验结果表明，细胞因子（IL-4、IL-5 和/或 IL-13）优势缩短。 睡眠会增加炎症细胞因子（例如 IL-6）和某些 Th2 细胞因子水平，并且有助于恢复睡眠 睡眠限制后是否能促进免疫平衡的恢复。 城市哮喘儿童的免疫功能和相关哮喘活动仍然存在科学差距。 使用以睡眠时间为目标的实验设计，因为 (1) 城市环境和哮喘 症状相互作用会缩短睡眠，（2）睡眠持续时间是一种可改变的行为，在临床护理中被忽视 患有哮喘的城市儿童，以及（3）实验数据对于测试睡眠时间与睡眠时间之间的因果关系至关重要 免疫平衡和哮喘的潜在机制。 我们将招募患有持续性过敏性哮喘且睡眠充足的城市儿童（N=204；年龄8-9岁） 持续时间（9-11 小时），谁将完成为期 4 周的受试者内协议，其中包括 3 个预定的实验 睡眠条件：(1) 1 周稳定睡眠（个性化；9-11 小时卧床时间），(2) 1 周缩短睡眠 （卧床时间减少 1.5 小时），以及 (3) 2 周恢复睡眠（卧床时间增加 1.5 小时）。 每天测量睡眠时间（体动记录仪）和肺功能（家庭肺活量测定），并每周评估免疫生物标志物 在缩短睡眠的中点，为了控制研究时间的影响，我们的样本中 1/3 将仅接受 在整个 4 周的方案中，我们将只研究城市儿童的睡眠时间表。 过敏性哮喘患者获得充足的睡眠（9-11 小时，符合国家指南）。 将模拟城市哮喘儿童因哮喘和/或城市环境而经历的睡眠不足。 此外，我们的恢复睡眠方案模拟了睡眠时间缩短后的睡眠优化干预 以一种良好控制的方法。 该研究的首要目的是检查睡眠时间缩短对免疫平衡的影响[例如，Th1 （干扰素-IFNγ）/Th2（白细胞介素-IL-4、IL-5、IL-13）R 和血浆IL-6 水平]。 确定恢复睡眠对免疫平衡的影响。 免疫平衡的变化与哮喘相关肺功能的变化有关（ FEV1）在缩短和恢复睡眠的情况下，本研究的结果最终将支持这一观点。 开发可行的、生态有效的、具有临床意义的干预措施来优化睡眠时间， 该高危人群的免疫平衡和哮喘。