Electrokinetic lithography: in situ microengineering of anisotropic 3D collagen matrices

动电光刻：各向异性 3D 胶原基质的原位微工程

基本信息

批准号：
10452905
负责人：
Vinay V Abhyankar
金额：
$ 22.19万
依托单位：
ROCHESTER INSTITUTE OF TECHNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2022
资助国家：
美国
起止时间：
2022-06-01 至 2024-05-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10452905
关键词：
3-Dimensional Address Affect Architecture Biocompatible Materials Biomedical Research Biomimetics Biopolymers Cell Communication Cells Charge Collagen Collagen Fiber Collagen Type I Communication Cues Deposition Development Developmental Biology Disease Endothelial Cells Engineering Environment Epithelial Extracellular Matrix Fiber Future Gel Goals Heterogeneity Immune In Situ Laboratories Letters Malignant Neoplasms Microfluidics Mission Morphogenesis Motion National Institute of General Medical Sciences Outcome Population Predictive Value Process Program Development Property Proteins Reporting Research Research Support Resolution Shapes Speed Stimulus Surface System T-Lymphocyte Techniques Technology Testing Tissues Traction Work Writing base biofabrication bioprinting cancer cell cell motility design electric field fluid flow insight lithography magnetic field mechanical signal particle prototype real-time images response self assembly success technology development technology research and development tool tumor microenvironment

项目摘要

Summary In this project we will develop a technology called electrokinetic lithography (EKL) that will fill a technology void in the current state-of-the-art 3D aligned collagen fiber microengineering techniques. To mimic the structurally heterogenous environment found in the native extracellular matrix (ECM), we will sequentially combine extensional fluid flows with electric field driven bead motion (electrokinetics) to “write” cellular-scale discontinuities between domains of aligned collagen fibers within biomimetic 3D collagen gel. Technology development will be carried out with the followings aims: 1) Establish flow-based collagen fiber alignment and characterize electrokinetic transport parameters, and 2) Develop a microfluidic platform to engineer discontinuities within aligned 3D gel environments and validate cell motility responses. The success of this project will establish a transferrable lab prototype and support unprecedented studies that explore how cells respond to local disruptions in the aligned fibrous microarchitecture. Our technique will support new lines of exploration related to motility, sensing, and cell-cell communication within structurally heterogeneous environments, and address research questions cannot be currently answered with state-of- the-art collagen alignment techniques. This project directly aligns with the NIGMS mission of developing tools that enable potentially transformative biomedical research.

概括在这个项目中，我们将开发一项称为电动岩石摄影（EKL）的技术，该技术将填充一项技术在当前最新的3D排列胶原纤维微工程技术中的空隙。模仿在天然细胞外基质（ECM）中发现的结构异质环境，我们将顺序依次将伸展流体流与电场驱动珠运动（电动动力学）结合起来，以“写”蜂窝尺度仿生3D胶原蛋白凝胶中对齐胶原蛋白纤维的结构域之间的不连续性。技术以下目的将进行开发：1）建立基于流动的胶原蛋白纤维对齐和表征电子传输参数，2）开发一个微流体平台为工程师对齐的3D凝胶环境中的不连续性并验证细胞运动响应。该项目的成功将建立转移的实验室原型，并支持前所未有的研究探索细胞如何应对对齐的纤维微体系结构中的局部破坏。我们的技术会支持与运动性，传感和细胞电池通信有关的新的探索线异构环境和解决研究问题目前无法通过最新解决 ART胶原蛋白对准技术。该项目直接与开发工具的纽格姆斯任务保持一致这可以实现潜在的变革性生物医学研究。