American and Asian Centers for Arboviral Research and Enhanced Surveillance (A2CARES)

美国和亚洲虫媒病毒研究和加强监测中心 (A2CARES)

基本信息

批准号：
10463114
负责人：
Josefina Coloma
金额：
$ 17.56万
依托单位：
UNIVERSITY OF CALIFORNIA BERKELEY
依托单位国家：
美国
项目类别：
财政年份：
2021
资助国家：
美国
起止时间：
2021-08-20 至 2022-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10463114
关键词：
Address Africa American Americas Arbovirus Infections Arboviruses Asia Asian Americans Asians Basic Science Chikungunya virus Clinical Clinical Data Clinical Management Clinical Research Cohort Studies Collaborations Communicable Diseases Communities Comparative Study Dengue Dengue Virus Detection Diagnosis Diagnostic Diagnostic Procedure Diagnostic tests Disease Disease Outbreaks Ebola Ecuador Emerging Communicable Diseases Epidemic Epidemiology Evaluation Family Foundations Future Geographic Locations Geography Goals Health Hospitals Human Immunology In Situ Incidence Individual Infection Infectious Diseases Research Infrastructure Interdisciplinary Study International Investigation Joints Knowledge Laboratories Latin America Location Longitudinal cohort Measures Methods Molecular Molecular Epidemiology National Institute of Allergy and Infectious Disease Nicaragua Orthobunyavirus Outcome Pattern Performance Phylogenetic Analysis Publications Readiness Research Research Infrastructure Research Personnel Rural Rural Population Sampling Serology Serology test Severities Severity of illness Site Specimen Sri Lanka Standardization Statistical Data Interpretation Sum Surveys System Testing Time Training Programs Vaccines Viral Viral Genome ZIKA Zika Virus age group authority base chikungunya cohort data management design emerging pathogen experience flexibility human pathogen innovation member method development multiplex assay next generation sequencing nonhuman primate novel novel diagnostics pandemic disease pathogen pathogenic virus peri-urban programs response surveillance data sustainability framework tool transmission process vaccine development vector

项目摘要

ABSTRACT Recent pandemics of human pathogens have revealed the limitations of current surveillance systems. The sequential arboviral epidemics in the Americas showed how outbreak detection can be delayed and opportunities missed to collect well-characterized specimens and data for surveillance, basic science, clinical research, and development of vaccines and new diagnostics. In response, we have assembled a consortium of world-renowned investigators in arbovirology, epidemiology, immunology, viral diagnostics, phylogenetics, and clinical research, while leveraging research infrastructure and expertise in long-term cohort and hospital-based studies. This has resulted from 3 decades of collaborative international research, with over 200 joint publications, and extensive experience in preparing for and responding to outbreaks working closely with local and international health authorities and NIAID. With key sites in Asia and the Americas, we will use innovative molecular and serological methods to identify emerging pathogens and to address fundamental questions in dengue and other arboviral epidemiology and test viral, host and environmental determinants of differences between sites along a gradient of urbanicity. Our overarching goal is to develop an interconnected, harmonized network of clinical and laboratory sites to strengthen research programs, compare disease epidemiology and severity in different regions, develop and implement cutting-edge diagnostic methods, and respond efficiently and effectively to outbreaks. Standardized hospital studies and community-based cohorts in Ecuador, Nicaragua and Sri Lanka will characterize and compare human arboviral illnesses across urban, peri-urban and rural sites and develop sustainable infrastructure to rapidly respond to epidemics together with Ministries of Health, comple- mented by outbreak investigation, surveillance of non-human primates, and vector incrimination. We will imple- ment standardized plans for study administration, data and clinical management, and statistical analysis across sites, supported by an extensive on-site training program. Aim 1 will establish standard and novel multiplex assays at each site for surveillance, diagnosis and research of arboviruses and other pathogens. Aim 2 com- pares dengue/arbovirus epidemiology and transmission in cohort studies located in ecologically distinct regions over a continuum of urbanicity. In Aim 3, we will characterize complete viral genome sequences for comparative studies of arboviral phylogenetics, phylogeography and molecular epidemiology. Aim 4 consists of surveillance, identification, and characterization of novel and unrecognized human pathogens from severe hospitalized cases, unexpected outbreaks, vectors and non-human primates. Molecular and serological assays will be developed for newly identified pathogens and quickly implemented across study sites to characterize the epidemiology and clinical manifestations and will be made available to the EIDRC network. In sum, our A2CARES Consortium will provide valuable new tools and knowledge and 3 interconnected centers in Latin America and South Asia that will be able to respond to outbreaks and to study emerging and endemic infectious diseases far into the future.

抽象的最近的人类病原体大流行揭示了当前监测系统的局限性。这美洲连续发生的虫媒病毒流行病表明如何延迟疫情检测和机会错过了收集特征明确的标本和数据以用于监测、基础科学、临床研究和疫苗和新诊断方法的开发。作为回应，我们组建了一个由世界知名企业组成的联盟虫媒病毒学、流行病学、免疫学、病毒诊断、系统发育学和临床研究的研究人员，同时利用长期队列和医院研究中的研究基础设施和专业知识。这有是 3 年来国际合作研究的成果，已发表 200 多篇联合出版物，并广泛与当地和国际卫生部门密切合作准备和应对疫情的经验当局和 NIAID。我们将在亚洲和美洲的关键地点使用创新的分子和血清学技术识别新出现的病原体并解决登革热和其他虫媒病毒的基本问题的方法流行病学并测试沿梯度位点之间差异的病毒、宿主和环境决定因素的都市性。我们的首要目标是开发一个相互关联、协调一致的临床和实验室地点加强研究计划，比较疾病流行病学和严重程度不同地区，开发和实施尖端诊断方法，并高效应对有效地控制疫情的爆发。厄瓜多尔、尼加拉瓜的标准化医院研究和社区队列研究斯里兰卡将描述和比较城市、城郊和农村地区的人类虫媒病毒疾病并与卫生部一起开发可持续的基础设施，以快速应对流行病，完成以疫情调查、非人类灵长类动物监测和病媒归罪为指导。我们将实施—— 研究管理、数据和临床管理以及统计分析的标准化计划现场，并有广泛的现场培训计划支持。目标 1 将建立标准和新颖的多路复用在每个地点进行检测，以监测、诊断和研究虫媒病毒和其他病原体。目标 2 com- 位于不同生态区域的队列研究中的登革热/虫媒病毒流行病学和传播城市化的连续体。在目标 3 中，我们将表征完整的病毒基因组序列以进行比较虫媒病毒系统发生学、系统发育地理学和分子流行病学研究。目标 4 包括监视、严重住院病例中新型和未被识别的人类病原体的鉴定和特征分析，意外爆发、媒介和非人类灵长类动物。将开发分子和血清学检测针对新发现的病原体，并在各个研究地点快速实施，以描述流行病学特征和临床表现并将提供给 EIDRC 网络。总之，我们的 A2CARES 联盟将提供宝贵的新工具和知识，以及位于拉丁美洲和南亚的 3 个相互关联的中心将能够在遥远的未来应对疫情暴发并研究新出现的地方性传染病。