Protein-Glycan Interaction Resource at the National Center for Functional Glycomics (NCFG)

国家功能糖组学中心 (NCFG) 的蛋白质-聚糖相互作用资源

• 批准号: 10442455
• 负责人: RICHARD D CUMMINGS
• 金额: $ 96.25万
• 依托单位: BETH ISRAEL DEACONESS MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2020
• 资助国家: 美国
• 起止时间: 2020-07-01 至 2025-06-30
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10442455
• 关键词: Animals; Antibodies; Area; Autoimmunity; Bacteria; Binding; Binding Proteins; Biomedical Research; Cells; Collaborations; Communicable Diseases; Communities; Complex Mixtures; Coupling; DNA Library; Data; Databases; Development; Disease; Educational process of instructing; Ensure; Fluorescence; Funding; Generations; Glycobiology; Glycoconjugates; Glycolipids; Glycopeptides; Glycoproteins; Glycosaminoglycans; Health; High Pressure Liquid Chromatography; Human; Human Development; Immunology; Improve Access; Individual; Journals; Label; Laboratories; Lead; Lectin; Libraries; Malignant Neoplasms; Manuscripts; Microarray Analysis; Microbe; Modification; Molecular Conformation; Monitor; Monoclonal Antibody R24; National Institute of General Medical Sciences; Natural Resources; Oligosaccharides; Organism; Outcome; Peer Review; Peptide Library; Polysaccharides; Post-Translational Protein Processing; Printing; Production; Proteins; Publications; Publishing; RNA library; Recombinants; Research; Research Personnel; Resources; Services; Shotguns; Source; Sulfate; System; Technology; Time; Tissues; Virus; animal data; base; biological systems; cell type; density; glycosylation; gut microbiome; improved; innovation; inorganic phosphate; microbial; new technology; novel; outreach; pathogenic microbe; preservation; programs; success; tool; underserved area; web site

Abstract/Summary This R24 application for an NIGMS National and Regional Resource is to enable researchers in the under- served area of glycosciences and whose research needs glycoscience expertise, to have continued and improved access to state-of-the-art technologies to advance biomedical research and human health involving protein-glycan interactions and glycan recognition. Glycosylation is the most common and varied post- translational modification (PTM) in all living things, and each cell and organism generates unique PTMs and also glycolipids. Such resources proposed here are neither available to individual laboratories, nor are these specific technologies available commercially. With a base of ~7,000 users (83.6% new users) and hundreds of laboratories utilizing NCFG databases and resources, respectively, related to functional glycomics, our resource is obviously well used and represents a unique resource democratically accessible to all biomedical researchers. The increasing demand is evidenced by the nearly 10,000 individual, different glycan microarrays used at the NCFG resource center in just the last 4 years. Our emphasis on protein-glycan interactions is timely because research continues to uncover clues that these interactions are key to understanding the expression and functions of glycans in biological systems and their recognition by antibodies, glycan-binding proteins (GBPs) and lectins, in human and animal systems, and by microbial pathogens and gut microbiome. Our resource makes available an incredible variety of glycan microarray technologies, glycan libraries, and multiple databases not available elsewhere. The success of our resource has resulted in over a hundred publications by users in the past 5 years. Because of its success and rapidly increasing number of requests, and because we are the only resource of this kind available in the glycosciences, we propose three Specific Aims to continue making these invaluable resources accessible. Aim 1- We will continue to generate and improve defined glycan, glycopeptide, glycoprotein, and glycolipid microarrays, along with Luminex-based glycan-bead technologies, all unique to the resource. Aim 2- We will make available and further improve natural bifunctional fluorescent-tags (BFTs) originally pioneered by the NCFG, and will be utilized in glycan microarray and bead conjugations. Such novel BFTs, pioneered by the NCFG, give us advanced capabilities to label glycans in complex mixtures, purify them, and then quantify and quantitatively print microarrays at varying densities. Aim 3- We will continue to use BFT technology to produce and improve natural glycan microarrays from many sources of cells, tissues, and glycoconjugates, comprising a unique and valuable tool for the resource center. Thus, our Protein-Glycan Interaction Resource provides unique capabilities in glycan- binding expertise and technologies, teaching opportunities, and a wide range of services and outreach to an emerging field rapidly becoming recognized as a key factor in health and disease.

摘要/摘要 R24用于NIGMS国家和区域资源的申请是使研究人员能够 糖基镜的服务领域，其研究需要糖科学专业知识，以继续和 改善了获得最先进的技术，以推进生物医学研究和人类健康涉及 蛋白质 - 聚糖相互作用和聚糖识别。糖基化是最常见和最多样化的 所有生物中的翻译修饰（PTM），每个细胞和生物都会产生独特的PTM和 也是糖脂。此处提出的此类资源既不可用于个别实验室，也不可用 商业上可用的特定技术。基础约有7,000个用户（新用户83.6％）和数百个用户 利用NCFG数据库和资源的实验室，与功能性糖基质相关，我们 资源显然已被充分使用，代表了所有生物医学都可以访问的独特资源 研究人员。近10,000个不同的聚糖微阵列证明了需求不断增长的需求 在过去的四年中，在NCFG资源中心使用。我们对蛋白质 - 聚糖相互作用的强调是 及时，因为研究继续揭示了这些相互作用是理解这些相互作用的关键的线索 聚糖在生物系统中的表达和功能及其通过抗体识别，聚糖结合 蛋白质（Gbps）和凝集素，在人和动物系统中，通过微生物病原体和肠道微生物组。 我们的资源使各种各样的聚糖微阵列技术，聚糖图书馆和 多个数据库在其他地方没有可用。我们资源的成功导致了一百多个 在过去的5年中，用户的出版物。由于其成功并迅速增加了请求， 而且由于我们是糖基镜中唯一可用的资源，所以我们提出了三个特定的特定资源 旨在继续使这些宝贵的资源可以访问。目标1-我们将继续产生并 改善定义的聚糖，糖肽，糖蛋白和糖脂微阵列，以及基于Luminex的微阵列 Glycan珠技术，都是资源所独有的。目标2-我们将提供并进一步改善 最初由NCFG开创的天然双功能荧光标签（BFTS），并将用于聚糖 微阵列和珠子结合。由NCFG开创的这种新颖的BFT，为我们提供了高级功能 要在复杂的混合物中标记聚糖，请净化它们，然后在 密度不同。目标3-我们将继续使用BFT技术来生产和改善自然聚糖 来自许多细胞，组织和糖缀合物的微阵列，包括独特而有价值的工具 对于资源中心。因此，我们的蛋白质 - 聚糖相互作用资源为聚糖提供了独特的功能 具有约束力的专业知识和技术，教学机会以及广泛的服务，并向 新兴领域迅速被公认为是健康和疾病的关键因素。