Stratedigm S100EON high-parameter flow cytometer to support multi-institutional research programs

Stratigm S100EON 高参数流式细胞仪支持多机构研究项目

• 批准号: 10431674
• 负责人: Emanual M. Maverakis
• 金额: $ 59.98万
• 依托单位: UNIVERSITY OF CALIFORNIA AT DAVIS
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2024-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431674
• 关键词: Biological Assay; CLIA certified; California; Centrifugation; Cities; Clinic; Clinical Research; Comprehensive Cancer Center; Core Facility; Country; Diagnostic; Flow Cytometry; Freezing; Funding; Goals; Immunological Models; Immunology; Malignant Neoplasms; Memorial Sloan-Kettering Cancer Center; Preparation; Price; Process; Research; Research Personnel; Resource Sharing; Resources; Running; Sampling; San Francisco; Services; Specimen; Stains; System; Time; Travel; United States National Institutes of Health; Universities; Work; base; cost; instrument; prevent; programs

Title: Stratedigm S100EON high-parameter flow cytometer to support multi-institutional research programs Abstract: The Immune Modeling, Analysis, and Diagnostic Shared Resource (IMAD) is a CLIA- certified core facility that performs highly validated flow cytometry for various large multi- institutional NIH-funded research programs. It is the designated immunology core facility for the California Cancer Consortium, the UC Davis Alpha Clinic, and the UC Davis Comprehensive Cancer Center. The California Cancer Consortium is an NCI-funded consortium which includes investigators from the City of Hope, University of Southern California, University of California Davis, and Stanford. The UC Davis Alpha clinic runs local and multi-institutional CIRM and NIH-sponsored clinical studies. Its partners include the University of California San Diego, University of California San Francisco, and the Memorial Sloan Kettering Cancer Center. The goal of the current NIH S10 application is to support the purchase of the Stratedigm S100EON, which is a high-parameter flow cytometer. The extremely low operating cost of this instrument and its ability to fully automate the flow cytometry work flow (i.e. staining, centrifugation, incubation, loading, etc.) make this instrument especially suited for the IMAD and separate it from other the other flow cytometers currently available. These unique features will dramatically lower the per sample acquisition cost and increase the number of samples that can processed with IMAD's existing resources. Both lowering the per sample cost and increasing the throughput capacity are essential, if the IMAD is to meet their current demand, which is evident by their large backlog of frozen specimens. As it stands, the IMAD is possibly the only immunology core in the University of California system without a dedicated flow cytometer. Also, even if the IMAD investigators are willing to travel, there are no high-parameter flow analyzers available in Sacramento for them to use. The absence of a flow cytometer is the major bottleneck that prevents the IMAD from providing exceptional pricing and turnaround time for its users. The IMAD is focused on providing high throughput, highly validated flow-based assays to investigators across the country. Finally, IMAD highly validated sample preparation and acquisition services do not overlap with current UC Davis core facilities.

标题：S100EON高参数流式细胞仪，以支持多机构 研究计划 摘要：免疫建模，分析和诊断共享资源（IMAD）是一个Clia- 经过认证的核心设施，该设施执行高度验证的流式细胞仪，用于各种大型多数 机构NIH资助的研究计划。它是指定的免疫学核心设施 加利福尼亚癌症联盟，加州大学戴维斯加州大学阿尔法分校和加州大学戴维斯分校综合 癌症中心。加利福尼亚癌症联盟是一个由NCI资助的财团 加利福尼亚大学南加州大学希望之城的调查员 戴维斯和斯坦福。 UC Davis Alpha诊所运营本地和多机构的CIRM和 NIH赞助的临床研究。它的合作伙伴包括加州大学圣地亚哥分校 加利福尼亚大学旧金山大学和纪念斯隆·凯特林癌症中心。目标 当前的NIH S10申请是支持购买Stratedigm S100EON的购买 是高参数流式细胞仪。该工具的运营成本极低及其 能够完全自动化流式细胞仪工作流动（即染色，离心，孵育， 加载等）使该工具特别适合IMAD，并将其与其他工具分开 当前可用的其他流式细胞仪。这些独特的功能将大大降低Per 样本采集成本并增加可以与IMAD处理的样品数量 现有资源。同时降低样本成本和增加吞吐量的能力是 必不可少的，如果IMAD要满足他们当前的需求，这很明显 冷冻标本。就目前而言，IMAD可能是大学唯一的免疫学核心 没有专用流式细胞仪的加利福尼亚系统。另外，即使IMAD调查人员 愿意旅行，萨克拉曼多没有高参数流量分析仪 使用。缺乏流式细胞仪是阻止IMAD的主要瓶颈 为用户提供出色的定价和周转时间。 IMAD专注于 向研究人员提供高吞吐量，高度验证的基于流动的测定法 国家。最后，IMAD高度验证的样本准备和收购服务没有 与当前UC Davis核心设施重叠。