600MHz NMR Spectrometer

600MHz核磁共振波谱仪

• 批准号: 10431284
• 负责人: Jason Jianxin Guo
• 金额: $ 156.61万
• 依托单位: NORTHEASTERN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-04-15 至 2024-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431284
• 关键词: Active Biological Transport; Aging; Automation; Bacteria; Bacterial DNA; Biological; Biological Products; Biology; Biomedical Research; Brain Diseases; Carbohydrates; Cell Extracts; Chemicals; Closure by clamp; DNA biosynthesis; Devices; Epithelial; Funding; Institutes; Kinetics; Lead; Mercury; NMR Spectroscopy; Natural Products; Oligonucleotides; Parasites; Proteins; United States National Institutes of Health; Universities; endogenous cannabinoid system; improved; innovation; instrument; medical schools; protein protein interaction; tool

Project Summary We propose to acquire a Bruker Avance Neo 2-channel 600 MHz NMR spectrometer with a BBFO CryoProbe and a SampleCase automation device, to be housed in the NMR Facility at the Barnett Institute of Northeastern University. This versatile configuration will transform the capabilities of the two aging and oversubscribed instruments in our NMR core, a 500 MHz Varian INOVA and a 400 MHz Varian Mercury Plus, to meet rapidly increasing demands for chemical and biological structural analysis. The cutting-edge CryoProbe and automation capabilities of the requested 600 MHz NMR will enable a range of innovative projects at the forefront of interdisciplinary biomedical research, leaving routine usage to the existing NMR instruments. The following NIH-funded projects of the major users of the instrument will benefit immediately from the new features of the instrument: Kinetic target-guided synthesis to modulate protein-protein interactions (R. Manetsch); Discovery of lead and tool compounds for probing and modulating protozoan parasite biology (M. Pollastri/L. Ferrins); de novo synthesis of biologically active natural products and carbohydrate motifs (G.A. O’Doherty); Targeting the endocannabinoid system in treating brain disorders (A. Makriyannis); Profiling secondary metabolites in cell extracts of uncultured bacteria (K. Lewis); Improving oligonucleotide biopharmaceuticals by steric protection (K. Zhang); Dynamics of processivity clamp proteins in bacterial DNA replication (P. Beuning); and Transport of biologically active molecules across tight epithelial barriers (W. Lencer, Harvard Medical School).

项目概要 我们建议购买一台 Bruker Avance Neo 2 通道 600 MHz NMR 波谱仪， BBFO CryoProbe 和 SampleCase 自动化设备，安装在 NMR 设施中 东北大学巴尼特研究所的这种多功能配置将改变 我们的 NMR 核心中两台老化且超额订购的仪器（一台 500 MHz）的功能 Varian INOVA 和 400 MHz Varian Mercury Plus，可满足快速增长的需求 化学和生物结构分析。 所要求的 600 MHz NMR 的尖端 CryoProbe 和自动化功能将 实现跨学科生物医学研究前沿的一系列创新项目， 保留现有 NMR 仪器的常规使用 以下 NIH 资助的项目。 该仪器的主要用户将立即受益于该仪器的新功能 仪器：动力学目标引导合成来调节蛋白质-蛋白质相互作用（R. 发现用于探测和调节原生动物的先导化合物和工具化合物 寄生虫生物学（M. Pollastri/L. Ferrins）；生物活性天然物质的从头合成 产品和碳水化合物基序（G.A. O’Doherty）；针对内源性大麻素系统 治疗脑部疾病（A. Makriyannis）；分析细胞提取物中的次生代谢物 未培养的细菌（K. Lewis）；通过空间改进寡核苷酸生物制药 细菌DNA复制中持续钳蛋白的保护（K.Zhang）； Beuning）；以及生物活性分子穿过紧密的上皮屏障的运输（W. 伦瑟，哈佛医学院）。