Development of remotely actuated deformable membranes for in situ mechanical testing of soft tissue

开发用于软组织原位机械测试的远程致动变形膜

• 批准号: 10452283
• 负责人: Andres Arrieta Diaz
• 金额: $ 22.05万
• 依托单位: PURDUE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-23 至 2024-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10452283
• 关键词: 3-Dimensional; Acute; Algorithms; Animal Disease Models; Animal Model; Animals; Architecture; Basic Science; Big Data; Biomechanics; Biomedical Engineering; Biomedical Research; Chronic; Cicatrix; Complex; Computer software; Connective and Soft Tissue; Contracture; Data; Data Set; Dermal; Development; Devices; Diagnostic; Disease; Disease Progression; Elements; Environment; Exhibits; Frequencies; Future; Goals; Health; Human; Hypertrophic Cicatrix; In Situ; Investigation; Liver; Liver Fibrosis; Magnetic Resonance Imaging; Magnetism; Measurement; Measures; Mechanics; Membrane; Methods; Modeling; Monitor; Mus; Onset of illness; Organ; Output; Pathology; Pattern; Physiological; Process; Property; Reconstructive Surgical Procedures; Research; Residual state; Skin; Stress; Technology; Testing; Thinness; Tissue Expansion; Tissue Sample; Tissues; Validation; Vision; Work; base; biocompatible polymer; data-driven model; imaging modality; implantable device; improved; in vivo; kinematics; machine learning model; magnetic field; mechanical behavior; mechanical properties; new technology; non-invasive imaging; novel; particle; response; simulation; soft tissue; technology development; tissue phantom; tissue reconstruction; tissue stress; tool; ultrasound

Project Summary Soft connective tissues have remarkable mechanical functions, operating in the large deformation regime, showing highly nonlinear stress-strain response, and being physiologically under residual stress. Dysregulation of the tissue homeostatic state is associated with pathology, such as hypertrophic scar contracture and liver fibrosis. Progress in imaging modalities has opened a window into tissue kinematics in vivo in health and disease, for example high frequency ultrasound. Yet, measurement of mechanical properties in vivo remains out of reach. The state of the art in biomedical research remains ex vivo mechanical tests, which hinders progress in basic biomedical research towards understanding how tissues adapt mechanically in health and disease. Thus, for accurate measurement of the physiological mechanical environment of soft tissues, to better understand biomechanics of disease onset and progression, and eventually to improve diagnostics and treatment based on the evolving mechanics of soft tissue, new tools to measure mechanical properties in vivo are urgently needed. The objective of this proposal is to develop a novel remotely actuated deformable membrane to perform mechanical tests of soft tissue in vivo. We will develop a remotely actuated membrane capable of locally applying controlled stress fields to underlying tissue and measuring the ensuing deformation with high frequency ultrasound (Aim 1); develop a data-driven model of an active membrane adhered to a soft deformable substrate to enable parameter estimation from complex stress-strain data (Aim 2); and validate the technology on tissue phantoms, and murine skin and liver tissues ex vivo and in vivo (Aim 3). The work proposed here will result in a new technology to do in vivo mechanical tests of soft tissue, enabling progress of basic research in biomedical engineering. Development of this technology will open new possibilities to monitoring tissue mechanics in animal models of disease, expanding the current paradigm of kinematic tracking only. Our future work will continue in the direction of our long-term goal, towards development of implantable devices based on the same core technology proposed here.

项目摘要 软结缔组织具有显着的机械功能，在大变形方案中运行， 表现出高度非线性的应激应变反应，并在生理上显示在残留应力下。失调 组织稳态状态与病理学有关，例如肥厚疤痕和肝脏 纤维化。成像模式的进展为健康和疾病中的组织运动学开辟了一个窗口， 例如高频超声。然而，体内机械性能的测量仍然无法触及。 生物医学研究中的最新状态仍然是体内机械测试，这阻碍了基本的进展 生物医学研究，以了解组织如何机械适应健康和疾病。因此， 精确测量软组织的生理机械环境，以更好地了解 疾病发作和进展的生物力学，并最终基于 迫切需要在体内测量机械性能的新工具不断发展的力学。 该提案的目的是开发一种新型远程驱动的可变形膜以执行 体内软组织的机械测试。我们将开发一个能够在本地应用的远程驱动的膜 可控的应力场，以底层组织并以高频测量随后的变形 超声波（AIM 1）；开发一个粘附于软变形底物的活性膜的数据驱动模型 从复杂的应力 - 应变数据启用参数估计（AIM 2）；并验证组织上的技术 幻影，鼠皮肤和肝组织在体内和体内（AIM 3）。这里提出的工作将导致 新技术要在体内机械测试中的软组织，从而实现了生物医学基础研究的进展 工程。该技术的开发将为监测动物的组织力学开辟新的可能性 疾病模型，仅扩大运动学跟踪的当前范式。我们未来的工作将继续 我们长期目标的方向，基于相同核心开发可植入设备的方向 在这里提出的技术。