High-End Biological Atomic Force Microscope

高端生物原子力显微镜

• 批准号: 10431255
• 负责人: Tai-De Li
• 金额: $ 125.4万
• 依托单位: ADVANCED SCIENCE RESEARCH CENTER
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-16 至 2024-09-15
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10431255
• 关键词: Air; Arterial Fatty Streak; Biological; Biological Monitoring; Biological Phenomena; Biomedical Research; Biophysics; Biosensing Techniques; Core Facility; Diagnostic; Disease; Fluorescence; Funding; Future; Grant; Image; Immunology; Infrastructure; Institution; Manufacturer Name; Measurement; Measures; Mechanics; Microvascular Dysfunction; Minor; Molecular; Neurons; Optics; Positioning Attribute; Research; Research Personnel; Resolution; Safety; Sampling; Scanning Probe Microscopes; Science; Surface; System; Time; Tissues; Training; United States National Institutes of Health; Universities; biological systems; bone; experience; fluorescence microscope; instrument; mechanical properties; mechanotransduction; nanofluidic; novel strategies; operation; outreach; programs; tool

Project Summary This is a re-submission of the proposal “High-End Fluorescence Biological Atomic Force Microscope” of 2020 NIH S10 program (Application Number: 1 S10 OD030401-01) focusing on mechanobiological studies, a rapidly growing field of research on the influence of mechanics in biological systems. To study mechanobiology at the molecular, cellular, and tissue levels, researchers need a tool that can measure and control the mechanics of samples while monitoring biological activities within the samples. The instrument requested in this proposal is an integrated system that positions an atomic force microscope (AFM) on top of a fluorescence microscope, providing unique capabilities to precisely measure and apply forces and simultaneously visualize biological phenomena. The AFM platform offers force measurements, high-resolution mechanical property mapping, video- rate imaging, and a nano-fluidics integrated probe system. The fluorescence microscope includes a spinning disk confocal, total internal -reflection fluorescence (TIRF), and differential interference contrast (DIC). The PI and the respective manufacturers will integrate the two systems to set up a combined platform (Bio-AFM) that can perform advanced and simultaneous mechanical and optical measurements in real time, providing novel approaches for mechanobiological studies of a broad variety of target bio-systems. Access to the Bio-AFM will immediately advance the research of 16 users (10 major and 6 minor) from 5 universities and 11 departments, including 9 users funded by the NIH (> $12M in the past 5 years) and 6 users funded by NSF, Air Force, Navy, and Army research programs. Progress in those initial projects will advance research on neuron disorders (6 projects), the biophysics of atheroma and microvascular disease (3 projects), biomedical materials (3 projects), mechano-sensing and its application in immunology (2 projects), bone-related disorders (1 project), and bio-sensing for diagnostics (1 project). The Bio-AFM will be housed in a well-established core facility where it will be accessible to everyone that needs it. The Surface Science Core Facility currently serves more than 230 end users from more than 70 research groups. The existing infrastructure for instrument training, operation, and management is easily adaptable to accommodate the Bio-AFM. The PI is the Surface Science Core Facility’s founding Director (with more than 20 years of AFM research experience) and will be responsible for overseeing all Bio-AFM activities at the facility. The Bio-AFM, like all other instruments in the Surface Science Core Facility, will be fully supported by the institution with regard to finances, safety, outreach, IT, grant management, and administration. Given the expertise, infrastructure, and institutional supports, the requested Bio-AFM will not only benefit the initial 14 users, but also provide new opportunities to countless future users and new projects for biomedical research extending far beyond the end of the grant period.

项目摘要 这是提议“高端荧光生物原子力显微镜”提案的重新提出 NIH S10程序（申请号：1 S10 OD030401-01）专注于机械生物学研究，迅速 对机械系统在生物系统中的影响的研究领域。 分子，细胞和组织水平，研究人员需要工具可以测量和控制 在监测样品中的生物活动时，该提案中要求的仪器 将原子力显微镜（AFM）定位在荧光显微镜顶部的集成系统， 提供独特的独特功能来精确度量和应用福克斯，并同时可视化生物学 现象。 速率成像和纳米流体的探针系统。 磁盘共焦，总内部反射荧光（TIRF）和差异干扰对比度（DIC） 各个制造商将整合两个系统，以建立一个组合平台（Bio-AFM） 可以实时执行高级和同时的机械测量，提供新颖 多种目标生物系统的机械生物学研究方法。 访问Bio-Afm将立即将16位用户（10个专业和6个未成年人）的研究从5点提高 大学和11个部门，包括由NIH资助的9个用户（过去5年> 1200万美元）和6个用户 由NSF，空军，海军和陆军研究计划资助。 神经元疾病的研究（6个项目），动脉瘤和微血管疾病的生物物理学（3个项目） 生物医学材料（3个项目），机械感应，并应用于免疫学（2个项目），与骨有关 疾病（1个项目）和用于诊断的生物感应（1个项目）。 生物AFM将被安置在一个建立的核心设施中 IT。 小组。 可容纳生物AFM。 多年的AFM研究经验），并将负责监督该设施的所有生物AFM活动。 与表面科学核心设施中的所有其他乐器一样，生物AFM将得到您的全部支持 在财务，安全，外展，IT，赠款管理和行政管理方面，鉴于您的机构。 专业知识，基础架构和机构支持，所请求的生物AFM不仅将使最初的14个用户受益 但也为无数未来的用户和新项目提供新的机会，以扩展生物医学研究 远远超出了赠款期末。