Cellular Components of Human Milk: An Examination of Their Role in Infant Health and Development and the Functional Impact of Hospital Storage Practices

母乳的细胞成分：检查其在婴儿健康和发育中的作用以及医院储存实践的功能影响

• 批准号: 10456969
• 负责人: Carrie-Ellen Briere
• 金额: $ 14.62万
• 依托单位: UNIVERSITY OF MASSACHUSETTS AMHERST
• 依托单位国家: 美国
• 财政年份: 2021
• 资助国家: 美国
• 起止时间: 2021-07-26 至 2026-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10456969
• 关键词: Address; Admission activity; Affect; Animal Model; Area; Biological; Birth; Brain; Bronchopulmonary Dysplasia; Cell Death; Cell physiology; Cells; Characteristics; Clinical; Clinical Research; Development; Ensure; Epithelial Cell Proliferation; Face; Fostering; Freezing; Future; Gastrointestinal tract structure; Genes; Goals; Growth; Growth and Development function; Health; Heart; Hospitals; Human Milk; Immune response; Immunologics; Impairment; Infant; Infant Development; Infant Health; Ingestion; Intervention; Intestines; Knowledge; Laboratories; Lactation; Life; Ligands; Lung; Methods; Milk; Modeling; Mothers; Necrotizing Enterocolitis; Neonatal; Neonatal Intensive Care Units; Neurological outcome; Newborn Infant; Organ; Outcome; Physiological; Play; Population; Premature Birth; Premature Infant; Property; Refrigeration; Research; Research Project Grants; Research Proposals; Research Support; Research Technics; Research Training; Risk; Role; Science; Severities; Supporting Cell; Technical Expertise; Training; Translational Research; Travel; Work; biological systems; body system; career; cell type; cytokine; donor milk; feeding; improved; innovation; interest; intestinal epithelium; laboratory experience; late onset sepsis; mammary; meetings; microbial; milk expression; mouse model; neonatal care; organ growth; pasteurization; personalized medicine; pluripotency; premature; programs; protective effect; regenerative; stem; stem cells; stem-like cell; tissue culture

Project Summary/Abstract Preterm infants are at increased risk of health complications due to their under-developed organ systems at birth, and human milk receipt is recognized as an important intervention to promote infant health. Recent identification of cells in human milk which have similar characteristics to stem cells, as well as animal models which show integration of these cells into nursling's organs, indicate an important function of milk cells which is currently not understood. In the NICU, infants often receive human milk which has been refrigerated or frozen, and then warmed and/or thawed prior to feeding. The impact of these typical handling practices on the cellular components of milk that may provide an important mechanism of biologic protection in infant health has not been investigated. This work will determine how the real-life storage and handling of human milk impacts infant health and development, and is the first to evaluate protective mechanisms. There are currently two major knowledge gaps in the fields of human milk and lactation and neonatal care surrounding bioactive cellular components. The first is a lack of knowledge of how real-life storage and handling practices impact the protective ability of milk's cellular components for infants. Without this knowledge, infants who may benefit from milk with the most bioactivity (such as preterm infants) may not receive the properties of their mother's milk which provide important protection. The second major opportunity is the lack of knowledge on the mechanism(s) of protection in milk stem-like cells. Additionally, as research techniques advance our ability to investigate milk components, there is a great need to look at milk components in a biological systems perspective. The specific aims of the research project are to 1) examine how hospital storage practices (refrigeration and freezing) impact the protective mechanisms of human milk cells through the use of a tissue culture model of intestinal health, and 2) determine if milk cells are integrated as functioning cells specific to vital organs impacted by preterm birth (brain, heart, lungs, intestine) using a cross-foster mouse model. Upon completion of the specific aims of the K23 research strategy and training plans, the candidate will have advanced theoretical knowledge and technical skills to conduct human milk research with the ability to apply a biological systems approach to understand the complexity of the many components of milk which likely impact the function of each. The proposal research aims are supported by research training, didactic coursework, scientific meetings, and specific plans for dissemination and future growth. Ultimately, the short- and long-term goals of this research are to 1) improve infant health by optimizing the delivery of the most bioactive human milk components and 2) determine the mechanism(s) by which human milk cells protect infants and promotes growth and development.

项目摘要/摘要 早产婴儿由于其​​欠发达器官系统的健康并发症的风险增加 出生，人乳收入被认为是促进婴儿健康的重要干预措施。最近的 鉴定具有与干细胞具有相似特征的人乳中的细胞以及动物模型 显示这些细胞整合到护士器官中的，这表明牛奶细胞的重要功能是 目前尚不理解。在NICU中，婴儿经常收到已冷藏或冷冻的人牛奶， 然后在进食之前加热和/或解冻。这些典型的处理实践对蜂窝的影响 牛奶的组成部分可能提供婴儿健康中生物保护的重要机制尚未 被调查。这项工作将确定现实生活中的储存和处理人类牛奶如何影响婴儿 健康和发展，是第一个评估保护机制的人。目前有两个主要 人乳和泌乳领域的知识差距以及围绕生物活性细胞的新生儿护理 成分。首先是缺乏了解现实生活中的存储和处理实践如何影响 牛奶为婴儿的细胞成分的保护能力。没有这些知识，可能会从中受益的婴儿 生物活性最多的牛奶（例如早产儿）可能无法收到母亲的牛奶的特性 提供重要的保护。第二个主要机会是缺乏有关 牛奶干细胞保护的机理。此外，随着研究技术提高了我们的能力 研究牛奶成分，非常需要查看生物系统中的牛奶成分 看法。研究项目的具体目的是1）检查医院存储实践如何 （制冷和冷冻）通过使用组织影响人奶细胞的保护机制 肠道健康的培养模型，以及2）确定牛奶细胞是否作为特定于功能细胞的功能细胞集成 受早产（大脑，心脏，肺，肠）影响的重要器官使用跨副小鼠模型。之上 完成K23研究策略和培训计划的具体目标，候选人将拥有 先进的理论知识和技术技能，以进行人性乳研究，并能够应用 生物系统方法了解牛奶的许多组成部分的复杂性，可能会影响 每个功能。提案研究的目的得到了研究培训，教学课程， 科学会议以及传播和未来增长的具体计划。最终，短期和长期 这项研究的目标是1）通过优化最生物活性人类的能力来改善婴儿健康 牛奶成分和2）确定人奶细胞保护婴儿并促进的机制 增长与发展。