Bioluminescence and Fluorescence Optical Animal Imager

生物发光和荧光光学动物成像仪

• 批准号: 10419960
• 负责人: GUILLERMO MARQUES
• 金额: $ 44.73万
• 依托单位: UNIVERSITY OF MINNESOTA
• 依托单位国家: 美国
• 财政年份: 2022
• 资助国家: 美国
• 起止时间: 2022-09-01 至 2023-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10419960
• 关键词: 18 year old; 3-Dimensional; Affect; Animals; Autoimmunity; Bioluminescence; Cells; Detection; Drug Delivery Systems; Fluorescence; Funding; HIV; Image; Immune system; Immunotherapy; Ischemic Stroke; Laboratories; Mammary Neoplasms; Mus; Muscular Dystrophies; Optics; Physiological; Prostate Cancer therapy; Research; Research Personnel; Research Project Grants; Sampling; Systems Development; Time; United States National Institutes of Health; Vendor; antitumor drug; bioluminescence imaging; cancer therapy; flexibility; fluorescence imaging; imager; imaging capabilities; in vivo imaging system; instrument; multimodality; optical imager

Project Summary/Abstract The Perkin Elmer IVIS Spectrum requested is a whole animal optical imager capable of 2D and 3D fluorescence and bioluminescence imaging. The instrument provides high throughput (up to 10 mice at a time), high sensitivity (down to single cell detection), and a physiological platform that keeps the animals anesthetized and warm during imaging. Like other IVIS instruments, the IVIS Spectrum is calibrated in absolute radiance units, allowing comparison between samples imaged in different instruments. This instrument will replace an 18-year-old optical animal imager that is no longer supported by the vendor. More than 32 laboratories depend on the current instrument, and their research would be catastrophically affected if the instrument failed. Among those laboratories are the 10 NIH- funded investigators that are the users in this proposal. Being able to provide these investigators with the Perkin Elmer IVIS Spectrum animal imager would protect their current capabilities of bioluminescence and restore the fluorescence imaging capabilities available in the past. In addition the new instrument has better sensitivity and multimodal flexibility. The research projects thus enhanced will cover studies of immune system development and autoimmunity, surveillance of HIV CNS reservoirs, immune therapy of cancer, anti-tumor drug delivery in mammary and prostate cancer, and therapies for ischemic stroke and Duschenne’s muscular dystrophy.

项目摘要/摘要 所要求的Perkin Elmer IVIS频谱是能够2D的全动物光学成像器 和3D荧光和生物发光成像。该仪器提供高 吞吐量（一次最多10只小鼠），高灵敏度（直至单细胞检测）和A 在成像过程中使动物麻醉和温暖的生理平台。喜欢 其他IVIS仪器，IVIS频谱被以绝对的辐射单元进行校准，允许 在不同仪器中成像的样品之间的比较。该乐器将 替换不再由供应商支持的18岁的光学动物成像仪。 超过32个实验室取决于当前的工具，他们的研究将是 如果仪器失败，会对灾难性影响。在这些实验室中有10个NIH- 资助的调查员是本提案中的用户。能够提供这些 Perkin Elmer Ivis Spectrum动物成像师的研究人员将保护其 生物发光的当前功能并恢复荧光成像能力 过去可用。此外，新仪器具有更好的灵敏度和多模式 灵活性。因此，研究项目增强将涵盖免疫系统的研究 开发和自身免疫性，艾滋病毒中枢神经系统储藏的监视，免疫治疗 癌症，乳腺癌和前列腺癌中的抗肿瘤药物递送以及治疗 缺血性中风和杜森的肌肉营养不良。