Cytek Aurora Full Spectrum Flow Cytometer

Cytek Aurora 全谱流式细胞仪

• 批准号: 10429777
• 负责人: Yoav Altman
• 金额: $ 46.83万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-20 至 2024-06-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10429777
• 关键词: Accounting; Aging; Biological Process; Biomedical Research; California; Cells; Child; Childhood Medulloblastomas; Color; Coronavirus; Cytometry; Developmental Biology; Disease; Flow Cytometry; Fluorescence; Funding; Genomics; Gold; Gut Mucosa; Health; Healthcare; Heterogeneity; Immune; Immune Evasion; Immunooncology; Infection; Inflammation; Inflammatory; Institutes; Lasers; Malignant Neoplasms; Malignant neoplasm of pancreas; Measures; Medical; Molecular; Mucosal Immunity; Proteomics; Protocols documentation; Publications; Research Institute; Research Personnel; Running; Sampling; T-Lymphocyte; Technology; Time; Tissues; Tumor Immunity; United States National Institutes of Health; Universities; base; exhaustion; graft vs host disease; high standard; insight; malignant breast neoplasm; melanoma; single cell analysis; time use

PROJECT SUMMARY Sanford Burnham Prebys Medical Discovery Institute (SBP) has ~40 labs studying the molecular and cellular underpinnings of diverse biological processes including cancer; developmental biology; immuno-oncology; infection, inflammation; aging and related diseases. Many recent advances in health care depend upon new insights into tissue heterogeneity at the single cell level. Flow cytometry is the gold standard for high throughput single cell analysis, however conventional flow is limited by the number of analytes that can be run in unison. Spectral flow cytometers recently achieved the parameter depth required for deep immune profiling with publications showing 40+ color rivaling time-of-flight mass cytometry (CyTOF) parameter depth with ~50-fold higher throughput. SBP requests support for a Cytek Aurora Spectral Flow Cytometer with 5 lasers (UV/V/B/YG/R), 64 fluorescence and 3 scattering channels, and Automatic Micro-sampling Station (AMS) to enable high- parameter single-cell proteomic analysis for deep profiling of cell heterogeneity in tissues. We also request support for a BioBUBBLE Class I Negative Biocontainment Enclosure for Cytek Aurora to protect researchers running potentially infectious material. The Aurora more than doubles the number of fluorescent parameters that can be measured at SBP and leverages existing FACS protocols and expertise. It is a bridging technology between high-throughput low-plex conventional flow cytometers in SBP’s Core and high-plex low- throughput genomics-based single-cell Seq technologies. This application is supported by a coalition of 12 Major Users from SBP and nearby University of California San Diego (UCSD) and San Diego Biomedical Research Institute (SDBRI) demonstrating the broad impact for the region. NIH-funded researchers, together accounting for 75% of the Aurora’s available use time (AUT), need the Aurora to advance their understanding of T cell exhaustion, anti-tumor immunity, aging, multi-system inflammatory disease in children (MIS-C), gut mucosal immunity, melanoma, pancreatic cancer, breast cancer, pediatric medulloblastoma, graft versus host disease, and the immune evasion tactics of Coronavirus. The Aurora will provide unprecedented insight into tissue signatures that correlate with health and disease leading to new mechanistic inquiries and discoveries.

项目摘要 Sanford Burnham Prebys医学发现研究所（SBP）拥有约40个研究分子和细胞的实验室 包括癌症在内的潜水生物学过程的基础；发展生物学；免疫肿瘤学； 感染，感染；衰老和相关疾病。医疗保健的最新进展取决于新的 对单细胞水平的组织异质性的见解。流式细胞仪是高通量的金标准 单细胞分析，但是常规流程受到可以一致进行的分析数量的限制。 光谱流式细胞仪最近达到了深度免疫分析所需的参数深度 出版物显示40多个彩色色带飞行时间质量细胞术（Cytof）参数深度约50倍 更高的吞吐量。 SBP请求支持使用5个激光器（UV/V/B/YG/R）的Cytek Aurora光谱流式细胞仪，64 荧光和3个散射通道以及自动微采样站（AMS），以实现高 参数单细胞蛋白质组学分析，用于组织细胞异质性的深度分析。我们也要求 支持Cytek Aurora的生物泡I类负面生物内在围栏 研究人员运行潜在的传染性材料。 Aurora使荧光数量增加了一倍 可以在SBP上测量的参数并利用现有的FACS协议和专业知识。这是一个桥接 SBP核心中的高通量低音传统流式细胞仪与高音低 - 基于吞吐基因组学的单细胞SEQ技术。 该申请得到了来自SBP和附近加利福尼亚大学SAN的12位主要用户的联盟的支持 迭戈（UCSD）和圣地亚哥生物医学研究所（SDBRI）证明了对 地区。 NIH资助的研究人员共同占AURORA可用使用时间的75％（AUT），需要 Aurora提高他们对T细胞耗尽，抗肿瘤免疫学，衰老，多系统的理解 儿童炎症性疾病（MIS-C），肠粘膜免疫学，黑色素瘤，胰腺癌，乳腺癌， 小儿髓母细胞瘤，移植物与宿主疾病以及冠状病毒的免疫进化战术。这 Aurora将对与健康和疾病相关的组织特征提供前所未有的见解 新的机械查询和发现。