Peptide-Polymer Engineering Dentin/Adhesive Interfacial Bond Integrity

肽-聚合物工程牙本质/粘合剂界面结合完整性

• 批准号: 10452710
• 负责人: Paulette Spencer
• 金额: $ 35.62万
• 依托单位: UNIVERSITY OF KANSAS LAWRENCE
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-08-12 至 2025-07-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10452710
• 关键词: Acids; Address; Adhesives; Adult; Amalgam; Anti-Bacterial Agents; Bacteria; Biodegradation; Characteristics; Child; Clinical; Composite Resins; Data; Dental Care; Dentin; Deterioration; Engineering; Environment; Enzymes; Failure; Feedback; Fracture; Hydrolysis; In Situ; Injury; Lead; Liquid substance; Longevity; Measurement; Mechanics; Mediating; Microbial Biofilms; Modeling; Oral; Oral cavity; Patients; Peptides; Performance; Plant Resins; Polymer Chemistry; Polymers; Process; Proliferating; Property; Psychological reinforcement; Recurrence; Resistance; Risk; Secondary to; Streptococcus mutans; Structure; System; Tissues; Tooth Loss; Tooth structure; Virulence; Viscosity; antimicrobial; base; cariogenic bacteria; chemical property; composite restoration; demineralization; design; high risk; improved; in vivo; interfacial; mechanical properties; meetings; monomer; novel; predictive modeling; remineralization; repaired; restoration; restorative material; seal

Project Summary The cycle of repeated composite-restoration replacements is a pernicious problem—each replacement risks pulpal injury, increased tooth weakness, and eventually, tooth loss. The problem is pervasive—nearly 70% of all composite restorations are replacements and the leading cause of failure is recurrent marginal decay. Unlike amalgam, composite lacks the inherent capability to seal discrepancies at the material/tooth interface. The adhesive that bonds the composite to the tooth is intended to seal the interface, but the adhesive seal to dentin is fragile—it is readily damaged by acids, enzymes, and other oral fluids. Bacteria and bacterial by-products infiltrate the resulting marginal gaps, demineralize and decompose the tooth, and further erode the adhesive, leading to wider and deeper gaps that create an ideal environment for bacteria to proliferate. Biodegradation by- products accumulate at the dentin/adhesive interface and increase the virulence of cariogenic bacteria, provoking a positive feedback loop that escalates the degradation. There is an urgent need to address this composite/tooth-interface vulnerability through multi-factorial approaches that: i) remineralize damaged dentin; ii) inhibit bacterial attack; and iii) provide durable polymers. Recent findings from our lab offer significant promise for meeting this need. First, we have synthesized novel self-strengthening polymers—the self-strengthening mechanism provides a persistent, intrinsic reinforcement of the polymer network in both neutral and acidic conditions. We have engineered antimicrobial and remineralizing peptides and tethered them to self- strengthening-adhesive monomers. Building on our progress, we propose to use both engineered peptides and antibacterial agents tethered to novel polymers to provide a “bio-hybrid” adhesive that will serve as a durable barrier to recurrent decay. Our threefold strategy exploits: (i) polymer-tethered engineered peptides and antibacterial agents to simultaneously provide antibacterial activity, delay biofilm formation, and remineralize dentin; (ii) self-strengthening polymers that resist hydrolysis-mediated degradation; and (iii) iterative feedback between synthesis, characterization, and modeling to forecast performance under relevant in vivo conditions.

项目摘要 重复组成替换的循环是一个有害问题 - 每个替代风险 牙髓损伤，牙齿无力增加和最终的牙齿丢失。 复合修复体是替代的，而失败的主要原因是反复的边缘衰变。 合并，复合材料缺乏在材料/牙齿界面上密封差异的固有能力 粘合到牙齿的粘合剂旨在密封界面，但是牙本质的粘合剂密封 是脆弱的 - 它很容易被酸，酶和其他口服流体损坏。 渗入牙齿的牙齿，进一步侵蚀粘合剂， 导致较宽，更深的缝隙，使细菌逐渐增殖。 产物积聚在牙本质/粘合剂界面上会增加致癌细菌的病毒性，即 激发一个积极的反馈循环，以升级降级。 复合/牙齿截距漏洞通过以下多因素方法：i）回忆dentin损坏的牙本质； ii）抑制细菌攻击 为了满足这种需求，我们已经合成了新颖的新型自我促进聚合物 机理提供了中性和酸性中聚合物网络的持续固有增强 条件。 加强粘合剂的单体。 抗菌剂束缚在新型聚合物上，以提供“生物杂交”粘合剂，可作为耐用 反复衰变的障碍。 抗菌剂同时提供抗菌作用，延迟生物膜形成并回忆起来 牙齿; 在相关的体内条件下的综合，表征和建模之间进行预测性能。