Two-photon laser-scanning microscope for interdisciplinary collaborations at Weill Cornell

威尔康奈尔大学用于跨学科合作的双光子激光扫描显微镜

• 批准号: 10431447
• 负责人: Conor M Liston
• 金额: $ 60万
• 依托单位: WEILL MEDICAL COLL OF CORNELL UNIV
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-07-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431447
• 关键词: Animals; Behavioral; Behavioral Assay; Calcium; Cells; Complex; Custom; Equipment; Grant; Image; Immunology; Lasers; Microbiology; Microscope; Nerve Degeneration; Neurons; Neurosciences; Physics; Research Personnel; Sapphire; Scanning; System; Time; design; developmental neurobiology; experimental study; fluorophore; in vivo imaging; innovation; instrument; instrumentation; interdisciplinary collaboration; multidisciplinary; neurodevelopment; neurophysiology; optical spectra; optogenetics; sensor; two-photon

Project Summary This is a proposal by investigators in neurophysiology, neurodevelopment, immunology, microbiology, and neurodegeneration for a Shared Instrumentation Grant to purchase an Olympus Fluoview Multiphoton Excitation Resonance Scanning (FVMPE-RS) Microscope equipped with two Spectra-Physics Ti:Sapphire Ultrafast Lasers. The Olympus FVMPE-RS is an innovative, state-of-the-art two-photon (2P) laser-scanning microscope that allows for simultaneous fluorophore excitation and imaging at two different wavelengths and is specially designed for in vivo imaging applications in neuroscience. By permitting the concurrent excitation of two genetically encoded calcium sensors with discernible emission spectra expressed independently in different neuronal subtypes, it is possible to assess the activation and interaction of different neuronal networks in behaving animals. The specially designed gantry system allows for 355 mm of clearance between the microscope objective and the baseplate, enabling users to install customized behavioral equipment for especially complex behavioral assays to be performed concurrently with in vivo imaging. The same equipment will also allow for simultaneous 2P imaging and 2P optogenetic stimulation, enabling the investigators not only to record from neuronal networks but also to manipulate their activity in real time, with single-cell precision. The requested instrument would enable Weill Cornell investigators to conduct multidisciplinary experiments spanning neurophysiology, developmental neurobiology, immunology, and microbiology.

项目概要 这是神经生理学、神经发育学、免疫学研究人员的提议， 微生物学和神经退行性疾病，以获得共享仪器补助金来购买 奥林巴斯 Fluoview 多光子激发共振扫描 (FVMPE-RS) 显微镜 配备两台 Spectra-Physics 钛宝石超快激光器。奥林巴斯 FVMPE-RS 是一款 创新、最先进的双光子 (2P) 激光扫描显微镜 在两种不同波长下同时进行荧光团激发和成像，特别是 专为神经科学中的体内成像应用而设计。通过允许并发 两个具有可辨别发射光谱的基因编码钙传感器的激发 在不同神经元亚型中独立表达，可以评估激活 以及行为动物中不同神经元网络的相互作用。特别设计的 龙门系统允许显微镜物镜和物镜之间有 355 毫米的间隙 底板，使用户能够安装定制的行为设备，以应对特别复杂的情况 行为测定与体内成像同时进行。相同的设备将 还允许同时进行 2P 成像和 2P 光遗传学刺激，从而使 研究人员不仅要记录神经元网络，还要操纵它们的活动 实时，具有单细胞精度。所请求的仪器将使威尔康奈尔大学能够 研究人员进行跨神经生理学、发育学等多学科实验 神经生物学、免疫学和微生物学。