Role of TET1 in germ cell reprogramming and development
TET1 在生殖细胞重编程和发育中的作用
基本信息
- 批准号:10467364
- 负责人:
- 金额:$ 30.4万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2022
- 资助国家:美国
- 起止时间:2022-09-01 至 2026-06-30
- 项目状态:未结题
- 来源:
- 关键词:AddressAdultAgingAllelesBase Excision RepairsBehavioral AssayBiological AssayBrainChromatinCoupledDNADNA MethylationDNA Modification MethylasesDNA Modification ProcessDNA biosynthesisDataDevelopmentDiseaseEmbryoEngineeringEnsureEnzymesEpigenetic ProcessExcisionFailureFamilyFertilizationFibroblastsGenerationsGenesGeneticGenetic TranscriptionGenomeGenomicsGerm CellsGonadal structureHeterogeneityInfertilityLeadLinkMaintenanceMediatingMeiosisMethylationMouse StrainsMusMutant Strains MiceMutationOocytesOxidesPartner in relationshipPathway interactionsPatternPhenotypePlayProductionPublishingRoleShapesSomatic CellStructure of primordial sex cellSyndromeTechnologyTestingTissuesWild Type MouseWorkbasebead chipchromatin proteindemethylationdevelopmental diseaseeggepigenomeepigenomicsfetalgenome-widegenomic locushistone modificationimprintin vivoinduced pluripotent stem cellmammalian genomemethylation patternmouse genomemutantneurobehaviornew technologynext generationoffspringoxidationpreventpupresponsesexual dimorphismsperm cellsperm viabilitytooltranscriptome sequencingtransmission process
项目摘要
The mammalian germline must be reprogrammed to facilitate proper development. This reprogramming, which
includes the erasure of DNA methylation and histone modifications, ensures the establishment of gamete-
appropriate epigenetic patterns and minimizes the transmission of epimutations to offspring. While much of the
genome undergoes replication-coupled passive DNA demethylation, a critical role for Ten-eleven Translocation
(TET) family enzymes, specifically TET1, has been demonstrated for active demethylation of genomic
sequences such as imprinting control regions (ICRs) and germline-specific genes. The proposed work will use
newly developed mouse strains and sequencing technologies to test the hypothesis that iterative oxidation and
noncatalytic functions of TET1 are required for DNA methylation erasure and reprogramming of the mouse
genome, including ICRs and meiosis-specific genes, during germline and somatic development. TET enzymes
can catalyze up to three successive oxidations of 5-methylcytosine (5mC), generating 5-hydroxymethylcytosine
(5hmC), 5-formylcytosine (5fC), or 5-carboxycytosine (5caC). Oxidized 5mC bases, particularly 5hmC, can
play independent epigenetic roles in somatic tissues including the brain, but are most significantly thought to
function as DNA demethylation intermediates. The distinctive demethylation pathways supported by 5hmC
versus 5fC/5caC have confounded efforts to decipher the precise mechanistic role for TET1. Yet further
challenges are posed by potential non-catalytic roles for TET1, which is known to interact with chromatin
modifying enzymes. Published work and our preliminary data suggest that a role for catalytic and non-catalytic
TET1 activities for demethylation, but the mechanism, timing and target sequences remain incompletely
understood. Thus, we propose to address (1) whether iterative oxidation to 5fC/5caC is required for
reprogramming, (2) whether TET1 has a noncatalytic reprogramming role, and (3) what sequences require
various TET activities. Specific Aim 1 will examine the precise role of TET1 in reprogramming at ICRs and
genome-wide in primordial germ cells (PGCs). We have engineered mice that either stall 5mC oxidation at
5hmC (Tet1v) or lack catalytic function (Tet1hxd) and will test their effects on DNA methylation reprogramming
using our new technology which resolves 5mC and 5hmC, and profile associated chromatin dynamics during
PGC development. Our preliminary data using the new Infinium Mouse BeadChip suggest that the Tet1 mutant
mice sperm have non-overlapping aberrant patterns of DNA modification. Thus, Specific Aim 2 will assess the
epigenomic and phenotypic consequences of Tet1 mutations in homozygous mutant gametes and the offspring
that arise from these gametes. Finally, Specific Aim 3 will determine the epigenomic and phenotypic
consequences of Tet1 stalling and catalytic mutations in homozygous mutant adult and aging mice. This work
will enable an assessment of the role of TET enzymes in genome reprogramming, dissecting the requirement
of noncatalytic activity and iterative oxidation by TET enzymes.
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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MARISA S. BARTOLOMEI其他文献
MARISA S. BARTOLOMEI的其他文献
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{{ truncateString('MARISA S. BARTOLOMEI', 18)}}的其他基金
Role of TET1 in germ cell reprogramming and development
TET1 在生殖细胞重编程和发育中的作用
- 批准号:
10689734 - 财政年份:2022
- 资助金额:
$ 30.4万 - 项目类别:
Tri-Institutional Symposium on Reproductive Biology & Infertility (Tri-Repro)
生殖生物学三机构研讨会
- 批准号:
10171876 - 财政年份:2020
- 资助金额:
$ 30.4万 - 项目类别:
Tri-Institutional Symposium on Reproductive Biology & Infertility (Tri-Repro)
生殖生物学三机构研讨会
- 批准号:
10405090 - 财政年份:2020
- 资助金额:
$ 30.4万 - 项目类别:
Tri-Institutional Symposium on Reproductive Biology & Infertility (Tri-Repro)
生殖生物学三机构研讨会
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10626897 - 财政年份:2020
- 资助金额:
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